In this paper, the mechanical properties and microstructures of AISI4140 low alloy steel under different tempering conditions are investigated. The samples are quenched, tempered to a martensite structure and loaded to fracture by means of Charpy machine according to standard test. Fractography analysis showed that the morphology fracture surface was changed by increasing tempering temperature. The variation of energy of Charpy impact fracture as a function of tempering temperature exhibits minimum values at 300 °C, which suggests the occurrence of temper embrittlement.