Mapping of the Na+, K+-ATPase subunit α 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis

Davoli, Roberta; Zijlstra, C.; Bosma, Anneke A.; Russo, Vincenzo

doi:10.1046/j.1365-2052.1999.00407.x

Cited by 9 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PCR results obtained for the three genes using the porcine-rodent somatic cell hybrid panel were analyzed using the IMpRH mapping tool ( http://imprh.toulouse.inra.fr/ ). This suggested a chromosomal localization of ATP1A1 and ATP1A2 to porcine chromosome 4, which is in agreement with previous studies reporting ATP1A1 to be localized to porcine 4q16-q23 and ATP1A2 to be localized to porcine 4q21-q23 [39] , [40] . We found ATP1A1 linked to SW2435 (LOD score 4.01, distance 0.72 ray) and ATP1A2 linked to SW589 (LOD score 11.64, distance 0.26 ray).…”

Section: Resultssupporting

confidence: 92%

Molecular Cloning and Characterization of Porcine Na+/K+-ATPase Isoforms α1, α2, α3 and the ATP1A3 Promoter

et al. 2013

View full text Add to dashboard Cite

Na+/K+-ATPase maintains electrochemical gradients of Na+ and K+ essential for a variety of cellular functions including neuronal activity. The α-subunit of the Na+/K+-ATPase exists in four different isoforms (α1–α4) encoded by different genes. With a view to future use of pig as an animal model in studies of human diseases caused by Na+/K+-ATPase mutations, we have determined the porcine coding sequences of the α1–α3 genes, ATP1A1, ATP1A2, and ATP1A3, their chromosomal localization, and expression patterns. Our ATP1A1 sequence accords with the sequences from several species at five positions where the amino acid residue of the previously published porcine ATP1A1 sequence differs. These corrections include replacement of glutamine 841 with arginine. Analysis of the functional consequences of substitution of the arginine revealed its importance for Na+ binding, which can be explained by interaction of the arginine with the C-terminus, stabilizing one of the Na+ sites. Quantitative real-time PCR expression analyses of porcine ATP1A1, ATP1A2, and ATP1A3 mRNA showed that all three transcripts are expressed in the embryonic brain as early as 60 days of gestation. Expression of α3 is confined to neuronal tissue. Generally, the expression patterns of ATP1A1, ATP1A2, and ATP1A3 transcripts were found similar to their human counterparts, except for lack of α3 expression in porcine heart. These expression patterns were confirmed at the protein level. We also report the sequence of the porcine ATP1A3 promoter, which was found to be closely homologous to its human counterpart. The function and specificity of the porcine ATP1A3 promoter was analyzed in transgenic zebrafish, demonstrating that it is active and drives expression in embryonic brain and spinal cord. The results of the present study provide a sound basis for employing the ATP1A3 promoter in attempts to generate transgenic porcine models of neurological diseases caused by ATP1A3 mutations.

show abstract

Section: Resultssupporting

confidence: 92%

Molecular Cloning and Characterization of Porcine Na+/K+-ATPase Isoforms α1, α2, α3 and the ATP1A3 Promoter

et al. 2013

View full text Add to dashboard Cite

show abstract

“…1998a). The localization of ATP1A2 to Sscr 4q21–q23 is a refinement of the assignment already obtained on the Sscr 4 (Fontanesi et al . 1999).…”

Section: Resultssupporting

confidence: 71%

“…1999; FHL1 on Sscr X, Krempler et al . 2000) or by our group using a different somatic cell hybrid panel ( PFKM on Sscr 5, Fontanesi et al . 1999) or by in situ hybridization ( PSMA4 on Sscr 7, Davoli et al .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig

et al. 2002

Self Cite

View full text Add to dashboard Cite

To identify genes with effects on meat quality and production traits we developed an adult porcine skeletal muscle cDNA library. After pre-screening this library with seven genes highly expressed in skeletal muscle, 385 non-hybridizing clones were sequenced from both ends to yield 510 expressed sequence tags (ESTs). Together with those ESTs previously generated from this library, we have produced 701 porcine skeletal muscle ESTs. These ESTs were grouped into 306 different cDNA species and compared with the human skeletal muscle transcriptional profiles obtained from different databases. Furthermore we mapped 107 of these cDNAs using a somatic cell hybrid panel with genes mapping over all the autosomes (except on chromosome 11) and on chromosome X. The mapping of these cDNAs contributed to the construction of a first genomic transcript map of the skeletal muscle tissue in pig.

show abstract

“…ATP1A1 , ATP1A2 and ATP1B1 have been mapped to SSC4 (Lahbib‐Mansais et al. 1993; Fontanesi et al. 1999).…”

Section: Resultsmentioning

confidence: 99%

Mapping of quantitative trait loci for clinical–chemical traits in swine

et al. 2009

View full text Add to dashboard Cite

Clinical-chemical traits are diagnostic parameters essential for characterization of health and disease in veterinary practice. The traits show significant variability and are under genetic control, but little is known about the fundamental genetic architecture of this variability, especially in swine. We have identified QTL for alkaline phosphatase (ALP), lactate (LAC), bilirubin (BIL), creatinine (CRE) and ionized sodium (Na(+)), potassium (K(+)) and calcium (Ca(++)) from the serum of 139 F(2) pigs from a Meishan/Pietrain family before and after challenge with Sarcocystis miescheriana, a protozoan parasite of muscle. After infection, the pigs passed through three stages representing acute disease, subclinical disease and chronic disease. Forty-two QTL influencing clinical-chemical traits during these different stages were identified on 15 chromosomes. Eleven of the QTL were significant on a genome-wide level; 31 QTL were chromosome-wide significant. QTL showed specific health/disease patterns with respect to the baseline values of the traits as well as the values obtained through the different stages of disease. QTL influencing different traits at different times were found primarily on chromosomes 1, 3, 7 and 14. The most prominent QTL for the investigated clinical-chemical traits mapped to SSC3 and 7. Baseline traits of ALP, LAC, BIL, Ca(++) and K(+) were influenced by QTL regions on SSC3, 6, 7, 8 and 13. Single QTL explained up to 21.7% of F(2) phenotypic variance. Our analysis confirms that variation of clinical-chemical traits is associated with multiple chromosomal regions.

show abstract

Mapping of the Na⁺, K⁺-ATPase subunit α 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis

Cited by 9 publications

References 14 publications

Molecular Cloning and Characterization of Porcine Na+/K+-ATPase Isoforms α1, α2, α3 and the ATP1A3 Promoter

Molecular Cloning and Characterization of Porcine Na+/K+-ATPase Isoforms α1, α2, α3 and the ATP1A3 Promoter

Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig

Mapping of quantitative trait loci for clinical–chemical traits in swine

Contact Info

Product

Resources

About