Marine Algae Incorporated Polylactide Acid Patch: Novel Candidate for Targeting Osteosarcoma Cells without Impairing the Osteoblastic Proliferation

Abstract

Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. In recent years, incorporation of marine algae in synthetic polymers has been considered as an alternative method for preparation grafts/scaffolds since they represent abundant and cheap source of potential biopolymers. Current work aims to propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) to polylactide (PLA) as an alternative to the porcine-derived membranes. SVP-PLA composite patches were produced by using a modified solvent casting method. Following detailed material characterization to assess the cytocompatibility, human osteoblasts (HOBs) and osteosarcoma cells (SaOS-2) were seeded on neat PLA and SVP-PLA patches. MTT and BrdU assays indicated a greater cytocompatibility and higher proliferation for HOBs cultured on SVP-PLA composite than for those cultured on neat PLA. SaOS-2 cells cultured on SVP-PLA exhibited a significant decrease in cell proliferation. The composite patch described herein exhibits an antiproliferative effect against SaOS-2 cells without impairing HOBs’ adhesion and proliferation.

Antibacterial properties of marine algae incorporated polylactide acid membranes as an alternative to clinically applied different collagen membranes

“…All marine algae -PLA composite membranes experimented herein showed suitable cytocompatibility as BrdU and MTT assays revealed. The results were equal to those of clinically applied RCMs, which confirms the results of the previous studies of our research group [9,10]. In general, it is known that various types of marine algae are cytocompatible polymers that can be used for biomaterial development.…”

“…In this regard, marine algae are of great interest as a source of natural biopolymers due to their abundance and inexpensive extraction [8]. In the past years, a wide spectrum of bioactivities such as antitumoral, antiviral, antioxidant and cell proliferative effects have been observed which makes them ideal biomaterials for medical applications [9,10]. After being processed into powders they can be blended with polyesters to enhance their biomechanical properties and thereby increase their structural integrity which enables medical applications like membranes for periodontal and implantology surgery [11].…”

Alginate/pectin dressing with niosomal mangosteen extract for enhanced wound healing: evaluating skin irritation by structure-activity relationship

“…For the high-dose patches ( Figure 10 E), fibroblast development was inhibited. Additionally, the cells remained spherical and lacked filopodia, indicating the patches' non-cytocompatibility [ 89 ]. This was due to ME's toxicity at higher concentrations.…”