Marine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential

Becerril-Espinosa, Amayaly; Freel, Kelle C.; Jensen, Paul R.; Soria-Mercado, Irma E.

doi:10.1007/s10482-012-9863-3

Cited by 36 publications

(25 citation statements)

References 34 publications

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“…According to the revised 16S rRNA gene sequence similarity threshold which is currently 99% for Actinobacteria 47 , representative isolates were clustered into 39 OTUs. In recent studies, 37 and 147 OTUs have been reported from 136 and 358 representative isolates, respectively from terrestrial 14 and marine ecosystems 48 . Relatively high number of OTUs deduced from 66 representative isolates indicates the presence of many novel taxonomical groups.…”

Section: Discussionmentioning

confidence: 99%

Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

Jose

Jha

2017

Sci Rep

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Actinobacteria are the major source of bioactive natural products that find their value in research and drug discovery programmes. Antimicrobial resistance and the resulting high demand for novel antibiotics underscore the need for exploring novel sources of these bacteria endowed with biosynthetic potential. Intertidal ecosystems endure regular periods of immersion and emersion, and represent an untapped source of Actinobacteria. In this study, we studied the diversity and biosynthetic potential of cultivable Actinobacteria from intertidal sediments of Diu Island in the Arabian Sea. A total of 148 Actinobacteria were selectively isolated using a stamping method with eight isolation media. Isolates were grouped into OTUs based on their 16S rRNA gene sequence, and categorized within actinobacterial families such as Glycomycetaceae, Micromonosporaceae, Nocardiaceae, Nocardiopsaceae, Pseudonocardiaceae, Streptomycetaceae, and Thermomonosporaceae. The biosynthetic potential of the Actinobacteria, necessary for secondary metabolite biosynthesis, was screened and confirmed by extensive fingerprinting approaches based on genes coding for polyketide synthases and nonribosomal peptide synthetases. The observed biosynthetic potential was correlated with the antibacterial activity exhibited by these isolates in laboratory conditions. Ultimately, the results demonstrate that intertidal sediment is a rich source of diverse cultivable Actinobacteria with high potential to synthesize novel bioactive compounds in their genomes.

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Section: Discussionmentioning

confidence: 99%

Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

Jose

Jha

2017

Sci Rep

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“…Besides, orange-pigmented colonies without aerial mycelium formation were also obtained from three sampling sites, which belong to the genus Micromonospora. Additional features found on these colonies were blackened colony surface due to the presence of spores (Bredholdt et al 2007;Becerril-Espinosa et al 2013).…”

Section: Culturable Actinobacteriamentioning

confidence: 98%

Actinobacteria from Greenwich Island and Dee Island: Isolation, diversity and distribution

Lim

Hoon

Cheah

2017

lsmb

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Actinobacteria from underexplored and unusual environments have gained significant attention for their capability in producing novel bioactive molecules of diverse chemical entities. Streptomyces is the most prolific Actinobacteria in producing useful molecules. Rapid decline effectiveness of existing antibiotics in the treatment of infections are caused by the emergence of multidrug-resistant pathogens. Intensive efforts are urgently required in isolating non-Streptomyces or rare Actinobacteria and understanding of their distribution in the harsh environment for new drug discovery. In this study, pretreatment of soil samples with 1.5% phenol was used for the selective isolation of Actinobacteria from Dee Island and Greenwich Island. A high number of non-Streptomyces (69.4%) or rare Actinobacteria was significantly recovered despite the Streptomyces (30.6%), including the genera Micromonospora, Micrococcus, Kocuria, Dermacoccus, Brachybacterium, Brevibacterium, Rhodococcus, Microbacterium and Rothia. Reduced diversity and shift of distribution were observed at the elevated level of soil pH. The members of genera Streptomyces, Micromonospora and Micrococcus were found to distribute and tolerate to a relatively high pH level of soil (pH 9.4-9.5), and could potentially be alkaliphilic Actinobacteria. The phylogenetic analysis had revealed some potentially new taxa members of the genera Micromonospora, Micrococcus and Rhodococcus. Principal Component Analysis of soil samples was used to uncover the factors that underlie the diversity of culturable Actinobacteria. Water availability in soil was examined as the principal factor that shaped the diversity of the Actinobacteria, by providing a dynamic source for microbial interactions and elevated diversity of Actinobacteria.

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“…There are many studies describing the diversity of culturable Actinobacteria from marine sediments [22][23][24], as well as those recovered from marine invertebrates, including ascidians and sponges [14,25]. Indeed, all these previous studies pointed out the genera found in Rocas Atoll among the most common ones, especially in studies guided by cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Marine Bacteria from Rocas Atoll as a Rich Source of Pharmacologically Active Compounds

et al. 2019

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Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts. About one third (34%) of these extracts were recognized as cytotoxic against human colon adenocarcinoma HCT-116 cell line. 16S rRNA gene sequencing analyses revealed that the bacteria producing cytotoxic extracts were mainly from the Actinobacteria phylum, including Streptomyces, Salinispora, Nocardiopsis, and Brevibacterium genera, and in a smaller proportion from Firmicutes phylum (Bacillus). The search in the spectral library in GNPS (Global Natural Products Social Molecular Networking) unveiled a high chemodiversity being produced by these bacteria, including rifamycins, antimycins, desferrioxamines, ferrioxamines, surfactins, surugamides, staurosporines, and saliniketals, along with several unidentified compounds. Using an original approach, molecular networking successfully highlighted groups of compounds responsible for the cytotoxicity of crude extracts. Application of DEREPLICATOR+ (GNPS) allowed the annotation of macrolide novonestimycin derivatives as the cytotoxic compounds existing in the extracts produced by Streptomyces BRB-298 and BRB-302. Overall, these results highlighted the pharmacological potential of bacteria from this singular atoll.

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Marine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential

Cited by 36 publications

References 34 publications

Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

Actinobacteria from Greenwich Island and Dee Island: Isolation, diversity and distribution

Marine Bacteria from Rocas Atoll as a Rich Source of Pharmacologically Active Compounds

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