Marine polysaccharides: therapeutic efficacy and biomedical applications

Lee, Young‐Eun; Kim, Hyeongmin; Seo, Changwon; Park, Taejun; Lee, Kyung Bin; Yoo, Seung‐Yup; Hong, Seong‐Chul; Kim, Jeong Tae; Lee, Jaehwi

doi:10.1007/s12272-017-0958-2

Cited by 92 publications

(51 citation statements)

References 118 publications

(140 reference statements)

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“…Chitosan is a natural polymer derived from the alkaline N‐deacetylation of chitin, which is widely used in pharmaceutical and biomedical applications thanks to its useful characteristics of biodegradability, biocompatibility, low toxicity and moldability . Its molecular structure is very close to that of the glycosaminoglycans that compose the ECM, which renders it an ideal candidate as supportive material for the preparation of scaffolds intended for tissue engineering .…”

Section: Introductionmentioning

confidence: 99%

“…Chitosan is a natural polymer derived from the alkaline N-deacetylation of chitin, which is widely used in pharmaceutical and biomedical applications thanks to its useful characteristics of biodegradability, biocompatibility, low toxicity and moldability. [6][7][8] Its molecular structure is very close to that of the glycosaminoglycans that compose the ECM, which renders it an ideal candidate as supportive material for the preparation of scaffolds intended for tissue engineering. 9,10 In particular, the use of chitosan in oral and maxillofacial applications is particularly interesting because of its contribution to wound healing, 11,12 and its intrinsic antibacterial activity against some common oral pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Saccani

Parisi

Bergonzi

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Protein dynamics are fundamental for biological activity. Direct surface functionalization with these biomolecules often creates regions presenting non‐homogeneous and altered protein functionality. Aptamers have been shown to be an effective method to enhance the biocompatibility of biomaterials by acting as docking points for biomolecules capable of improving cell responses and the colonization/proliferation processes. Here mass spectrometry (MS) was successfully applied as an analytical approach to study the interactions between a fibronectin fragment (FN) and anti‐fibronectin aptamers in solution and on a chitosan film. Methods A 30 kDa N‐terminal fibronectin fragment, a ssDNA anti‐fibronectin 40 nucleotide long aptamer and chitosan 95/50 (degree of deacetylation 92.6–97.5%; molecular weight 80–220 kDa) were used. First, the dynamics of the FN in aqueous solution, in the presence or absence of anti‐FN‐specific DNA aptamers, were described. Thus, the same study was carried out on 2% (w/w) chitosan‐based films, functionalized with FN alone or through aptamers as selective spacers. Results Amide hydrogen/deuterium exchange mass spectrometry (HDX‐MS) analysis identified the SYRIGDTWSKKDNRGNL and YRVGDTYERPKDSMI, YVVGETWEKPYQGWMM and WERTYLGNAL fragments as presumably involved in the interaction of FN with aptamers. MS findings indicated the improved functionality of FN on chitosan when aptamers were used as selective spacer, and this may explain why cells preferentially attached to aptamer‐bound FN rather than on chitosan‐FN films. Conclusions Aptamers did not affect the amount of adsorbed FN, but influenced its conformation enhancing its biological activity toward adhesion and growth of osteoblasts. HDX‐MS data allowed the identification of the FN/aptamer interaction regions. Differences in FN flexibility on the chitosan film in the presence or absence of the aptamer were established providing insights at the molecular level to better understand the protein biological functionality on cell proliferation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Saccani

Parisi

Bergonzi

et al. 2019

Rapid Comm Mass Spectrometry

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“…Recent in vivo and in vitro studies indicated that fucoidan is a potent anti-tumor agent in cancer cells (e.g., colon, liver, bladder and breast cancer) [29][30][31][32][33][34][35]. However, no study has reported the relationship of ULs and fucoidan.…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Chen

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effect of fucoidan on the growth of ULs activated by transforming growth factor beta (TGFβ). Methods: We used ELT-3 (Eker rat leiomyoma tumor-derived cells) and HUtSMC (human uterine smooth muscle cells) as in vitro models. Cell viability was determined by the MTT assay. Cell colony formation was stained using crystal violet. The side population, cell cycle and apoptosis were analyzed using flow cytometry. Protein expression was assayed by western blot analysis. We also conducted in vivo experiments to confirm the inhibitory effects of fucoidan in nude mouse xenograft models. Tumor tissues were assayed by immunohistochemistry analysis. Results: In our study, fucoidan caused a 50% growth inhibition using a dose of 0.5 mg/ml and decreased the stem cell activity after 48 h. In addition, fucoidan induced sub-G1 cell cycle arrest and apoptosis. Fucoidan down-regulated fibronectin, vimentin, α-SMA and the COL1A1 protein levels in TGFβ3-induced ELT-3 cells. In the cellular mechanism, fucoidan abrogated TGFβ3-induced levels of p-Smad2 and p-ERK1/2, as well as β-catenin translocation into the nucleus. Furthermore, fucoidan suppressed xenograft tumor growth in vivo. Conclusion: Fucoidan displays anti-proliferation and anti-fibrotic effects and exerts protective effects against ULs development.

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“…Saccharides have long been used in the biomedical field, including drug delivery, gene delivery, cancer therapy and so on. Important properties of saccharides as nanomaterials include biodegradability, biocompatibility, nontoxicity and bioactivity (Lee et al, ; Manivasagan & Oh, ). A large variety of saccharides have been investigated and applied in clinical trials, such as fucoidan and chitosan.…”

Section: Organic Polymersmentioning

confidence: 99%

Detoxification and functionalization of gold nanorods with organic polymers and their applications in cancer photothermal therapy

Jin

Zhang

Yang

et al. 2019

Microscopy Res & Technique

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Gold nanorods (AuNRs) have attracted widespread attention in the last few decades due to their potential applications in cancer photothermal therapy (CPTT). However, the utilization of AuNRs is restricted because of their high toxicity for cells and tissues. Numerous methods have been developed to solve this problem, among which the most convenient one is to coat AuNRs with biocompatible materials or replace their original toxic surfactant such as the hexadecyl‐trimethyl ammonium bromide (CTAB). Organic polymers (OPs) are intensively researched due to their low toxicity and easy access to modification with functional groups (e.g., targeting molecules), pharmaceuticals (e.g., chemo drugs) and imaging agents (e.g., radioactive elements). Herein, this mini review summarized recent progress in detoxification and functionalization of AuNRs exploiting OPs coatings and their applications in CPTT. To begin with, we introduced current major approaches used to modify AuNRs with typical OPs. Then, applications of OPs coated AuNRs in CPTT were discussed. At the end of the review, we proposed a brief outlook for the future development of modification and functionalization of AuNRs with organic materials.

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Marine polysaccharides: therapeutic efficacy and biomedical applications

Cited by 92 publications

References 118 publications

Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Detoxification and functionalization of gold nanorods with organic polymers and their applications in cancer photothermal therapy

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