Mapping the role of aromatic amino acids within a blue-light sensing LOV domain

Ding, Yong; Zhao, Ziyue; Matysik, Jörg; Gärtner, Wolfgang; Losi, Aba

doi:10.1039/d1cp02217b

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…The former either sensitizes 1 O 2 [23,107] or decays to form ZnPPIX •+ which can also cause oxidative reactions [21,23]. Either process can prompt oxidation of protein residues [21,23,108]. Since Trp is one of the major targets of photooxidation [23,59,81,109], FTAS supports our interpretation that laser irradiation of ZnPPIX bound to BLG leads to the conversion of Trp to NFK or Kyn.…”

Section: Znppix:blg At Phsupporting

confidence: 78%

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Albalawi,

Castillo,

Denton

et al. 2023

Physchem

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Photosensitization of proteins mediated by chromophores is a mechanism commonly employed by nature and mimicked in a broad array of laboratory research and applications. Nature has evolved specialized complexes of proteins and photosensitizers (PS) that assemble to form photoreceptor proteins (PRP). These are used by many organisms in diverse processes, such as energy conversion, protection against photodamage, etc. The same concept has been used in laboratory settings for many applications, such as the stimulation of neurons or the selective depletion of proteins in a signaling pathway. A key issue in laboratory settings has been the relationship between the photooxidation of proteins and conformational changes in host proteins. For several years, we have been interested in creating non-native PRP using porphyrin PS. In this study, we investigated the self-assembled complex between zinc protoporphyrin IX (ZnPPIX) and bovine β-lactoglobulin (BLG) as a model of non-native PRP. Since BLG undergoes a significant conformational transition near physiological pH, the study was carried out at acidic (pH 5) and alkaline (pH 9) conditions where the two conformations are respectively prevalent. We employed a series of steady-state and time-resolved optical spectroscopies as well as gel electrophoresis to experimentally characterize the photosensitization mechanisms and their effect on the host protein. Our results show that ZnPPIX prompts light-dependent modifications of BLG, which appear to be much more significant at alkaline pH. The modifications seem to be driven by photooxidation of amino acid residues that do not lead to the formation of cross-links or protein fragmentation.

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Section: Znppix:blg At Phsupporting

confidence: 78%

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Albalawi,

Castillo,

Denton

et al. 2023

Physchem

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“…Phototropin regulates phototropism, participates in the phototaxis of chloroplasts, and contributes to the stomatal opening process. 516,543,544 Another example is the protein rhodopsin, which is found in rod cells of the retina and plays a vital role in regulating visual phototransduction by absorbing green-blue light (∼500 nm) through the chromophore 11-cis-retinal. 315 Recently, flavoproteins called cryptochromes have received attention.…”

Section: Light-induced Electron Transfer Processes In Biological Systemsmentioning

confidence: 99%

“…Light-induced ET processes are prominent in specific sensory proteins ,,, and provide another example of a multiscale phenomenon, as illustrated in Figure . Indeed, a link between quantum mechanics (light absorption) and large-scale protein function spans many spatial and temporal scales (see Figure ).…”

Section: Case Studies Of Multiscale Phenomenamentioning

confidence: 99%

“…The Problem : Light-induced electron transfer (ET) processes play a crucial role in biological systems, as these processes often provide the core mechanisms for sensory protein activation, − energy harvesting, , and magnetic field sensing. − ET processes are also involved in detrimental phenomena, including the generation of hydrogen peroxide within cells . A precise understanding of the ET processes poses a significant challenge for modern biophysics.…”

Section: Case Studies Of Multiscale Phenomenamentioning

confidence: 99%

“…For instance, the light-receptor phototropin, present in plants like Avena sativa (oat), contains the blue-light chromophore flavin mononucleotide (FMN) within the LOV domain (light–oxygen–voltage). Phototropin regulates phototropism, participates in the phototaxis of chloroplasts, and contributes to the stomatal opening process. ,, Another example is the protein rhodopsin, which is found in rod cells of the retina and plays a vital role in regulating visual phototransduction by absorbing green-blue light (∼500 nm) through the chromophore 11- cis -retinal . Recently, flavoproteins called cryptochromes have received attention. ,− Cryptochromes are involved in several biological processes, including circadian rhythms, sensing of magnetic fields, − phototropism, and light capture in general, mediated by their chromophore flavin adenine dinucleotide (FAD) …”

Section: Case Studies Of Multiscale Phenomenamentioning

confidence: 99%

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Condensed Matter Systems Exposed to Radiation: Multiscale Theory, Simulations, and Experiment

Solov’yov,

Verkhovtsev,

Mason

et al. 2024

Chem. Rev.

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This roadmap reviews the new, highly interdisciplinary research field studying the behavior of condensed matter systems exposed to radiation. The Review highlights several recent advances in the field and provides a roadmap for the development of the field over the next decade. Condensed matter systems exposed to radiation can be inorganic, organic, or biological, finite or infinite, composed of different molecular species or materials, exist in different phases, and operate under different thermodynamic conditions. Many of the key phenomena related to the behavior of irradiated systems are very similar and can be understood based on the same fundamental theoretical principles and computational approaches. The multiscale nature of such phenomena requires the quantitative description of the radiation-induced effects occurring at different spatial and temporal scales, ranging from the atomic to the macroscopic, and the interlinks between such descriptions. The multiscale nature of the effects and the similarity of their manifestation in systems of different origins necessarily bring together different disciplines, such as physics, chemistry, biology, materials science, nanoscience, and biomedical research, demonstrating the numerous interlinks and commonalities between them. This research field is highly relevant to many novel and emerging technologies and medical applications.

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Catalytic Transformation of Positional Nitrophenol Isomers on Highly Active Ag/Linde‐Type A4 Zeolite: A Comparative Analysis

et al. 2023

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The catalytic transformation of ortho-(o-NP), meta-(m-NP) and para-nitrophenols (p-NP) by Ag nanoparticles (Ag-NPs) stabilised on zeolite A4 (ZA4) is presented for the first time. The catalysts are prepared by microwave-assisted synthesis (Ag/ZA4-MW) or ion exchange (Ag/ZA4-IE). The results indicate that the nitrophenol reduction rate depends on the molecule structure and the presence of low-coordinated metallic Ag-NPs (~2 nm and sub nanometric species). Meanwhile, the reactivity tendency depends on the synthesis methodology (m-NP > p-NP > o-NP and o-NP > m-NP > p-NPfor Ag/ZA4-MW and Ag/ZA4-IE, respectively). The turnover frequency (TOF) values estimated for nitrophenols reduction are superior for Ag/ZA4-MW catalyst by several orders of magnitude compared with those obtained for Ag/ZA4-IE catalyst. In addition, the catalyst exhibits remarkable catalytic stability for the reduction of p-NP even after the fifth consecutive catalytic run. The presently prepared catalysts are characterised by superior catalytic activity for the reduction of nitroaromatic compounds than similar Ag-based materials reported in the literature.

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Mapping the role of aromatic amino acids within a blue-light sensing LOV domain

Abstract: Photosensing LOV (Light, Oxygen, Voltage) domains detect and respond to UVA/Blue (BL) light by forming a covalent adduct between the flavin chromophore and a nearby cysteine, via the decay of...

Cited by 8 publications

References 44 publications

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Condensed Matter Systems Exposed to Radiation: Multiscale Theory, Simulations, and Experiment

Catalytic Transformation of Positional Nitrophenol Isomers on Highly Active Ag/Linde‐Type A4 Zeolite: A Comparative Analysis

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