Maradolipids: Diacyltrehalose Glycolipids Specific to Dauer Larva in <i>Caenorhabditis elegans</i>

Penkov, Sider; Mende, Fanny; Zagoriy, Vyacheslav; Erkut, Cihan; Martin, René; Pässler, Ulrike; Schuhmann, Kai; Schwudke, Dominik; Грүнер, Маргит; Mäntler, Jana; Reichert-Müller, Thomas; Shevchenko, Andrej; Knölker, Hans‐Joachim; Kurzchalia, Teymuras V.

doi:10.1002/ange.201004466

Cited by 8 publications

(15 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All single mutants were outcrossed at least three times. Compound mutant and transgenic strains were produced during this study or past studies as published 15,16 or described below. daf-9(dh6);dhEx24 and daf-9(dh6);daf-12(rh61rh411) were provided by the group of Dr A. Antebi.…”

Section: Discussionmentioning

confidence: 99%

“…N2 and daf-16(mu86) were grown on cholesterol or lophenolcontaining plates to obtain L3 larvae or dauer/dauer-like larvae, respectively 25 . Total RNA was isolated using the PureLink RNA Mini Kit (Ambion) and equal amounts of all samples were reverse transcribed with SuperScript III (Invitrogen) using oligo(dT) [12][13][14][15][16][17][18] primers. cDNA quality was tested using standard PCR.…”

Section: Discussionmentioning

confidence: 99%

“…The DDtps dauers, however, had proper dauer morphology as examined using electron microscopy 16 and were resistant to SDS treatment. To investigate the dauer formation in DDtps under controlled conditions, we tested dauer formation in the presence of synthetic dauer-inducing pheromones.…”

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

“…Recently, we showed that dauer larvae produce stagespecific glycolipids (maradolipids) containing the disaccharide trehalose 16 . Here we exploited a C. elegans strain that was unable to synthesize trehalose to investigate whether trehalose production could also be involved in the determination of dauer fate.…”

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

“…Next, the phosphate is removed by trehalose-6-phosphate phosphatase. We produced a mutant C. elegans strain that lacked functional TPS-1 and TPS-2 proteins (DDtps) and consequently was unable to synthesize trehalose 16 .…”

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

See 4 more Smart Citations

Integration of carbohydrate metabolism and redox state controls dauer larva formation in Caenorhabditis elegans

et al. 2015

Self Cite

View full text Add to dashboard Cite

Under adverse conditions, Caenorhabditis elegans enters a diapause stage called the dauer larva. External cues signal the nuclear hormone receptor DAF-12, the activity of which is regulated by its ligands: dafachronic acids (DAs). DAs are synthesized from cholesterol, with the last synthesis step requiring NADPH, and their absence stimulates dauer formation. Here we show that NADPH levels determine dauer formation in a regulatory mechanism involving key carbohydrate and redox metabolic enzymes. Elevated trehalose biosynthesis diverts glucose-6-phosphate from the pentose phosphate pathway, which is the major source of cellular NADPH. This enhances dauer formation due to the decrease in the DA level. Moreover, DAF-12, in cooperation with DAF-16/FoxO, induces negative feedback of DA synthesis via activation of the trehalose-producing enzymes TPS-1/2 and inhibition of the NADPH-producing enzyme IDH-1. Thus, the dauer developmental decision is controlled by integration of the metabolic flux of carbohydrates and cellular redox potential.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

Section: Trehalose-deficient Mutants Exhibit Reduced Dauer Formationmentioning

confidence: 99%

See 3 more Smart Citations

Integration of carbohydrate metabolism and redox state controls dauer larva formation in Caenorhabditis elegans

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Additive‐Free Gold(III)‐Catalyzed Stereoselective Synthesis of 2‐Deoxyglycosides Using Phenylpropiolate Glycosides as Donors

Shaw

Kumar

2019

Chemistry An Asian Journal

View full text Add to dashboard Cite

Stereoselective synthesis of deoxyglycosides has been achieved from benchtop stable and easily synthesizable deoxy-phenylpropiolate glycosides (D-PPGs) using gold(-III) salt as catalyst under externala dditive-free conditions. Under asimple catalytic system,D-PPGs reacted with avariety of sugar and non-sugar acceptors to produce majorly astereoselective O/N-deoxyglycosides in good to excellent yields and regenerate easily separable and reusable phenylpropiolic acid. Deoxy-PPGs containing armed and disarmed groups survived well under the optimized reaction conditions. In addition, the orthogonaln ature of D-PPGs was showcased, and 1,1'-linked trehalose-types ugar was also synthesized.[a] M.

show abstract

Long‐Chain Lipids Are Required for the Innate Immune Recognition of Trehalose Diesters by Macrophages

et al. 2011

View full text Add to dashboard Cite

show abstract

Maradolipids: Diacyltrehalose Glycolipids Specific to Dauer Larva in Caenorhabditis elegans

Cited by 8 publications

References 18 publications

Integration of carbohydrate metabolism and redox state controls dauer larva formation in Caenorhabditis elegans

Integration of carbohydrate metabolism and redox state controls dauer larva formation in Caenorhabditis elegans

Additive‐Free Gold(III)‐Catalyzed Stereoselective Synthesis of 2‐Deoxyglycosides Using Phenylpropiolate Glycosides as Donors

Long‐Chain Lipids Are Required for the Innate Immune Recognition of Trehalose Diesters by Macrophages

Contact Info

Product

Resources

About