Mapping RNA-Protein Interactions with Subcellular Resolution Using Colocalization CLIP
Soon Yi,
Shashi S. Singh,
Kathryn Rozen-Gagnon
et al.
Abstract:RNA binding proteins (RBPs) are essential for RNA metabolism and profoundly impact health and disease. The subcellular organization of RBP interaction networks with target RNAs remains largely unexplored. Here, we develop colocalization CLIP, a method that combines CrossLinking and ImmunoPrecipitation (CLIP) with proximity labeling, to explore in-depth the subcellular RNA interactions of the well-studied RNA-binding protein HuR. Using this method, we uncover HuR's dynamic and location-specific interactions wit… Show more
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