Marek's Disease Virus Protein Kinase Gene Identified within the Short Unique Region of the Viral Genome Is Not Essential for Viral Replication in Cell Culture and Vaccine-Induced Immunity in Chickens

Characterization of proteins encoded by the short unique region of herpesvirus of turkeys byin vitroexpression

“…Similar discrepancies between the predicted and observed Mr were reported for PK encoded by the U s of HSV-1 (Frame et al, 1987). Sakaguchi et al (1993) reported that polyclonal serum directed against MDV PK expressed in baculovirus identified two forms of PK (M r 44K and 45K, respectively) in MDV:infected cells. However, the antiserum used cross-reacted strongly with some host protein(s).…”

“…However, the antiserum used cross-reacted strongly with some host protein(s). It is possible that detection of the processed isoform of MDV PK (M r 53K) described here was masked in the previous study (Sakaguchi et al, 1993). The mobility of HVT PK, as that of HVT US1, was not affected after treatment of in vitro translation products with phosphatases (data not shown).…”

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

“…The MD vaccine viruses are considered one of the most potent vectors for polyvalent live vaccines expressing foreign antigens related to vaccineinduced immunity against poultry diseases for the following reasons. (i) The viruses induce lifetime protection against MD with just one vaccination (39), (ii) the viruses have a natural host range limited to avian species, and therefore, the vectors would be safe for other domestic animals and people working in the poultry industry, and (iii) techniques for generating recombinant MDVs have been well established (45,49). Among the vaccine viruses, HVT has been used worldwide both as live vaccine and polyvalent vaccine vector (13,17,28,29,41,42,53).…”

“…Congr., p. [150][151][152][153][154][155]1992). Of these sites, those of the US3 and US10 genes and the junction region between the unique short (U S ) and short inverted repeats were nonessential not only for viral growth in culture but also for vaccineinduced immunity (45,49,54). In addition, other groups reported several nonessential sites within U S repeat for viral growth in culture (9,37,38).…”

Structure and properties of a herpesvirus of turkeys recombinant in which US1, US10 and SORF3 genes have been replaced by a lacZ expression cassette

“…It is not known whether HVT PK is required for virus replication and whether attempts to generate PK deletion mutants might favour non-homologous recombination. However, this is unlikely since there is evidence that the PK gene of MDV is non-essential (Sakaguchi et al, 1993). PCR analysis of virus produced from individual plaques after the second and subsequent rounds of screening suggested that the recombinant had been generated at an early stage in the selection process.…”