Mapping the calcification of bovine pericardium in rat model by enhanced micro-computed tomography

Liu, Jing; Zhong, Shengping; Lan, Hualin; Xu, Meng; Zhang, Haibo; Fan, Yubo; Wang, Yuxing; Wang, Chun-Ren; Wang, Zhaoxu

doi:10.1016/j.biomaterials.2014.06.026

Cited by 12 publications

(16 citation statements)

References 17 publications

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“…We did not observe any cytotoxic effect of GA crosslinked ureteral scaffolds on host fibroblasts or macrophages in vivo . In addition, further disadvantages of GA (e.g., scaffold calcification and depolymerization of GA crosslinks) have been reported (Khor, 1997 ; Schoen and Levy, 2005 ; Liu et al, 2014 ). The data suggest that GA was not an optimal crosslinker for constructive remodeling in the present study (e.g., giant cell and CD68 + macrophage infiltration in vivo , poor biocompatibility in vitro ), which corresponds with other disadvantages described in the literature (Nishi et al, 1995 ; Huang et al, 1998 ; Koch et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Tissue Engineering of Ureteral Grafts: Preparation of Biocompatible Crosslinked Ureteral Scaffolds of Porcine Origin

Koch

Hammer

Oßmann

et al. 2015

Front. Bioeng. Biotechnol.

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The surgical reconstruction of ureteric defects is often associated with post-operative complications and requires additional medical care. Decellularized ureters originating from porcine donors could represent an alternative therapy. Our aim was to investigate the possibility of manufacturing decellularized ureters, the characteristics of the extracellular matrix (ECM) and the biocompatibility of these grafts in vitro/in vivo after treatment with different crosslinking agents. To achieve these goals, native ureters were obtained from pigs and were decellularized. The success of decellularization and the ECM composition were characterized by (immuno)histological staining methods and a DNA-assay. In vitro: scaffolds were crosslinked either with carbodiimide (CDI), genipin (GP), glutaraldehyde, left chemically untreated or were lyophilized. Scaffolds in each group were reseeded with Caco2, LS48, 3T3 cells, or native rat smooth muscle cells (SMC). After 2 weeks, the number of ingrown cells was quantified. In vivo: crosslinked scaffolds were implanted subcutaneously into rats and the type of infiltrating cells were determined after 1, 9, and 30 days. After decellularization, scaffold morphology and composition of ECM were maintained, all cellular components were removed, DNA destroyed and strongly reduced. In vitro: GP and CDI scaffolds revealed a higher number of ingrown 3T3 and SMC cells as compared to untreated scaffolds. In vivo: at day 30, implants were predominantly infiltrated by fibroblasts and M2 anti-inflammatory macrophages. A maximum of MMP3 was observed in the CDI group at day 30. TIMP1 was below the detection limit. In this study, we demonstrated the potential of decellularization to create biocompatible porcine ureteric grafts, whereas a CDI-crosslink may facilitate the remodeling process. The use of decellularized ureteric grafts may represent a novel therapeutic method in reconstruction of ureteric defects.

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Section: Discussionmentioning

confidence: 99%

Tissue Engineering of Ureteral Grafts: Preparation of Biocompatible Crosslinked Ureteral Scaffolds of Porcine Origin

Koch

Hammer

Oßmann

et al. 2015

Front. Bioeng. Biotechnol.

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“…Calcification of glutaraldehyde-fixed tissue implants has been one of their most significant issues since their introduction in the 1960s because of subsequent implant calcification (Schoen et al, 1986;Liu et al, 2014). While the exact mechanism of aldehyde-related calcification is not known, it is thought to be due to a high degree of crosslinking, unreacted aldehyde groups and their attraction of calcium ions, disruption of cellular calcium regulation, localization of calcium in areas of stress and presence of lipids and cell debris (Schmidt and Baier, 2000).…”

Section: Complicationsmentioning

confidence: 99%

“…While the exact mechanism of aldehyde-related calcification is not known, it is thought to be due to a high degree of crosslinking, unreacted aldehyde groups and their attraction of calcium ions, disruption of cellular calcium regulation, localization of calcium in areas of stress and presence of lipids and cell debris (Schmidt and Baier, 2000). Attempts at reducing calcification seem to focus on eliminating cell remnants and lipids and have demonstrated varied success (Arbustini et al, 1984;Chen et al, 1994;Gott et al, 1997;Liu et al, 2014). Though not used in EIR, the use of glutaraldehyde-fixed products in bioprosthetic valves eventually results in calcification (Golomb et al, 1987;Schoen et al, 1992;Girardot et al, 1995;Bonetti et al, 2019).…”

Section: Complicationsmentioning

confidence: 99%

Promoting Cardiac Regeneration and Repair Using Acellular Biomaterials

Vasanthan

Hassanabad

Pattar

et al. 2020

Front. Bioeng. Biotechnol.

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Ischemic heart disease is a common cause of end-stage heart failure and has persisted as one of the main causes of end stage heart failure requiring transplantation. Maladaptive myocardial remodeling due to ischemic injury involves multiple cell types and physiologic mechanisms. Pathogenic post-infarct remodeling involves collagen deposition, chamber dilatation and ventricular dysfunction. There have been significant improvements in medication and revascularization strategies. However, despite medical optimization and opportunities to restore blood flow, physicians lack therapies that directly access and manipulate the heart to promote healthy post-infarct myocardial remodeling. Strategies are now arising that use bioactive materials to promote cardiac regeneration by promoting angiogenesis and inhibiting cardiac fibrosis; and many of these strategies leverage the unique advantage of cardiac surgery to directly visualize and manipulate the heart. Although cellular-based strategies are emerging, multiple barriers exist for clinical translation. Acellular materials have also demonstrated preclinical therapeutic potential to promote angiogenesis and attenuate fibrosis and may be able to surmount these translational barriers. Within this review we outline various acellular biomaterials and we define epicardial infarct repair and intramyocardial injection, which focus on administering bioactive materials to the cardiac epicardium and myocardium respectively to promote cardiac regeneration. In conjunction with optimized medical therapy and revascularization, these techniques show promise to upregulate pathways of cardiac regeneration to preserve heart function.

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“…A limitation of this study is the lack of information about long-term durability of scCO 2 treated pericardium including tissue degradation and calcification of pericardia. A mineralisation assay can provide some information on this calcification process, together with culturing cells in (salt enriched) simulated body fluid solution to enhance calcification process [47], as well as X-ray spectroscopy [48]. However, laboratory tests are only limited in predictability of calcification and in vivo studies should address induction of scar tissue, induction of a pro-inflammatory reaction or hypothesised full remodelling aspects of scCO 2 decellularised pericardium.…”

Section: Long Term Durabilitymentioning

confidence: 99%

Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

Halfwerk

Rouwkema

Gossen³

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Mapping the calcification of bovine pericardium in rat model by enhanced micro-computed tomography

Cited by 12 publications

References 17 publications

Tissue Engineering of Ureteral Grafts: Preparation of Biocompatible Crosslinked Ureteral Scaffolds of Porcine Origin

Tissue Engineering of Ureteral Grafts: Preparation of Biocompatible Crosslinked Ureteral Scaffolds of Porcine Origin

Promoting Cardiac Regeneration and Repair Using Acellular Biomaterials

Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

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