Abstract:We are developing a linkage map of tetraploid alfalfa (Medicago sativa L.) based on restriction fragment length polymorphism (RFLP) in order to locate genes associated with forage yield, which may facilitate selection and cultivar improvement. Two highly diverse, heterozygous genotypes, WISF AL-6 (M sativa subsp.falcata) and ABI408 (M sativa subsp. sativa), were hand-crossed with emasculation to produce an F 1 population of 200 individuals. The population was planted in the field at two Iowa locations in 1998 … Show more
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