Marine Actinobacteria: New Horizons in Bioremediation

Rathore, Dalip Singh; Singh, Satya Prakash

doi:10.1007/978-981-15-4439-2_20

Cited by 11 publications

(14 citation statements)

References 124 publications

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“…Brachybacterium species have previously been reported in association with sponges ( Kiran et al, 2014 ; Liu et al, 2019b ), including deep-sea sponges from the Antarctic ( Xin et al, 2011 ). While marine Actinobacteria have been reported to be capable of remediation of environmental pollutants including petroleum hydrocarbons and plastics ( Rathore et al, 2021 ); members of the genus Brachybacterium have also been shown to possess the ability to degrade alkanes, phenols, and naphthalene ( Velmurugan and Arunachalam, 2009 ; Wang et al, 2010 , 2014 ). In addition, following the screening of Actinobacteria from a marine lake, a Brachybacterium sp.…”

Section: Resultsmentioning

confidence: 99%

Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium

et al. 2022

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Many marine bacteria produce extracellular enzymes that degrade complex molecules to facilitate their growth in environmental conditions that are often harsh and low in nutrients. Marine bacteria, including those inhabiting sea sponges, have previously been reported to be a promising source of polyesterase enzymes, which have received recent attention due to their potential ability to degrade polyethylene terephthalate (PET) plastic. During the screening of 51 marine bacterial isolates for hydrolytic activities targeting ester and polyester substrates, a Brachybacterium ginsengisoli B129SM11 isolate from the deep-sea sponge Pheronema sp. was identified as a polyesterase producer. Sequence analysis of genomic DNA from strain B129SM11, coupled with a genome “mining” strategy, allowed the identification of potential polyesterases, using a custom database of enzymes that had previously been reported to hydrolyze PET or other synthetic polyesters. This resulted in the identification of a putative PET hydrolase gene, encoding a polyesterase-type enzyme which we named BgP that shared high overall similarity with three well-characterized PET hydrolases—LCC, TfCut2, and Cut190, all of which are key enzymes currently under investigation for the biological recycling of PET. In silico protein analyses and homology protein modeling offered structural and functional insights into BgP, and a detailed comparison with Cut190 revealed highly conserved features with implications for both catalysis and substrate binding. Polyesterase activity was confirmed using an agar-based polycaprolactone (PCL) clearing assay, following heterologous expression of BgP in Escherichia coli. This is the first report of a polyesterase being identified from a deep-sea sponge bacterium such as Brachybacterium ginsengisoli and provides further insights into marine-derived polyesterases, an important family of enzymes for PET plastic hydrolysis. Microorganisms living in association with sponges are likely to have increased exposure to plastics and microplastics given the wide-scale contamination of marine ecosystems with these plastics, and thus they may represent a worthwhile source of enzymes for use in new plastic waste management systems. This study adds to the growing knowledge of microbial polyesterases and endorses further exploration of marine host-associated microorganisms as a potentially valuable source of this family of enzymes for PET plastic hydrolysis.

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Section: Resultsmentioning

confidence: 99%

Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium

et al. 2022

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“…The predominant genera of Actinobacteria in the degradation and bioremediation were reported by Rathore et al (2021). They are Rhodococcus, Nocardiopsis, Streptomycetes, Gordonia, Dietzia, Actinopolyspora, Amycolicicoccus, Micrococcus, Arthrobacter, Salinispora, Micromonospora, Actinomadura, Streptosporangium, Streptomonospora, Prauserella, Pseudonocardia, Aeromicrobium, Marinactinospora, and Microbacterium.…”

Section: Actinobacteriamentioning

confidence: 98%

“…Most actinobacteria are known as bioactive compounds producers, such as anticancer, antimicrobial, antifungal, and others, but recently those microorganisms have also been found to degrade complex polymers (Goodfellow, 1983;Sharma et al, 2014). Besides being known as a producer of many secondary metabolites, the marine actinobacteria are also reported for the degradation of oil spills, chlorobenzenes, aliphatic and polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), phenols, n-alkanes, heavy metal compounds, and plastic materials (Rathore et al, 2021). The actinobacteria are also reported to biodegrade various pollutants, such as pesticides, plastics, rubber, heavy metal, and other organic matters (Diez, 2010;Tseng et al, 2007).…”

