Mapping of the New Fertility Restorer Gene Rf-PET2 Close to Rf1 on Linkage Group 13 in Sunflower (Helianthus annuus L.)

Sajer, Osama; Schirmak, Uta; Hamrit, Sonia; Horn, Renate

doi:10.3390/genes11030269

Cited by 5 publications

(4 citation statements)

References 66 publications

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“…Most of the 98 RFL genes identified in the sunflower (Helianthus annuus) genome are located within three big clusters on chromosomes 3, 7 and 13, and have RfCTDs (Supplementary Table 3). The genomic locations of the RFL clusters coincide with the mapped positions of multiple restorer genes reported to be involved in fertility restoration of the PET1 and PET2 CMS types in the species (Sajer et al 2020). This includes Rf1, Rf-PET2 and Rf5 mapped on chromosome 13, Rf3 and Msc1 on chromosome 7 and Rf4 on chromosome 3, respectively (Sajer et al 2020).…”

Section: Correlation Of Numbers Of Rfls/rfctds With Reproductive Stra...mentioning

confidence: 54%

“…The genomic locations of the RFL clusters coincide with the mapped positions of multiple restorer genes reported to be involved in fertility restoration of the PET1 and PET2 CMS types in the species (Sajer et al 2020). This includes Rf1, Rf-PET2 and Rf5 mapped on chromosome 13, Rf3 and Msc1 on chromosome 7 and Rf4 on chromosome 3, respectively (Sajer et al 2020). Similar to carrot, the genomic regions carrying restorer genes in cereals, such as wheat, barley, and rye, overlap with the locations of RFL clusters (Melonek and Small 2022).…”

Section: Correlation Of Numbers Of Rfls/rfctds With Reproductive Stra...mentioning

confidence: 54%

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A unique C-terminal domain contributes to the molecular function of restorer-of-fertility proteins in plant mitochondria

Huynh

Melonek

Francs‐Small

et al. 2023

Preprint

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Restorer-of-fertility (Rf) genes have practical applications in hybrid seed production as a means to control self-pollination. They encode pentatricopeptide repeat (PPR) proteins that are targeted to mitochondria where they specifically bind to transcripts that induce cytoplasmic male sterility and repress their expression. In searching for a molecular signature unique to this class of proteins, we found that a majority of known Rf proteins have a unique domain, which we called RfCTD (Restorer-of-fertility C-terminal domain), and its presence correlates with the ability to induce cleavage of the mitochondrial RNA target. We constructed a sequence profile that can quickly and accurately identify RfCTD sequences in plant genomes or transcriptomes. We screened 219 angiosperm genomes from 123 genera and found that each diploid genome encodes, on average, 25 Rf-like (RFL) proteins, of which approximately 55% contain the C-terminal signature domain. This screen also revealed considerable variation in RFL gene numbers across flowering plants. We observed that plant genera with bisexual flowers have significantly higher numbers of RFL genes compared to those with unisexual flowers, consistent with a role of these proteins in restoration of male fertility. Finally, we show that removing the RfCTD from the RFL protein RNA PROCESSING FACTOR 2-nad6 prevented cleavage of its RNA target, the nad6 transcript, in Arabidopsis thaliana mitochondria. This research provides a simple way of identifying putative Rf candidates in genome sequences, new insights into the molecular mode of action of Rf proteins in plant mitochondria and expands our understanding of the evolution of fertility restoration in flowering plants.

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Section: Correlation Of Numbers Of Rfls/rfctds With Reproductive Stra...mentioning

confidence: 54%

Section: Correlation Of Numbers Of Rfls/rfctds With Reproductive Stra...mentioning

confidence: 54%

A unique C-terminal domain contributes to the molecular function of restorer-of-fertility proteins in plant mitochondria

Huynh

Melonek

Francs‐Small

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Most of the RFL genes identified in the sunflower ( Helianthus annuus ) genome are located within three big clusters on chromosomes 3, 7 and 13, and have RfCTDs (Table S5). The genomic locations of the RFL clusters coincide with the mapped positions of multiple restorer genes reported to be involved in fertility restoration of the PET1 and PET2 CMS types in the species (Sajer et al ., 2020). Similar to carrot, the genomic regions carrying restorer genes in cereals overlap with the locations of RFL clusters (Melonek & Small, 2022).…”

Section: Discussionmentioning

confidence: 99%

A unique C‐terminal domain contributes to the molecular function of Restorer‐of‐fertility proteins in plant mitochondria

et al. 2023

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Summary Restorer‐of‐fertility (Rf) genes encode pentatricopeptide repeat (PPR) proteins that are targeted to mitochondria where they specifically bind to transcripts that induce cytoplasmic male sterility and repress their expression. In searching for a molecular signature unique to this class of proteins, we found that a majority of known Rf proteins have a distinct domain, which we called RfCTD (Restorer‐of‐fertility C‐terminal domain), and its presence correlates with the ability to induce cleavage of the mitochondrial RNA target. A screen of 219 angiosperm genomes from 123 genera using a sequence profile that can quickly and accurately identify RfCTD sequences revealed considerable variation in RFL/RfCTD gene numbers across flowering plants. We observed that plant genera with bisexual flowers have significantly higher numbers of RFL genes compared to those with unisexual flowers, consistent with a role of these proteins in restoration of male fertility. We show that removing the RfCTD from the RFL protein RNA PROCESSING FACTOR 2‐nad6 prevented cleavage of its RNA target, the nad6 transcript, in Arabidopsis thaliana mitochondria. We provide a simple way of identifying putative Rf candidates in genome sequences, new insights into the molecular mode of action of Rf proteins and the evolution of fertility restoration in flowering plants.

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“…The nature of all tested genotypes was confirmed by all four KASP assays, with the exception of restorer line IH-51, which grouped with the maintainer line. This line is a restorer line for both PET1 and PET2 cytoplasm [ 31 ], and obviously does not possess the Rf1 gene, but the Rf-PET2 gene that is positioned close to Rf1 , mapping on the lower part of LG 13 [ 34 ]. Consequently, fertility restoration of PET1 in IH-51 must occur via a different restorer gene.…”

Section: Discussionmentioning

confidence: 99%

KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)

Radanović

Sprycha

Jocković

et al. 2022

Genes

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Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.

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Mapping of the New Fertility Restorer Gene Rf-PET2 Close to Rf1 on Linkage Group 13 in Sunflower (Helianthus annuus L.)

Cited by 5 publications

References 66 publications

A unique C-terminal domain contributes to the molecular function of restorer-of-fertility proteins in plant mitochondria

A unique C-terminal domain contributes to the molecular function of restorer-of-fertility proteins in plant mitochondria

A unique C‐terminal domain contributes to the molecular function of Restorer‐of‐fertility proteins in plant mitochondria

KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)

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