Mapping the major proteome of reproductive fluids and sperm membranes of rams: From the cauda epididymis to ejaculation

Tilburg, Maurício Fraga van; Sousa, Solange Damasceno; Lobo, Marina Duarte Pinto; Monteiro-Azevedo, Ana Cristina O.M.; Azevedo, Renato Almeida de; Araújo, Aírton Alencar de; Moura, Arlindo A.

doi:10.1016/j.theriogenology.2020.10.003

Cited by 13 publications

(10 citation statements)

References 58 publications

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“…In the study, proteome profiling of the canine EF was performed using NanoUPLC-Q-TOF/MS for the first time. There are no such reports in world literature, only EF mass spectrometry research in humans [19], boars [20], bulls [21][22][23][24], rams [25,26], and monkeys [27].…”

Section: Discussionmentioning

confidence: 99%

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

Cichowska,

Wisniewski,

Bromke

et al. 2023

IJMS

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Sperm maturation in the epididymis is based on interactions with proteins from epididymal fluid (EF). The aim of the study was to profile canine EF proteome and investigate correlations between EF protein content and epididymal spermatozoa (ES) motion parameters. Twenty-three male dogs were divided into two groups: good sperm motility (GSM) and poor sperm motility (PSM). The total motility and progressive motility differed significantly (p = 0.031; p < 0.001, respectively) between the GSM group and the PSM group. The semen samples were centrifuged to separate the EF apart from the ES. The canine EF proteins were analyzed using nano-liquid chromatography, which was coupled with quadrupole time-of-flight mass spectrometry (NanoUPLC-Q-TOF/MS) and bioinformatic tools for the first time. A total of 915 proteins were identified (GSM—506; PSM—409, respectively). UniProt identification resulted in six unique proteins (UPs) in the GSM group of dogs and four UPs in the PSM group. A semi-quantitative analysis showed a higher abundance (p < 0.05) of four differentially expressed proteins in the GSM group (ALB, CRISP2, LCNL1, PTGDS). Motility-dependent variations were detected in the EF proteome and were related to important metabolic pathways, which might suggest that several proteins could be potential ES motility biomarkers.

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Section: Discussionmentioning

confidence: 99%

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

Cichowska,

Wisniewski,

Bromke

et al. 2023

IJMS

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“…While some authors remark the importance of the seminal plasma in the preservation of motility and viability in ram thawed sperm (Graham, 1994;Maxwell and Watson, 1996;van Tilburg et al, 2021) and the importance of seminal plasma heparin-affinity proteins for the success of fertilization (Martins et al, 2013), other researchers describe harmful effects (Schmehl et al, 1986). Detrimental effects of seminal plasma on motility of liquid stored (Lamirande et al, 1984;Dott et al, 1979;Iwamoto et al, 1993) and frozen-thawed (Roostaei-Ali Mehr and Sharafi, 2013) sperm and viability of liquid stored (Dott et al, 1979) and frozenthawed (Garcia and Graham, 1987;Roostaei-Ali Mehr and Sharafi, 2013) sperm have been reported.…”

Section: Discussionmentioning

confidence: 99%

Reduction of seminal plasma concentration can decrease detrimental effects of seminal plasma on chilled ram spermatozoa

2021

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This study was conducted to investigate the effect of different levels of seminal plasma (SP) and cold-shock on ram spermatozoa during 36 h storage at 5°C. In both ejaculated spermatozoa coated with egg yolk (second ejaculate; coated spermatozoa) and epididymal spermatozoa, samples were treated with 0, 50 and 100% seminal plasma. Different levels of seminal plasma were added on the basis of ram spermatocrit (32%). Then half of aliquots were suddenly put on ice water (cold-shock) and other half were gradually (0.25°C/min) chilled (non-cold shock). Sperm motility, viability and functional membrane integrity were determined in both aliquots at 0, 12, 24 and 36 h storage at 5°C. Under non-cold shock and cold-shock conditions, coated spermatozoa treated with 0% SP showed the highest motility compared to ejaculated spermatozoa (first ejaculate; uncoated spermatozoa) after 12, 24 and 36 h of storage at 5°C (P<0.05). Under non-cold shock and cold-shock conditions, viability and functional membrane integrity was higher in the coated spermatozoa treated with 0% SP than in the uncoated spermatozoa during 36 h storage (P<0.05). There was no significant difference between coated spermatozoa treated with 0 and 50% SP in the percentage of motility and viability after 24 and 36 h of storage (P>0.05). Under non-cold shock and cold-shock conditions, the percentage of motility of epididymal spermatozoa treated with 0% SP was significantly (P<0.05) higher than those treated with 100% SP after 36 h of storage at 5°C. In conclusion, removal of seminal plasma and/or reduction (up to 50%) of its concentration can decrease detrimental effects of seminal plasma on chilled ram spermatozoa.

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“…Sperm acquire proteins from the milieu where they are maintained in the epididymis. Thus, the protein composition of spermatozoa comprises intracellular proteins, membrane proteins of the cell, and proteins attached to them coming from the epididymal and accessory sex gland fluid (Kenny and Byrne, 2018;Rego et al, 2016;van Tilburg et al, 2021). The major trajectory and functional events of sperm cells in a mammalian model are summarized in Figure 1.…”

Section: Sperm Proteome and Fertilitymentioning

confidence: 99%

Identification of biomarkers for bull fertility using functional genomics

et al. 2022

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Prediction of bull fertility is critical for the sustainability of both dairy and beef cattle production. Even though bulls produce ample amounts of sperm with normal parameters, some bulls may still suffer from subpar fertility. This causes major economic losses in the cattle industry because using artificial insemination, semen from one single bull can be used to inseminate hundreds of thousands of cows. Although there are several traditional methods to estimate bull fertility, such methods are not sufficient to explain and accurately predict the subfertility of individual bulls. Since fertility is a complex trait influenced by a number of factors including genetics, epigenetics, and environment, there is an urgent need for a comprehensive methodological approach to clarify uncertainty in male subfertility. The present review focuses on molecular and functional signatures of bull sperm associated with fertility. Potential roles of functional genomics (proteome, small noncoding RNAs, lipidome, metabolome) on determining male fertility and its potential as a fertility biomarker are discussed. This review provides a better understanding of the molecular signatures of viable and fertile sperm cells and their potential to be used as fertility biomarkers. This information will help uncover the underlying reasons for idiopathic subfertility.

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Mapping the major proteome of reproductive fluids and sperm membranes of rams: From the cauda epididymis to ejaculation

Cited by 13 publications

References 58 publications

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

Reduction of seminal plasma concentration can decrease detrimental effects of seminal plasma on chilled ram spermatozoa

Identification of biomarkers for bull fertility using functional genomics

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