Mapping the Mouse Cell Atlas by Microwell-Seq

Abstract

It has come to our attention that in preparing the final version of this paper, we inadvertently misspelled the first name of an author Ziming Zhou as ''Zimin Zhou''. In addition, we have made two errors in describing the reagents in the STAR Methods. First, under the subheading of ''Synthesis of barcoded beads'' in the Method Details section, the supplier of the magnetic beads coated with carboxyl groups should be Suzhou Knowledge & Benefit Sphere Tech. Co., Ltd. (diameter 20-25 mm, http://www.kbspheretech. com/), instead of Zhiyi. Second, under the subheading of ''Cell collection and lysis'' in the Method Details section, the concentration of Tris-HCL for the cold lysis buffer should be 0.1 M, instead of 1 M. These errors have been corrected online, and we apologize for any confusions we may have caused.

Decoding the transcriptional response to ischemic stroke in young and aged mouse brain

“…Interestingly, granulocyte-enriched genes (neutrophils, mast cells/basophils) were significantly overrepresented among "more up MCAO18" DE genes (p ≤ 1.67e-6), as were monocyte-enriched genes (p = 2.14e-6) and, to a smaller degree, innate lymphocyte (NK cells; p = 0.025) and activated T-cell-enriched genes (p = 0.003), although T-cell pan markers were barely detectable in our sequencing data ( Figure S9). These trends were recapitulated by expression of individual marker genes reported as highly specific and stable by at least two recent expression studies 59,60,92,93 and by ImmGen database (www.immgen.org; Figure S9).…”

Microfluidic Single‐Cell Omics Analysis

“…Although the pumpless and structure‐simple microwell facilitates the rapid cell loading, the cell occupancy rate is limited. To overcome this limitation, surface modification, size optimization, dielectrophoretic and acoustic technologies were adopted . For example, Revzin et al utilized poly(ethylene glycol) (PEG) as a molecular linker to modify anti‐CD5 on glass surface for specific T‐cell capture.…”

Combinatorial prediction of marker panels from single‐cell transcriptomic data

“…Shown are the rankings of established marker genes for immune populations generated by different methods used for single-gene marker identification. Data are taken from the spleen tissue of the MCA (Han et al, 2018).…”