ABSTRACT. Six charophyte, 13 mollusc, four ostracod and nine ®sh otolith taxa are taxonomically described, and one fruit, one seed and two foraminiferal taxa are brie¯y noted from the Lower Cyrena Beds and the Lower Coloured Molasse of the Sindelsdorf section near Penzberg (approximately 50 km south of Munich). Our palaeoecological and lithological data from the Lower Cyrena Beds suggest a delta plain with lagoons, estuaries, slowly¯owing rivers, lakes and swamps. Faunal and¯oral elements of the Lower Coloured Molasse indicate lacustrine environments. The gastropod Tympanotonos and the tropical to subtropical ®sh fauna (Eleotridae, Ambassidae and Cyprinodontidae) suggest a warm, at least subtropical climate. Furthermore, Tympanotonos suggests comparisons with Recent molluscan faunas of the mangrove swamps of the West African coast, and thus hints at mangrove vegetation bordering the coasts of the Upper Bavarian Molasse Sea. A biostratigraphical classi®cation for the Oligocene Molasse deposits of the Penzberg Syncline is established for the ®rst time based on otoliths and charophytes. The Lower Cyrena Beds are attributed to the newly de®ned otolith zone OT-O1/2 and probably correspond to the oldest part of the Chara microcera Zone. The lowermost part of the Lower Coloured Molasse can be correlated both with otolith zone OT-O2 and the Chara microcera Zone. The Sindelsdorf section lies within the Rupelian±Chattian transition zone and thus the chronostratigraphic age is approximately 29±28 Ma.KEY WORDS: continental Oligocene, South German Molasse Basin, charophytes, molluscs, ostracods, ®sh otoliths.C O N T I N E N T A L deposits of Oligocene age in the South German Molasse Basin are herein considered on a broad palaeontological basis for the ®rst time. The section studied, situated in the Penzberg Syncline (Text-®gs 1±2), is 170 m thick and can be correlated with the Lower Cyrena Beds (Lower Brackish-Water Molasse) and the lowermost part of the Lower Coloured Molasse (Lower Freshwater Molasse) (Text-®g. 3). Charophytes, molluscs, ostracods and ®sh otoliths are taxonomically described, and fruits, seeds and foraminifers are brie¯y noted. Our palaeoecological analysis suggests varying environments in a tropical to subtropical climate. Biostratigraphical analysis based on the charophyte¯ora and ®sh otoliths suggests a latest Rupelian and/or earliest Chattian age for the succession.Previous studies of charophytes and ®sh otoliths from the lower Chattian of the Marienstein and Murnau synclines in the Southern German Molasse Basin include those published in Reichenbacher and Schwarz (1997), Uhlig et al. (2000) and Reichenbacher and Uhlig (2002). The Oligocene molluscs from this region have been studied by Zo Èbelein ( , 1955( ), Ho Èlzl (1957 and Barthelt (1989), and the ostracods by Witt (1967) and Mu Èller (1985). However, none of these publications dealt with the Lower Cyrena Beds in the Penzberg Syncline. Barthelt (1989) considered the depositional environment represented by the Lower Brackish-Water Molasse. She reco...