Marine Fungi Associated with Antarctic Macroalgae

Ogaki, Mayara Baptistucci; Paula, Maria T. de; Ruas, Daniele; Pellizzari, Franciane; García-Laviña, César X.; Rosa, Luiz Henrique

doi:10.1007/978-3-030-02786-5_11

Cited by 16 publications

(15 citation statements)

References 44 publications

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“…Several of the basidiomycete OTUs clustered with reference sequences of Cryptococcus and Cystofilobasidium that have previously been found associated to various marine invertebrates, algae and seaweeds (Fig. 5) [107][108][109][110]. In addition, within Chytridiomycota there were several OTUs that clustered with Rhizophydium sp., which comprise parasites with broad host ranges known to infect both macroalgae and protists [111,112].…”

Section: Novel Diversity and Putative Parasitesmentioning

confidence: 68%

Elucidating the diversity of microeukaryotes and epi-endophytes in the brown algal holobiome

Bjorbækmo

Brodie

Logares

et al. 2021

Preprint

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Background: Brown algae (Phaeophyceae) are essential species in coastal ecosystems where they form kelp forests and seaweed beds that support a wide diversity of marine life. Host-associated microbial communities are an integral part of phaeophyte biology. The bacterial microbial partners of phaeophytes have received far more attention than microbial eukaryotes. The pre-requisite to understand the ecology of phaeophytes and their host-associated microbes is to know their diversity, distribution and community dynamics. To our knowledge, this is the first study to investigate phaeophyte-associated eukaryotes (the eukaryome) using broadly targeting pan-eukaryotic primers and high throughput sequencing (HTS). Using this approach, we aimed to unveil the eukaryome of seven large common phaeophyte species. We also aimed to assess whether these macroalgae harbour novel eukaryotic diversity and to ascribe putative functional roles to the host-associated eukaryome, based on taxonomic affiliation and phylogenetic placement. Results: Our sequence dataset was dominated by phaeophyte reads, from the host species and potential symbionts. We also detected a broad taxonomic diversity of eukaryotes in the phaeophyte holobiomes, with OTUs taxonomically assigned to ten of the eukaryotic major Kingdoms or supergroups. A total of 265 microeukaryotic and epi-endophytic operational taxonomic units (OTUs) were defined, using 97% similarity cut off during clustering, and were dominated by OTUs assigned to Stramenopiles, Alveolata and Fungi. C Almost one third of the OTUs we detected have not been found in previous molecular environmental surveys, and represented potential novel eukaryotic diversity. This potential novel diversity was particularly diverse in phylogenetic groups comprising heterotrophic and parasitic organisms, such as labyrinthulids and Oomycetes, Cercozoa, and Amoebozoa. Conclusions: Our findings provide important baseline data for future studies of seaweed-associated microorganisms, and demonstrate that microeukaryotes and epi-endophytic eukaryotes should be considered as an integral part of phaeophyte holobionts. The potential novel eukaryotic diversity we found and the fact that the vast majority of macroalgae in marine habitats remain unexplored, demonstrates that brown algae and other seaweeds are potentially rich sources for a large and hidden diversity of novel microeukaryotes and epi-endophytes.

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Section: Novel Diversity and Putative Parasitesmentioning

confidence: 68%

Elucidating the diversity of microeukaryotes and epi-endophytes in the brown algal holobiome

Bjorbækmo

Brodie

Logares

et al. 2021

Preprint

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“…Macroalgae are a highly represented group in the marine environment, mainly in coastal ecosystems, and are considered the second largest source of marine fungi, after corals, in this type of environment [1]. The nutrient cycling promoted by macroalgae is crucial for the stability of coastal ecosystems [2].…”

Section: Introductionmentioning

confidence: 99%

“…[8] which detected 64 fungal taxa in the green alga Flabellia petiolata on the Italian coast. The fungal community in macroalgae can be influenced by biotic and abiotic factors [9], such as the production of antifungal compounds by the host [2], salinity, water levels and air exposure [10, 11].…”

Section: Introductionmentioning

confidence: 99%

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Diversity of fungi associated with macroalgae from an estuarine environment and description of Cladosporium rubrum sp. nov. and Hypoxylon aveirense sp. nov.

Vicente

Gonçalves

Brandão

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Fungal communities associated with macroalgae remain largely unexplored. To characterize algicolous fungal communities using culture dependent methods, macroalgae were collected from different sampling sites in the Ria de Aveiro estuary, Portugal. From a collection of 486 isolates that were obtained, 213 representative isolates were selected through microsatellite-primed PCR (MSP-PCR) fingerprinting analysis. The collection yielded 33 different genera, which were identified using the ITS region of the rDNA. The results revealed that the most abundant taxa in all collections were Acremonium-like species: Alternaria, Cladosporium, Leptobacillium and Penicillium. The fungal community composition varied with macroalgae species. Through multilocus phylogenetic analyses based on ITS, tub2, tef1-α and actA sequences, in addition to detailed morphological data, we propose Cladosporium rubrum sp. nov. (type strain=CMG 28=MUM 19.39) and Hypoxylon aveirense sp. nov. (type strain=CMG 29=MUM 19.40) as novel species.

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“…Cold-adapted fungi from the genus Pseudogymnoascus are prevalent in most of the Antarctic environments (Ogaki et al, 2019; Rosa et al, 2019). To date, the species P. appendiculatus , P. destructans , P. pannorum , P. verrucosus , and P. vinaceous have been isolated from different Antarctic environments (Loque et al, 2010; Gomes et al, 2018; Alves et al, 2019; Kochkina et al, 2019; Rosa et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin

et al. 2019

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Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin.

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Marine Fungi Associated with Antarctic Macroalgae

Cited by 16 publications

References 44 publications

Elucidating the diversity of microeukaryotes and epi-endophytes in the brown algal holobiome

Elucidating the diversity of microeukaryotes and epi-endophytes in the brown algal holobiome

Diversity of fungi associated with macroalgae from an estuarine environment and description of Cladosporium rubrum sp. nov. and Hypoxylon aveirense sp. nov.

Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin

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