Mapping the stripe rust resistance gene in Chinese wheat Guinong 775

Abstract

Wheat stripe rust, caused by Puccinia striiformis Eriks. f. sp. tritici, is a destructive wheat disease worldwide. To find new sources of resistance, Guinong 775 wheat was previously bred and identified as having a strong resistance against stripe rust, powdery mild and leaf rust. Guinong 775 showed a strong resistance against mixed fungi (CYR32 and CH42) associated with stripe rust that are common in Guiyang and Qianxinan. It was crossed with the susceptible wheat line Huixianhong to develop F1, F2 and F3 hybrids. In this study, 173 simple sequence repeat markers were used to map the stripe rust resistance gene in Guinong 775, and the resistance phenotype of Guinong 775 was regulated by a single dominant gene named YrGn775. YrGn775 was mapped to chromosome 1BS with the marker Xgwm413 flanking the gene at a distance of 12.0 cM. Genetic and molecular marker analyses demonstrated that this dominant gene may have originated in Aegilops ventricosa. Thus YrGn775 may be a novel gene in wheat.

Genome-wide probing of NBS-LRR encoding genes in red clover (Trifolium pratense L) for the identification of resistance gene analogs in Trifolium alexandrinum L

“…Sequence analysis of RGAs from different plants characterized to contribute to plant defense mech-anisms showed significant homology in specific regions, which latterly are known as conserved domains [4][5][6]. Resistance genes encoding proteins contain conserved domains, i.e., leucine-rich repeats (LRR) and nucleotide-binding site (NBS) domain, and their putative role had been demonstrated in plant disease resistance [7,8].…”