Section: Actinobacteriamentioning

confidence: 99%

Microbial Biofilm of Plastic in Tropical Marine Environment and their Potential for Bioremediation of Plastic Waste

Afianti¹,

Rachman²,

Hatmanti³

et al. 2022

J. Ecol. Eng.

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Plastic debris has become a global problem due to its widespread distribution and accumulation in the marine environment. Indigenous bacteria in the marine environment are able to quickly contaminate plastic surface and assemble to form successional plastisphere-specific bacterial. The formation of microbial biofilms on the plastic surface can indirectly initiate the degradation of plastic polymers. The environmental conditions of the tropical region make the growth of microbial biofilms become faster. However, the study on the biodiversity of microorganisms in marine plastic debris is limited to the northern hemisphere, which includes subtropical and temperate regions. This review provides current studies of biodiversity and community structure of plastisphere in tropical environments, including bacteria and microalgae, and their potential to degrade the plastic polymer. A systematic literature search has been conducted using Scopus with different combinations of keywords. In addition, Google Scholar databases were also used to find more studies on some specific topics, including plastic degrading organisms. The climate-associated areas have been grouped according to the latitude of the study site into tropical, subtropical, and temperate latitudes. The microorganisms analyzed in this review are only bacteria, actinobacteria, and microalgae.

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“…Among the marine microorganisms, Actinobacteria members are considered as key members due to their wide distribution and diverse biological functions ( Rathore et al, 2021 ). The Actinobacteria bacteria ubiquitously distribute in marine sediments ( Solano et al, 2009 ), sea water ( Sheikh et al, 2019 ), marine organic aggregates ( Lam, 2006 ), marine sponges ( Gandhimathi et al, 2009 ; Sheikh et al, 2019 ), and deep-sea gas hydrate reservoirs ( Wang et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Notably, Actinobacteria have broad application potentials, such as production of large number of secondary metabolites for developing novel antibiotics ( Wang et al, 2014 ; Kamjam et al, 2017 ); production of variety of enzymes including alkaline protease, xylanase, α-galactosidase ( Temuujin et al, 2016 ; Sanjivkumar et al, 2017 ; Thakrar and Singh, 2019 ). In addition, Actinobacteria could degrade and metabolize foreign compounds such as heavy metals, hydrocarbons, pesticides and plastics, and they are also potential candidates for bioremediation ( Rathore et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Deep-Sea Actinobacterium Strain Uncovers Its Prominent Capability of Utilizing Taurine and Polyvinyl Alcohol

et al. 2022

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Actinobacteria represent a large group of important prokaryotes with great application potentials and widely distribute in diverse natural environments including the ocean. However, compared to their terrestrial cultured members, there are much less available marine Actinobacteria, especially deep-sea counterparts. Here, we cultured a bacterial strain of deep-sea actinobacterium, Marmoricola sp. TYQ2, by using a basal medium supplemented with taurine. Consistently, the growth of strain TYQ2 was significantly promoted by the supplement of taurine. Transcriptomic analysis showed that the expressions of genes encoding proteins associated with taurine metabolization and utilization as well as energy generation were evidently up-regulated when taurine was added. Moreover, strain TYQ2 was demonstrated to degrade polyvinyl alcohol (PVA) with the involvement of the redox cycle of extracellular quinol and quinone and the reduction of iron to ferrous, and strain TYQ2 could utilize the degradation products for energy production, thereby supporting bacterial growth. Overall, our experimental results demonstrate the prominent degradation capabilities of Marmoricola sp. TYQ2 toward the organics taurine and PVA.

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Marine Actinobacteria: New Horizons in Bioremediation

Cited by 11 publications

References 124 publications

Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium

Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium

Microbial Biofilm of Plastic in Tropical Marine Environment and their Potential for Bioremediation of Plastic Waste

Characterization of a Deep-Sea Actinobacterium Strain Uncovers Its Prominent Capability of Utilizing Taurine and Polyvinyl Alcohol

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