Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes

Abstract

The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines. The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F 2 population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used to identify DNA markers linked to the ms8 locus. With the use of RAPD-BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however, at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for ms8 gene cloning.

Mapping of male sterility gene ms10 in chilli pepper (Capsicum annuum L.)

“…(,b,c) using amplified fragment length polymorphism (AFLP) analysis reported 2–4% polymorphism between MF and MS bulks; Bartoszewski et al. () using random amplified polymorphic DNA (RAPD) analysis reported 5.6% parental polymorphism.…”

“…Bartoszewski et al. () identified seven markers linked to the ms8 locus. Comparative mapping using reference pepper maps of Lefebvre et al.…”

“…Of the tagged MS genes, only ms8 has been mapped on chromosome 4 (Bartoszewski et al. ). Molecular markers linked to the ms10 gene and its map position in the Capsicum are still not known.…”

“…For this reason, only a few NMS lines have been developed in chilli and utilized for hybrid seed production (Dhaliwal and Jindal 2014 (Bartoszewski et al 2012) and an undesignated gene (Lee et al 2010c), have been identified. Of the tagged MS genes, only ms8 has been mapped on chromosome 4 (Bartoszewski et al 2012). Molecular markers linked to the ms10 gene and its map position in the Capsicum are still not known.…”

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

“…Molecular markers have been developed and will help the introgression of ms genes in different backgrounds and selection of sterile plants in hybrid seed production (Bartoszewski et al. , Aulakh et al. ).…”