Marine-derived collagen biomaterials from echinoderm connective tissues

Ferrario, C.; Leggio, Livio; Leone, Roberta; Benedetto, Cristiano Di; Guidetti, Luca; Coccè, Valentina; Ascagni, Miriam; Bonasoro, F.; Porta, Caterina A. M. La; Carnevali, M. Daniela Candia; Sugni, Michela

doi:10.1016/j.marenvres.2016.03.007

Cited by 53 publications

(76 citation statements)

References 54 publications

(21 reference statements)

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“…() (65.7 ± 0.5 nm), but they are higher and less variable than those reported by Ferrario et al. () (62.7 ± 2.8 nm for sea urchin, 63 ± 4.7 nm for starfish and 66 ± 1.6 nm for sea cucumber) and Ribeiro et al. () (59.2 ± 6.2 nm for sea urchin).…”

Section: Discussionmentioning

confidence: 56%

Body wall structure in the starfish Asterias rubens

et al. 2017

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The body wall of starfish is composed of magnesium calcite ossicles connected by collagenous tissue and muscles and it exhibits remarkable variability in stiffness, which is attributed to the mechanical mutability of the collagenous component. Using the common European starfish Asterias rubens as an experimental animal, here we have employed a variety of techniques to gain new insights into the structure of the starfish body wall. The structure and organisation of muscular and collagenous components of the body wall were analysed using trichrome staining. The muscle system comprises interossicular muscles as well as muscle strands that connect ossicles with the circular muscle layer of the coelomic lining. The collagenous tissue surrounding the ossicle network contains collagen fibres that form loop‐shaped straps that wrap around calcite struts near to the surface of ossicles. The 3D architecture of the calcareous endoskeleton was visualised for the first time using X‐ray microtomography, revealing the shapes and interactions of different ossicle types. Furthermore, analysis of the anatomical organisation of the ossicles indicates how changes in body shape may be achieved by local contraction/relaxation of interossicular muscles. Scanning synchrotron small‐angle X‐ray diffraction (SAXD) scans of the starfish aboral body wall and ambulacrum were used to study the collagenous tissue component at the fibrillar level. Collagen fibrils in aboral body wall were found to exhibit variable degrees of alignment, with high levels of alignment probably corresponding to regions where collagenous tissue is under tension. Collagen fibrils in the ambulacrum had a uniformly low degree of orientation, attributed to macrocrimp of the fibrils and the presence of slanted as well as horizontal fibrils connecting antimeric ambulacral ossicles. Body wall collagen fibril D‐period lengths were similar to previously reported mammalian D‐periods, but were significantly different between the aboral and ambulacral samples. The overlap/D‐period length ratio within fibrils was higher than reported for mammalian tissues. Collectively, the data reported here provide new insights into the anatomy of the body wall in A. rubens and a foundation for further studies investigating the structural basis of the mechanical properties of echinoderm body wall tissue composites.

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Section: Discussionmentioning

confidence: 56%

Body wall structure in the starfish Asterias rubens

et al. 2017

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“…After 3-4 days in the disaggregating β-mercapto-ethanol solution, the collagen fibers obtained are then removed by a filtration step and dialyzed against a 0.5 M EDTA-Na solution. The same protocol is used to extract collagen fibers from starfish aboral arm walls, but the samples are processed with an additional step in 1 mM citric acid between decellularizing and disaggregating solutions to remove the calcium carbonate ossicles present in the fresh tissue [75].…”

Section: Extraction Of Marine Collagenmentioning

confidence: 99%

“…They are well-known for their unique connective tissues, named mutable collagenous tissues (MCT), recently proposed as possible inspiration for "smart dynamic biomaterials" for tissue engineering and regenerative medicine applications [74,123]. It can be used to develop collagen barrier-membranes for guided tissue regeneration (GTR) [75].…”

Section: Echinodermsmentioning

confidence: 99%

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

et al. 2020

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Due to its unique properties, collagen is used in the growing fields of pharmaceutical and biomedical devices, as well as in the fields of nutraceuticals, cosmeceuticals, food and beverages. Collagen also represents a valid resource for bioplastics and biomaterials, to be used in the emerging health sectors. Recently, marine organisms have been considered as promising sources of collagen, because they do not harbor transmissible disease. In particular, fish biomass as well as by-catch organisms, such as undersized fish, jellyfish, sharks, starfish, and sponges, possess a very high collagen content. The use of discarded and underused biomass could contribute to the development of a sustainable process for collagen extraction, with a significantly reduced environmental impact. This addresses the European zero-waste strategy, which supports all three generally accepted goals of sustainability: sustainable economic well-being, environmental protection, and social well-being. A zero-waste strategy would use far fewer new raw materials and send no waste materials to landfills. In this review, we present an overview of the studies carried out on collagen obtained from by-catch organisms and fish wastes. Additionally, we discuss novel technologies based on thermoplastic processes that could be applied, likewise, as marine collagen treatment.2 of 23 protective colloid functions and film-forming capacity. Also, collagen is a good surface-active agent, with its ability to penetrate lipid-free interfaces [2].Collagen can be utilized in a variety of applications because of its biocompatibility and excellent degradability [3,4]. Furthermore, it is known that collagen is a molecule with weak immunogenicity, which decreases the possibilities of rejection when it is ingested or injected into a different body. Although this molecule has already low antigenicity, this property can be enhanced by modifying it to suppress any immune response [5,6]. Additionally, collagen peptides and gelatin (denatured collagen) have been widely utilized in different fields such as food, medicine, cosmetics, leather and film industries, diagnostic imaging, and therapeutic delivery [7].For many years, most available collagen was extracted from discards from the bovine and porcine processing industries, but during the last few decades the use of collagen from these sources has been limited. The use of porcine and bovine-derived products is occasionally prevented by dietary regimes, due to specific needs or personal choices. It is forbidden by religious constraints, to Muslims, Hindus and Jews who make up 38.4% of the global population [8]. Moreover, the use of bovine-derived products became a concern for a wider section of the population during the bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE) and foot-and-mouth disease (FMD) crises that occurred over the last few decades in all areas of the world, mostly in the United Kingdom and Asia. Bovine-derived products might be a vehicle of transmission of these diseases ...

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“…The properties of echinoderm MCT, although remarkably utilitarian, are not found in any other phylum (Wilkie 1984). The mechanical properties of MCT have been considered for their direct application to connective tissue improvement in humans and the development of novel biomaterials (Wilkie 2005;Barbaglio et al 2012Barbaglio et al , 2013Ferrario et al 2017;Goh & Holmes 2017).…”

Section: Collagenous Tissuementioning

confidence: 99%

<strong>Ophiuroid locomotion from fundamental structures to integrated systems</strong>

Clark

2019

Zoosymposia

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The phylum Echinodermata includes an estimated 7,000 extant and 13,000 extinct species. Each living class represents a separate body plan with a unique mode of movement and locomotion. Brittle stars (Class Ophiuroidea) utilize complex arm musculoskeletal physiology for rapid locomotion, in contrast to the other echinoderm classes, which are typically slow moving. Ophiuroid locomotion and its origins are poorly understood. This paper is a review of the current state of ophiuroid research, with a focus on topics relevant to ophiuroid movement and locomotion, including anatomy, physiology, functional morphology, disparity, ecology, and evolutionary history.

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Marine-derived collagen biomaterials from echinoderm connective tissues

Cited by 53 publications

References 54 publications

Body wall structure in the starfish Asterias rubens

Body wall structure in the starfish Asterias rubens

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

<strong>Ophiuroid locomotion from fundamental structures to integrated systems</strong>

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