Marek’s disease virus as a CRISPR/Cas9 delivery system to defend against avian leukosis virus infection in chickens

Liu, Yongzhen; Xu, Zengkun; Zhang, Yu; Yu, Mengmeng; Wang, Suyan; Gao, Yulong; Liu, Changjun; Zhang, Yanping; Gao, Li; Qi, Xiaole; Cui, Hongyu; Pan, Qing; Li, Kai

doi:10.1016/j.vetmic.2020.108589

Cited by 10 publications

(9 citation statements)

References 40 publications

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“…Poultry is highly susceptible to viruses, such as MDV (Mark's disease virus), the highly pathogenic ALV-J (Avian leukosis virus J) [72], and AIV (avian influenza virus) [73], which all have fast transmission speeds and high mortality rates. Koslova et al successfully bred chNHE1-KO homozygous mutant chickens resistant to ALV infection using CRISPR-Cas9 [74].…”

Section: Application Of Crispr-cas9 Technology In Chicken Genetic Bre...mentioning

confidence: 99%

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Lu,

Zhang,

et al. 2024

Agriculture

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Due to recent innovations in gene editing technology, great progress has been made in livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other livestock. Gene-editing technology involves knocking in, knocking out, deleting, inhibiting, activating, or replacing specific bases of DNA or RNA sequences at the genome level for accurate modification, and such processes can edit genes at a fixed point without needing DNA templates. In recent years, although clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene-editing technology has been widely used in research into the genetic breeding of animals, the system’s efficiency at inserting foreign genes is not high enough, and there are certain off-target effects; thus, it is not appropriate for use in the genome editing of large livestock such as cashmere goats. In this study, the development status, associated challenges, application prospects, and future prospects of CRISPR/Cas9-mediated precision gene-editing technology for use in livestock breeding were reviewed to provide a theoretical reference for livestock gene function analysis, genetic improvement, and livestock breeding that account for characteristics of local economies.

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Section: Application Of Crispr-cas9 Technology In Chicken Genetic Bre...mentioning

confidence: 99%

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Lu,

Zhang,

et al. 2024

Agriculture

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“…Though the knockdowns observed were relatively small, with an average reduction of around 20 -25% per construct, this suggests that miRNA linked shRNA expression could be further developed for RNAi based viral targeting applications in vivo, such as by linking shRNA expression to expression of innate immune response genes both in vitro and in vivo (Challagulla et al, 2022). As a more specific alternative to RNAi-based knockdown, several studies have developed CRISPR/Cas-based systems for direct viral genome targeting in avian cells (K. Liu et al, 2020;Challagulla, Jenkins, et al, 2021). To ensure greater antiviral efficacy, these systems commonly target multiple loci within genes key to viral replication, as these genes are more functionally conserved and thus less likely to develop escape mutations which could inhibit effective viral targeting.…”

Section: Using Shrnas Cas9 and Cas13 To Directly Target Pathogensmentioning

confidence: 99%

“…These include the Cas12 effectors with both DNA and RNA targeting activity or the exclusively RNA targeting Cas13 effector proteins ( Zetsche et al, 2015;Abudayyeh et al, 2017). A similarly wide variety of systems have been utilised for delivery and expression of the CRISPR system in avian models, including transposon vectors, plasmid vectors or modified viral vectors such as adenovirus or Marek's Disease Virus (MDV) (Abu-Bonsrah et al, 2016;Lee et al, 2019;Liu et al, 2020). To generate CRISPR based homozygous edited in vivo knockout models, these systems are most commonly used to create transgenic PGC cultures, which are then reinserted into host embryos as described above and crossbred to produce homozygous transgenic offspring (Figure 1).…”

mentioning

confidence: 99%

Chicken genome editing for investigating poultry pathogens

2022

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Major advances in pathogen identification, treatment, vaccine development, and avian immunology have enabled the enormous expansion in global poultry production over the last 50 years. Looking forward, climate change, reduced feed, reduced water access, new avian pathogens and restrictions on the use of antimicrobials threaten to hamper further gains in poultry productivity and health. The development of novel in vitro cell culture systems coupled with new genetic tools to investigate gene function will aid in developing novel interventions for existing and newly emerging poultry pathogens. Our growing capacity to cryopreserve and generate genome-edited chicken lines will also be useful for developing improved chicken breeds for poultry farmers and conserving chicken genetic resources. IntroductionOf the three largest animal protein sources, poultry, beef, and pork, poultry is the most affordable, has the shortest production cycle, and has the least environmental impact. Ninety percent of the world's poultry meat production comes from chicken which provides both meat and eggs for consumers of all socioeconomic strata (FAO, 2021). In 2020, global poultry production reached over 70 billion chicken, producing 1.6 trillion eggs and 133.3 million tonnes of poultry meat (FAO, 2022). To ensure flocks are protected against disease, proper rearing of healthy chickens requires a stringent prescription of vaccinations, high flock biosecurity, and the precise application of anti-bacterials or anti-protozoics. However, in commercial farming systems, increasing flock density and increased global climatic impacts are expected to create future stresses for poultry production leading to increased incidences of infections (Mottet & Tempio, 2017). Additionally, in many parts of the world, poultry is raised in open production systems or in small village farms where they have a higher exposure to pathogens, reduced biosecurity, reduced access to pharmaceuticals, and a lower number of vaccinations due to reduced access to vaccines (Cristalli & Capua, 2007). Advances in genetic modification technologies and next-generation sequencing serve as new tools to identify genes activated in both hosts and pathogens during infection and modify

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“…Other herpesvirus vaccine vectors such as duck enteritis virus (DEV) 133,134 and ILTV 135 have also been developed via NHEJ-CRISPR-Cas9 and Cre-Lox Systems. Recombinant MDV vector carrying all the CRISPR-Cas9 components using an attenuated MDV vaccine strain (814 strain) for in vivo editing of reticuloendotheliosis virus (REV) and avian leukosis virus subgroup J (ALV-J) infection have recently been reported, 136,137 providing efficient delivery of CRISPR components into chickens, inducing a drastic reduction of REV and ALV-J viral load, significantly diminishing clinical disease.…”

Section: Herpesvirusesmentioning

confidence: 99%

Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines

et al. 2021

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CRISPR-Cas technology, discovered originally as a bacterial defense system, has been extensively repurposed as a powerful tool for genome editing for multiple applications in biology. In the field of virology, CRISPR-Cas9 technology has been widely applied on genetic recombination and engineering of genomes of various viruses to ask some fundamental questions of virus-host interactions. Its high efficiency, specificity, versatility, and low cost have also provided great inspirations and hope in the field of vaccinology to solve a series of bottleneck problems in the development of recombinant viral vaccines. This review highlights the applications of CRISPR editing in the technological advances compared to the traditional approaches used for the construction of recombinant viral vaccines and vectors, the main factors affecting their application, and the challenges that need to be overcome for further streamlining their effective usage in the prevention and control of diseases. Factors affecting efficiency, target specificity and fidelity of CRISPR-Cas editing in the context of viral genome editing and development of recombinant vaccines are also discussed.

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Marek’s disease virus as a CRISPR/Cas9 delivery system to defend against avian leukosis virus infection in chickens

Cited by 10 publications

References 40 publications

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Chicken genome editing for investigating poultry pathogens

Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines

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