Marine Sponge Endosymbionts: Structural and Functional Specificity of the Microbiome within
            <i>Euryspongia arenaria</i>
            Cells

Yang, Qi; Cahn, John W.; Piel, Jörn; Song, Yu; Zhang, Wei; Lin, Hou‐Wen

doi:10.1128/spectrum.02296-21

Cited by 8 publications

(7 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The predominant approach to study sponge holobiont was 16S. However, several studies featured shotgun metagenomic approaches, which shed light on sponge microbiome metabolic potential ( Table 1 , Yang et al, 2022 ; Moreno-Pino et al, 2020 , 2021 ). Sampling has been performed in all continents from the Southern Hemisphere, but mainly in Western Antarctic Peninsula ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Current evidence suggests that sponge microbiome is similar at the phylum level between species in the Southern and Northern Hemispheres ( Taylor et al, 2007 ). In the Southern Hemisphere, sponge microbiome is characterized by several core bacterial phyla, including Proteobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia, Acidobacteria, and Cyanobacteria ( Rodríguez-Marconi et al, 2015 ; Matcher et al, 2017 ; Cárdenas et al, 2018 , 2019 ; Savoca et al, 2019 ; Papale et al, 2020 ; Happel et al, 2022 ; Ruocco et al, 2021 ; Yang et al, 2022 ). Interestingly, Thaumarchaeota is the only archaea phylum associated with sponges, yet its association is consistent across several species ( Brochier-Armanet et al, 2008 ; Sacristán-Soriano, Pérez Criado & Avila, 2020 ; Moreno-Pino et al, 2020 ; Steinert et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Current knowledge of the Southern Hemisphere marine microbiome in eukaryotic hosts and the Strait of Magellan surface microbiome project

Ochoa-Sánchez,

Acuña Gomez,

Ramírez-Fenández

et al. 2023

PeerJ

View full text Add to dashboard Cite

Host-microbe interactions are ubiquitous and play important roles in host biology, ecology, and evolution. Yet, host-microbe research has focused on inland species, whereas marine hosts and their associated microbes remain largely unexplored, especially in developing countries in the Southern Hemisphere. Here, we review the current knowledge of marine host microbiomes in the Southern Hemisphere. Our results revealed important biases in marine host species sampling for studies conducted in the Southern Hemisphere, where sponges and marine mammals have received the greatest attention. Sponge-associated microbes vary greatly across geographic regions and species. Nevertheless, besides taxonomic heterogeneity, sponge microbiomes have functional consistency, whereas geography and aging are important drivers of marine mammal microbiomes. Seabird and macroalgal microbiomes in the Southern Hemisphere were also common. Most seabird microbiome has focused on feces, whereas macroalgal microbiome has focused on the epibiotic community. Important drivers of seabird fecal microbiome are aging, sex, and species-specific factors. In contrast, host-derived deterministic factors drive the macroalgal epibiotic microbiome, in a process known as “microbial gardening”. In turn, marine invertebrates (especially crustaceans) and fish microbiomes have received less attention in the Southern Hemisphere. In general, the predominant approach to study host marine microbiomes has been the sequencing of the 16S rRNA gene. Interestingly, there are some marine holobiont studies (i.e., studies that simultaneously analyze host (e.g., genomics, transcriptomics) and microbiome (e.g., 16S rRNA gene, metagenome) traits), but only in some marine invertebrates and macroalgae from Africa and Australia. Finally, we introduce an ongoing project on the surface microbiome of key species in the Strait of Magellan. This is an international project that will provide novel microbiome information of several species in the Strait of Magellan. In the short-term, the project will improve our knowledge about microbial diversity in the region, while long-term potential benefits include the use of these data to assess host-microbial responses to the Anthropocene derived climate change.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Current knowledge of the Southern Hemisphere marine microbiome in eukaryotic hosts and the Strait of Magellan surface microbiome project

