Marble-burying behavior test as a murine model of compulsive-like behavior

Dixit, Pankaj; Sahu, Rohit; Mishra, Dinesh Kumar

doi:10.1016/j.vascn.2020.106676

Cited by 50 publications

(38 citation statements)

References 90 publications

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“…Kp-8 has also significantly reduced the number and time of goal-oriented interaction with marbles in the MB. Although MB has long been considered a test for anxiety-like behavior, now several authors have expressed doubts about it [73]. According to Thomas, the number of buried marbles does not correlate with other anxiety-like traits, namely central time in the open field test and light-dark transitions in the light-dark box test [74].…”

Section: Discussionmentioning

confidence: 99%

“…According to Thomas, the number of buried marbles does not correlate with other anxiety-like traits, namely central time in the open field test and light-dark transitions in the light-dark box test [74]. The utility of the test as a screening tool for anxiety has also been questioned based on the findings that most anxiolytics and antidepressant drugs reduce marble burying behavior secondary to drug-induced hypolocomotion [73]. It has been suggested that digging and burying are species-specific, innate behavioral patterns that are likely triggered by an exploratory drive [75] or by the bedding itself [76].…”

Section: Discussionmentioning

confidence: 99%

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Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats

et al. 2021

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Kisspeptins (Kp) are RF-amide neuropeptide regulators of the reproductive axis that also influence anxiety, locomotion, and metabolism. We aimed to investigate the effects of intracerebroventricular Kp-8 (an N-terminally truncated octapeptide) treatment in Wistar rats. Elevated plus maze (EPM), computerized open field (OF), and marble burying (MB) tests were performed for the assessment of behavior. Serum LH and corticosterone levels were determined to assess kisspeptin1 receptor (Kiss1r) activation and hypothalamic-pituitary-adrenal axis (HPA) stimulation, respectively. GABA release from the nucleus accumbens (NAc) and dopamine release from the ventral tegmental area (VTA) and NAc were measured via ex vivo superfusion. Kp-8 decreased open arm time and entries in EPM, and also raised corticosterone concentration, pointing to an anxiogenic effect. Moreover, the decrease in arm entries in EPM, the delayed increase in immobility accompanied by reduced ambulatory activity in OF, and the reduction in interactions with marbles show that Kp-8 suppressed exploratory and spontaneous locomotion. The increase in GABA release from the NAc might be in the background of hypolocomotion by inhibiting the VTA-NAc dopaminergic circuitry. As Kp-8 raised LH concentration, it could activate Kiss1r and stimulate the reproductive axis. As Kiss1r is associated with hyperlocomotion, it is more likely that neuropeptide FF receptor activation is involved in the suppression of locomotor activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats

et al. 2021

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“…By introducing new interaction partners, the test can be modified for social memory features [81]. Impairments of daily life activities can simply be tested by assessing nest building, i.e., scoring the complexity of the nest built from a paper tissue within 24-48 h. Disturbed nest building is observed in several neuropsychiatric disorders models, including obsessive compulsive disorders (OCDs) [82,94]. Widely used, but critically discussed, is the use of the Porsolt forced swim test in depression models.…”

Section: Depressionmentioning

confidence: 99%

Probing the Skin–Brain Axis: New Vistas Using Mouse Models

Weiglein

Gaffal

Albrecht

2022

IJMS

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Inflammatory diseases of the skin, including atopic dermatitis and psoriasis, have gained increasing attention with rising incidences in developed countries over the past decades. While bodily properties, such as immunological responses of the skin, have been described in some detail, interactions with the brain via different routes are less well studied. The suggested routes of the skin–brain axis comprise the immune system, HPA axis, and the peripheral and central nervous system, including microglia responses and structural changes. They provide starting points to investigate the molecular mechanisms of neuropsychiatric comorbidities in AD and psoriasis. To this end, mouse models exist for AD and psoriasis that could be tested for relevant behavioral entities. In this review, we provide an overview of the current mouse models and assays. By combining an extensive behavioral characterization and state-of-the-art genetic interventions with the investigation of underlying molecular pathways, insights into the mechanisms of the skin–brain axis in inflammatory cutaneous diseases are examined, which will spark further research in humans and drive the development of novel therapeutic strategies.

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“…The phospholipid content of the BMP substrate and phospholipid-enriched fractions were measured by quantitative 31 P-NMR spectroscopy using a Bruker Avance III HD 500 MHz unit (Bruker, Massachusetts, USA) and carried out by Spectral Services (Köln, Germany). To concentrate the phospholipid content, protein and carbohydrate were removed by enzyme hydrolysis and ultrafiltration, as previously described [20,21] with adaptations detailed in the supplementary data. Digestive enzymes Alcalase 2.4 L (a serine endoproteinase from Bacillus licheniformis) and Flavourzyme 500 L (a blend of endoproteinase and exopep-tidase from Aspergillus oryzae) were obtained from Novozymes (Bagsvaerd, Denmark).…”

Section: Phospholipid Enrichment Of Buttermilk Powder Substrate Into Dietsmentioning

confidence: 99%

“…At day 26 (Figure 1B), the marble burying test was performed as previously described [27] to assess neophobia, an anxiety-like behavior to new objects. [20]…”

Section: Marble Burying Testmentioning

confidence: 99%

Dietary Milk Phospholipids Attenuate Chronic Stress‐Induced Changes in Behavior and Endocrine Responses across the Lifespan

Schverer

Donoso

Mitchell

et al. 2021

Molecular Nutrition Food Res

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Scope: Increasing scientific evidence is validating the use of dietary strategies to support and improve brain health throughout the lifespan, with tailored nutritional interventions catering for specific life stages. Dietary phospholipid supplementations in early life and adulthood are shown to alleviate some of the behavioral consequences associated with chronic stress. This study aims to explore the protective effects of a tailored phospholipid-enriched buttermilk on behavioral and endocrine responses induced by chronic psychosocial stress in adulthood, and to compare these effects according to the life stage at which the supplementation is started. Methods and Results: A novel developed phospholipid-enriched dairy product is assessed for its effects on social, anxiety-and depressive-like behaviors, as well as the stress response and cognitive performance following chronic psychosocial stress in C57BL/6J mice, with supplementation beginning in adulthood or early life. Milk phospholipid supplementation from birth protects adult mice against chronic stress-induced changes in endocrine response to a subsequent acute stressor and reduces innate anxiety-like behavior in non-stressed animals. When starting in adulthood, the dietary intervention reverses the anxiety-like phenotype caused by chronic stress exposure. Conclusion: Dairy-derived phospholipids exert differential protective effects against chronic psychosocial stress depending on the targeted life stage and duration of the dietary supplementation.

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Marble-burying behavior test as a murine model of compulsive-like behavior

Cited by 50 publications

References 90 publications

Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats

Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats

Probing the Skin–Brain Axis: New Vistas Using Mouse Models

Dietary Milk Phospholipids Attenuate Chronic Stress‐Induced Changes in Behavior and Endocrine Responses across the Lifespan

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