Maribacter litopenaei sp. nov., isolated from the intestinal tract of the Pacific white shrimp Litopenaeus vannamei

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated HL-LV01T, was isolated from the intestinal tract content of the Pacific white shrimp Litopenaeus vannamei. The 16S rRNA gene sequence of strain HL-LV01T showed that the strain was clearly a member of the genus Maribacter . According to the phylogenetic analyses, strain HL-LV01T was most closely related to the species Maribacter flavus KCTC 42508T with 98.2 % sequence similarity. The average nucleotide identity and digital DNA–DNA hybridization values between strain HL-LV01T and M. flavus KCTC 42508T were 80.6 % and 23.0 %, respectively, indicating different genomic species in the genus Maribacter . Strain HL-LV01T showed optimal growth at 35 °C, pH 7.0, and 2.5 % (w/v) sea salts. The major cellular fatty acids were iso-C15 : 0 (32.5 %), iso-C17 : 0 3-OH (22.3 %), and iso-C15 : 1 G (15.5 %). The major respiratory quinone was menaquinone-6. The polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids, and seven unidentified lipids. The genomic DNA G+C content of the strain was 39.8 mol%. The comprehensive phylogenetic, genomic, phenotypic, and chemotaxonomic results indicate that strain HL-LV01T is distinct from validly published species of the genus Maribacter . Hence, we propose strain HL-LV01T as a novel species belonging to the genus Maribacter , for which the name Maribacter litopenaei sp. nov. is proposed. The type strain is HL-LV01T (= KCCM 90498T = JCM 35709T).

Maribacter halichondriae sp. nov., isolated from the marine sponge Halichondria panicea, displays features of a sponge-associated life style

“…Since there are only two publications available, which include information on genome analysis of a Maribacter sp. type strains (Hu et al 2015b ; Kim et al 2023 ), we displayed genome-derived features of strain Hal144 T in comparison to the next related type strains in Table 2 . Genomic information of strain Hal144 T support differentiation from the five closely related Maribacter spp.…”

“…oxygen-regulating Crp/Fnr proteins, heat and cold shock proteins, and two systems playing a role in heavy-metal resistance, resistance-nodulation-cell division (RND) proteins and a lead, cadmium, zinc, and mercury transporting ATPase. A second known genome analysis was carried out for Maribacter litopenaei HL-LV01 T (Kim et al 2023 ). This motile strain encoded gliding motility-associated proteins, in contrast to the non-motile strain Hal144 T .…”