Ochoa-Sánchez,

Acuña Gomez,

Ramírez-Fenández

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

“…The genus Burkholderia has demonstrated significant differences in CEs ( 50 ). Pseudomonas belongs to the lipase-producing bacteria and has been found only in the endosymbiotic flora, while Pseudomonas also shows a greater abundance inside the cell ( 51 ). Follow justly, Pseudomonas exhibited a significant difference in the number of CEs between LA and SA habitats.…”

Section: Discussionmentioning

confidence: 99%

Comparative Functional Genome Analysis Reveals the Habitat Adaptation and Biocontrol Characteristics of Plant Growth-Promoting Bacteria in NCBI Databases

Wang

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…While there are several studies investigating the functional gene repertoires of sponge holobionts (6)(7)(8)(9), there are relatively few studies that have interrogated the transcriptional profiles of these systems (10)(11)(12), and none focussing on bacterial symbionts at the genomeresolved level. A functional profile based on differential expression of genes of the HMA Xestospongia muta sponge holobiont was generated and showed that the associated bacteria had increased levels of transcripts associated with amino acid biosynthesis, while the host sponge transcribed genes for amino acid catabolism, suggesting a metabolic exchange between host and microbes (13).…”

Section: Introductionmentioning

confidence: 99%

Elevated Expression ofsrpRiPPs Across Bacterial Phyla in Marine Sponges

Waterworth

Rees

Clark

et al. 2023

Preprint

View full text Add to dashboard Cite

We investigated transcriptional activity, at a genome-resolved level, of bacterial communities in twoS. officinalisand twoI. felixsponges, both of which are considered high microbial abundance (HMA) sponges that harbor similar bacterial communities. Overlap of metatranscriptional data on genome-resolved metagenomic data showed that genome bins classified within the Chloroflexota and Poribacteria phyla were the most abundant and transcriptionally active. However, abundant bins in general were not the most transcriptionally active, instead less abundant bins of the same phyla were most active indicating that numerical dominance does not imply transcriptional dominance. We found that although some primary metabolic functions appeared upregulated, they were not obviously attributable to any particular bacterial species. However, assessment of transcription levels of biosynthetic gene clusters (BGCs) encoding secondary metabolites revealed a high transcription of ribosomally synthesized and post-translationally modified peptides (RiPPs) in genome bins across diverse bacterial phyla, most of which were likelysrpRiPPs encoding brominated azol(in)e-containing compounds. However, the ecological role of these compounds remains elusive.IMPORTANCEMarine sponges and bacteria have formed close associations over several million years with many bacteria performing specialized functions within their sponge host. Previous studies have either assessed the genomes of a given sponge or the expression profile for a sponge holobiont as a whole. Here, we present the first genome-resolved transcriptomic study which gives us a snapshot of the transcriptional activity of individual bacteria in the context of four different sponge holobionts. Using this approach we found that the numerically dominant bacteria were not the most transcriptionally active and that relatively high expression of a ubiquitous biosynthetic gene cluster was evident in many different bacterial phyla in all four sponge samples.

show abstract

Marine Sponge Endosymbionts: Structural and Functional Specificity of the Microbiome within Euryspongia arenaria Cells

Abstract: Sponges host abundant microbial symbionts that can produce an impressive number of novel bioactive metabolites. However, knowledge on intracellular (endosymbiotic) microbiota is scarce.

Cited by 8 publications

References 135 publications

Current knowledge of the Southern Hemisphere marine microbiome in eukaryotic hosts and the Strait of Magellan surface microbiome project

Current knowledge of the Southern Hemisphere marine microbiome in eukaryotic hosts and the Strait of Magellan surface microbiome project

Comparative Functional Genome Analysis Reveals the Habitat Adaptation and Biocontrol Characteristics of Plant Growth-Promoting Bacteria in NCBI Databases

Elevated Expression ofsrpRiPPs Across Bacterial Phyla in Marine Sponges

Contact Info

Product

Resources

About