Mapping of the humanGSPT1 gene, a human homolog of the yeastGST1 gene, to chromosomal band 16p13.1

Ozawa, Kazuo; Murakami, Yasufumi; Eki, Toshihiko; Yokoyama, Kazushige; Soeda, Eiichi; Hoshino, Shin‐ichi; Ui, Michio; Hanaoka, Fumio

doi:10.1007/bf01233164

Cited by 14 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings allowed us to postulate that the previously identified human GSPT1 may also function as eRF3. However, our further studies revealed the existence of another GSPT gene in mammalian cells (14). In the present paper, we have isolated two mouse GSPT genes, the counterpart of human GSPT1 and a novel member of the GSPT gene family, GSPT2.…”

mentioning

confidence: 74%

“…In contrast to GSPT1, the expression of the novel GSPT2 gene was constant during the cell-cycle progression of 3T3 cells and was relatively abundant in mouse brain. Our previous study on the chromosome mapping of the GSPT1 gene suggested the existence of a homologous gene on the X chromosome (14), which may represent the locus of human GSPT2 gene.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Molecular Cloning of a Novel Member of the Eukaryotic Polypeptide Chain-Releasing Factors (eRF)

Hoshino¹,

Imai²,

Mizutani³

et al. 1998

Journal of Biological Chemistry

Self Cite

101

131

View full text Add to dashboard Cite

Yeast GST1 gene, whose product is a GTP-binding protein structurally related to polypeptide chain elongation factor-1␣ (EF1␣), was first described to be essential for the G 1 to S phase transition (GSPT) of the cell cycle, and the product was recently reported to function as a polypeptide chain release factor 3 (eRF3) in yeast. Although we previously cloned a human homologue (renamed as GSPT1) of the yeast gene, it has remained to be determined whether GSPT1 also functions as eRF3 or if another GSPT may have such a function in mammalian cells. In the present study, we isolated two mouse GSPT genes, the counterpart of human GSPT1 and a novel member of the GSPT gene family, GSPT2. Both the mouse GSPTs had a two-domain structure characterized as an amino-terminal no-homologous region (approximately 200 amino acids) and a carboxyl-terminal conserved eukaryotic elongation factor-1␣-like domain (428 amino acids). Messenger RNAs of the two GSPTs could be detected in all mouse tissues surveyed, although the level of GSPT2 message appeared to be relatively abundant in the brain. The mouse GSPT1 was expressed in a proliferation-dependent manner in Swiss 3T3 cells, whereas the expression of GSPT2 was constant during the cell-cycle progression. Immunoprecipitation assays in COS-7 cells expressing flag epitope-tagged proteins demonstrated that not only GSPT1 but also GSPT2 was capable of interacting with eRF1. Such interaction between GSPT2 and eRF1 was also confirmed by yeast two-hybrid analysis. Taken together, these data indicated that the novel GSPT2 may interact with eRF1 to function as eRF3 in mammalian cells.

show abstract

mentioning

confidence: 74%

Section: Discussionmentioning

confidence: 98%

Molecular Cloning of a Novel Member of the Eukaryotic Polypeptide Chain-Releasing Factors (eRF)

Hoshino¹,

Imai²,

Mizutani³

et al. 1998

Journal of Biological Chemistry

Self Cite

101

131

View full text Add to dashboard Cite

show abstract

“…1. The proximal limit of the commonly deleted region was defined by D16S519, on the basis of observations in six tumors (3,5,15,18,32, and 77) that retained heterozygosity at the D16S519 locus while showing LOH at more distal loci. The distal limit was defined by D16S3078; three tumors (29,30, and 100) retained heterozygosity at D16S3078 while showing LOH at more proximal loci.…”

Section: Resultsmentioning

confidence: 99%

“…1,31) and GSPT1 at 16p13. 1,32) although no direct evidence has yet been demonstrated for a role of these genes in hepatocarcinogenesis. In addition, several genes or expressed sequence tags (ESTs) were mapped in the vicinity of the commonly deleted region defined in the present study; these includes pyrroline-5-carboxylate dehydrogenase, calcium-regulated heat-stable protein, N-methyl-D-aspartate receptor modulator, LPS-induced TNF-α factor, and epithelial membrane 2.…”

Section: Discussionmentioning

confidence: 99%

Localization of a Target Region of Allelic Loss to a 1‐cM Interval on Chromosome 16p.13.13 in Hepatocellular Carcinoma

Koyama

Nagai

Bando

et al. 1999

Japanese Journal of Cancer Research

View full text Add to dashboard Cite

To identify the location of the putative tumor suppressor gene on chromosome 16p that may be involved in hepatocellular carcinoma (HCC), we examined 96 primary HCCs and evaluated their patterns of allelic loss at 10 microsatellite marker loci distributed along this chromosome arm. Allelic loss at one or more loci was observed in 46 (48%) of these tumors. Through detailed deletion mapping of tumors having partial or interstitial deletions, we identified a commonly deleted region at a 1-cM interval, flanked by D16S519 and D16S3078 at 16p13.13, defining the location of a putative tumor suppressor gene for HCC. This region contains the gene for JAB (JAK-binding protein), which is responsible for negative-feedback regulation of the JAK-STAT pathway induced by cytokine stimulation, raising the possibility that inactivation of this gene may participate in hepatocarcinogenesis via genetic and/or epigenetic changes.

show abstract

“…In humans, eRF3 has two distinct isoforms, eRF3a encoded by eRF3a/GSPT1 gene located in 16p13.1 (11) and eRF3b, encoded by eRF3b/GSPT2 gene located in Xp11. 21-23 (12).…”

Section: Introductionmentioning

confidence: 99%

eRF3a/GSPT1 12-GGC allele increases the susceptibility for breast cancer development

Brito¹

2009

Oncol Rep

View full text Add to dashboard Cite

Abstract.It is now widely recognized that translation factors are involved in cancer development and that components of the translation machinery that are deregulated in cancer cells may become targets for cancer therapy. The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. eRF3a/ GSPT1 first exon contains a (GGC)n expansion coding for proteins with different N-terminal extremities. Herein we show that the longer allele (12-GGC) is present in 5.1% (7/137) of the breast cancer patients analysed and is absent in the control population (0/135), corresponding to an increased risk for cancer development, as revealed by Odds Ratio analysis. mRNA quantification suggests that patients with the 12-GGC allele overexpress eRF3a/GSPT1 in tumor tissues relative to the normal adjacent tissues. However, using an in vivo assay for translation termination in HEK293 cells, we do not detect any difference in the activity of the eRF3a proteins encoded by the various eRF3a/GSPT1 alleles. Although the connection between the presence of eRF3a/GSPT1 12-GGC allele and tumorigenesis is still unknown, our data suggest that the presence of the 12-GGC allele provides a potential novel risk marker for various types of cancer.

show abstract

Mapping of the humanGSPT1 gene, a human homolog of the yeastGST1 gene, to chromosomal band 16p13.1

Cited by 14 publications

References 17 publications

Molecular Cloning of a Novel Member of the Eukaryotic Polypeptide Chain-Releasing Factors (eRF)

Molecular Cloning of a Novel Member of the Eukaryotic Polypeptide Chain-Releasing Factors (eRF)

Localization of a Target Region of Allelic Loss to a 1‐cM Interval on Chromosome 16p.13.13 in Hepatocellular Carcinoma

eRF3a/GSPT1 12-GGC allele increases the susceptibility for breast cancer development

Contact Info

Product

Resources

About