Mapping QTLs for resistance against Globodera pallida (Stone) Pa2/3 in a diploid potato progeny originating from Solanum spegazzinii

Abstract

A "F1" diploid population between Solanum tuberosum 2 x and the wild Solanum spegazzinii was studied. It segregated for resistance against the potato cyst nematode Globodera pallida derived from the wild species. The inheritance had a quantitative nature. Linkage maps of AFLP and RFLP markers were constructed for both parents. Three QTLs were identified on the map of the resistant parent on chromosomes V, VI and XII, respectively. The first one had a major effect and explained more than 50% of the total variance of resistance. It is located in a cluster of resistance genes and may be the same locus as Gpa which has been described formerly. The two others explained about 20% of the total variance each. The QTL on chromosome XII is also in a cluster of resistance genes, and in an orthologous position with resistance genes against nematodes in tomato and pepper.

Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3

“…One or more members of the R1 gene family are good positional candidates for being the molecular basis also of the genes for nematode resistance located in this region, similarly as has been found for genes Rx1 for resistance to Potato Virus X (PVX) and Gpa2 for resistance to G. pallida pathotype Pa2, which are both highly homologous members of a clustered NB-LRR type gene family on potato chromosome XII . To the same genomic region on potato chromosome V as identified in this paper, major QTL for resistance to G. pallida and G. rostochiensis have been mapped previously, which originated from the wild species S. spegazzinii, S. vernei, S. sparsipilum and possibly others (Kreike et al 1994;Rouppe van der Voort et al 1998Bryan et al 2002;Caromel et al 2003;. S. vernei is the most likely source of the resistance allele present in breeding clone SR6 and in most varieties with high resistance to G. pallida pathotype Pa2/3.…”

“…A number of QTL (quantitative trait locus) mapping experiments for resistance to G. pallida have been performed in different, mostly diploid genetic backgrounds (Kreike et al 1994;Bradshaw et al 1998;Rouppe van der Voort et al 1998Bryan et al 2002Bryan et al , 2004Caromel et al 2003Caromel et al , 2005. The major gene Gpa2 for resistance to G. pallida pathotype Pa2 on chromosome XII has been cloned and shown to be a member of the NB (nucleotide binding) -LRR (leucine-rich-repeat) gene family, to which most known plant genes for pathogen resistance belong .…”

Integration of Two Diploid Potato Linkage Maps with the Potato Genome Sequence

“…Most potato linkage maps have been generated from diploid populations to simplify genetic segregation and to incorporate polymorphism from wild species and primitive cultivars. These maps range in size from 606 cM [2] to 1120 cM [4] and contain as few as 85 markers [6] and as many as 10,000 markers [5]. Potato linkage maps have been constructed from many types of markers, including isozymes, Restriction Fragment Length Polymorphisms (RFLPs), Simple Sequence Repeats (SSRs), Amplified Fragment Length Polymorphisms (AFLPs) and more recently, Single Nucleotide Polymorphisms (SNPs) [2], [7], [5], [8].…”

Using SNP markers to dissect linkage disequilibrium at a major quantitative trait locus for resistance to the potato cyst nematode Globodera pallida on potato chromosome V

“…Molecular mapping has repeatedly revealed a major quantitative resistance locus (QRL) against G. pallida on potato chromosome V (Kreike et al 1994;Rouppe van der Voort et al 1998, 2000Caromel et al 2003;Sattarzadeh et al 2006). This QRL is tagged by the DNA markers GP21 and GP179 Xanking a genetic interval of 3 cM (Meksem et al 1995).…”