Marine Collagen Peptides from the Skin of Nile Tilapia (Oreochromis niloticus): Characterization and Wound Healing Evaluation

Abstract

Burns can cause tremendous economic problems associated with irreparable harm to patients and their families. To characterize marine collagen peptides (MCPs) from the skin of Nile tilapia (Oreochromis niloticus), molecular weight distribution and amino acid composition of MCPs were determined, and Fourier transform infrared spectroscopy (FTIR) was used to analyze the chemical structure. Meanwhile, to evaluate the wound healing activity, in vitro and in vivo experiments were carried out. The results showed that MCPs prepared from the skin of Nile tilapia by composite enzymatic hydrolysis were composed of polypeptides with different molecular weights and the contents of polypeptides with molecular weights of less than 5 kDa accounted for 99.14%. From the amino acid composition, the majority of residues, accounting for over 58% of the total residues in MCPs, were hydrophilic. FTIR indicated that the main molecular conformations inside MCPs were random coil. In vitro scratch assay showed that there were significant effects on the scratch closure by the treatment of MCPs with the concentration of 50.0 μg/mL. In the experiments of deep partial-thickness scald wound in rabbits, MCPs could enhance the process of wound healing. Therefore, MCPs from the skin of Nile tilapia (O. niloticus) have promising applications in wound care.

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“…glycyrrhizic acid and glabridin (Yip, Poh, & Chia, 2016). In an earlier study, tilapia skin collagen peptides expressed no obvious effect on the scratch closure at 50 µg/mL concentration after 6 h, whereas, significant wound closure was noticed after 12 h, 18 and 24 h treatment in HaCaT cells Hu et al (2017).…”

“…In vitro scratch assay was performed to determine the wound healing based on the method of Hu et al (2017) with slight modification. For the assay, 3T3-L1 cells were were grown in DMEM supplemented with 10% FBS for 24 h. The cells were seeded into 24-well tissue culture plate at a density of 0.05 x 10 6 and grown for 24 h until cell growth reached 70-80% confluence as a monolayer.…”

“…Zhang et al (2011) investigated the effects of skin gelatin obtained from the chum salmon (Oncorhynchus keta) on defective wound repair in the skin of diabetic rats for 14 days by scratch assay and reported that wound closure occurred within 14 days along with reduction in inflammatory response. Hu, Yang, Zhou, Li and Hong (2017) have also performed scratch assay to examine wound healing effect of collagen peptides derived from tilapia fish skin (Oreochromis niloticus) and observed that HaCaT cells migration caused wound closure within 24 h.…”

Wound dressings: Current advances and future directions

“…(e1) Microscopical image of coagulation necrosis of skin layers, and (e2) the focal necrosis, adapted with permission from ref. . [Color figure can be viewed at wileyonlinelibrary.com]…”

“…The result confirmed that the tilapia‐collagen nanofibers could be used as an efficient and innovative wound dressings, which are cost‐efficient, and can eliminate from the transmission of disease originated from animals in clinical application. Hu et al . fabricated marine collagen peptides (MCPs) by the extraction of collagen from the Nile tilapia.…”