Mapping of the human VLA‐α4 gene to chromosome 2q31‐q32

Fernández‐Ruiz, Elena; Villena, Fernando P Pardo Manuel De; Rubio, Miguel A.; Corbí, Ángel L.; Córdoba, Santiago Rodrı́guez de; Sánchez‐Madrid, Francisco

doi:10.1002/eji.1830220243

Cited by 10 publications

(3 citation statements)

References 24 publications

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“…After heat denaturation, 4 ng/gl of labeled probe was preannealed with a 500-fold excess of sonicated human placental DNA for 1 h at 37~ and hybridized overnight to denatured metaphase chromosomes from a normal male. Hybridization, washings, and detection with rhodamine-conjugated antibodies were performed as described (30). Chromosomes were counterstained with 75 ng/ml of 4'-6-diamino-2-phenylindole (DAPI) in antifade medium.…”

Section: Methodsmentioning

confidence: 99%

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Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors.

López–Cabrera

Santis

Fernández‐Ruiz

et al. 1993

The Journal of Experimental Medicine

268

168

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SuntmaryThe activation of T lymphocytes, both in vivo and in vitro, induces the expression of CD69. This molecule, which appears to be the earliest inducible cell surface glycoprotein acquired during lymphoid activation, is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. To determine the structural basis for CD69 function, the cDNA coding for CD69 was isolated by a polymerase chain reaction-based strategy using oligonucleotides deduced from peptide sequences of the purified protein. The isolated cDNA exhibited a single open reading frame of 597 bp coding for CD69, and predicted a 199-amino acid protein of type II membrane topology, with extracellular (COOH-terminal), transmembrane, and intracellular domains. The CD69 clone hybridized to a 1.7-kb mRNA species, which was rapidly induced and degraded after lymphocyte stimulation, consistent with the presence of rapid degradation signals at the 3' untranslated region. Transient expression of the polypeptide encoded by CD69 cDNA in COS-7 cells demonstrated that it presented properties comparable to native CD69 protein. The CD69 gene was regionally mapped to chromosome 12 p13-p12 by both somatic cell hybrid DNA analysis and fluorescence in situ hybridization coupled with GTG banding (G bands by trypsin using Giemsa). Protein sequence homology search revealed that CD69 is a new member of the Ca2+-dependent (C-type) lectin superfamily of type II transmembrane receptors, which includes the human NKG2, the rat NKR-P1, and the mouse NKR-P1 families of NK cell-specific genes. CD69 also has structural homology with other type II lectin cell surface receptors, such as the T cell antigen Ly49, the low avidity immunoglobulin E receptor (CD23), and the hepatic asialoglycoprotein receptors. The CD69 protein also shares functional characteristics with most members of this superfamily, which act as transmembrane signaling receptors in early phases of cellular activation.

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Section: Methodsmentioning

confidence: 99%

“…Chromosomes were counterstained with 75 ng/ml of 4'-6-diamino-2-phenylindole (DAPI) in antifade medium. After the fluorescent microscopy, GTG banding was performed as described (30).…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors.

López–Cabrera

Santis

Fernández‐Ruiz

et al. 1993

The Journal of Experimental Medicine

268

168

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“…Post-hybridization washes were done three times for 5 min in 50% formamide, 2 x SSC pH 7 at 45 "C, followed by washing €or 5 min in 0.1 x SSC at 60°C and twice for 5 min in 2 X SSC at room temperature. Immunochemical detection with tetramethyl rhodamine isothiocyanate (TR1TC)-labeled anti-digoxigenin antibodies was performed as described [20]. GTG banding was done after hybridization in situ as previously described [21].…”

Section: Hybridization In Situ and Immunocytochemical Detectionmentioning

confidence: 99%

CD28/CTLA‐4 ligands: the gene encoding CD86 (B70/B7.2) maps to the same region as CD80 (B7/B7.1) gene in human chromosome 3q13‐q23

et al. 1995

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CD86 (B70/B7.2) is an antigen of the immunoglobulin superfamily expressed on monocytes, dendritic cells and activated B, T, and natural killer cells. CD86 was recently identified as a second ligand for the T cell antigens CD28 and CTLA-4, and plays an important role in the co-stimulation of T cells in a primary immune response. We report here the assignment of the CD86 gene to human chromosome 3 using Southern blot analysis on a panel of hamster x human somatic cell hybrid genomic DNA. Fluorescence hybridization in situ on metaphase chromosomes coupled with GTG banding (G-bands by trypsin using Giemsa staining) confirmed this assignment and localized the CD86 gene to 3q13-q23 region. The CD86 gene is, therefore, located in the proximity of the CD80 (B7/B7.1) gene, the first identified ligand for CD28 and CTLA-4, previously mapped to chromosome 3q13.3-q21. Deletions, inversions and insertions of chromosome 3q21-q26, as well as translocations of 3q21 with other chromosomes have been described in many cases of acute myeloid leukemia (AML), acute non-lymphocytic leukemia (ANLL), chronic myeloid leukemia (CML) and myelodisplastic syndromes (MDS), suggesting that this region contains several genes involved in the leukemic process.

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Mapping of the human VLA‐α4 gene to chromosome 2q31‐q32

Cited by 10 publications

References 24 publications

Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors.

Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors.

CD28/CTLA‐4 ligands: the gene encoding CD86 (B70/B7.2) maps to the same region as CD80 (B7/B7.1) gene in human chromosome 3q13‐q23

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