Mapping of single cells by near infrared Raman microspectroscopy

Krafft, Christoph; Knetschke, Thomas; Siegner, Axel; Funk, Richard; Salzer, Reiner

doi:10.1016/s0924-2031(03)00049-3

Cited by 171 publications

(163 citation statements)

References 18 publications

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“…The resulting spectra showed the localisation of the nucleus, organelles and the membrane (Kraft et al, 2003). Raman spectroscopy is more often associated with the determination of molecular structure and with qualitative analysis; as an example, the conformational characterisation of natively unfolded proteins through a simple band fitting of the amide I region has been recently described (Maiti et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus

et al. 2006

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Several techniques are under development to diagnose oesophageal adenocarcinoma at an earlier stage. We have demonstrated the potential of Raman spectroscopy, an optical diagnostic technique, for the identification and classification of malignant changes. However, there is no clear recognition of the biochemical changes that distinguish between the different stages of disease. Our aim is to understand these changes through Raman mapping studies. Raman spectral mapping was used to analyse 20-mm sections of tissue from 29 snap-frozen oesophageal biopsies. Contiguous haematoxylin and eosin sections were reviewed by a consultant pathologist. Principal component analysis was used to identify the major differences between the spectra across each map. Pseudocolour score maps were generated and the peaks of corresponding loads identified enabling visualisation of the biochemical changes associated with malignancy. Changes were noted in the distribution of DNA, glycogen, lipids and proteins. The mean spectra obtained from selected regions demonstrate increased levels of glycogen in the squamous area compared with increased DNA levels in the abnormal region. Raman spectroscopy is a highly sensitive and specific technique for demonstration of biochemical changes in the carcinogenesis of Barrett's oesophagus. There is potential for in vivo application for real-time endoscopic optical diagnosis.

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Section: Discussionmentioning

confidence: 99%

Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus

et al. 2006

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“…Details of the instrumentation, the analysis, and data from cells in medium have been published elsewhere (38 ). Similar mapping experiments on fixed single cells have also been described by another group (39).…”

Section: Raman Microspectroscopy Of Single Cellsmentioning

confidence: 82%

Raman Spectroscopy as a New Biochemical Diagnostic Tool

Uskoković‐Marković¹,

Jelikić-Stankov²,

Holclajtner-Antunović³

et al. 2013

Journal of Medical Biochemistry

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SummaryIn this review, Raman spectroscopy is described as a new and potentially powerful diagnostic tool in comparison to routine biochemical tests. Advanced instrumentation and new Raman spectroscopy techniques enable rapid and simultaneous identification and/or determination of se veral biochemical parameters, such as glucose, acetone, creatinine, urea, lipid profile, uric acid, total protein, etc, with a very low limit of detection. Raman spectroscopy could also be applied in molecule and cell characterization, as well as diagnostics of atherosclerosis in its early stage. Raman spectroscopy is nondestructive and could be applied to all kinds of samples, which simplifies the diagnostics of numerous disea ses and pathologic states. Special attention is paid to literature data illustrating the application of Raman spectroscopy for transdermal glucose monitoring and cancer diagnostics.Keywords: biochemical parameters, cancer diagnosis, glu cose monitoring, Raman spectroscopy Kratak sadr`aj U ovom prikazu opisana je primena Ramanske spektroskopije kao nove metode velikih mogu}nosti u dijagnostici, u pore|enju sa rutinskim biohe mijskim testovima. Me toda je razvijena i usavr{ena za identifikaciju i/ili odre |ivanje velikog broja biohemijskih parametara, kao {to su glu koza, aceton, kreatinin, urea, lipidni profil, mokra}na kiselina, ukupni proteini i drugi, uz veoma nizak limit detekcije. Ramanska spektroskopija tako|e se mo`e primenjivati u molekulskoj i }elij -skoj karakterizaciji, kao i za dijagnostiku ranog stadijuma ateroskleroze. Ramanska spektroskopija je nedestruktivna i mo`e se primenjivati na sve vrste uzoraka, {to pojednostavljuje dijagnostiku brojnih bo lesti i patolo{kih stanja. Posebna pa`nja u radu je posve }ena podacima iz literature koji ilustruju primenu Ramanske spektroskopije u transdermalnom monitoringu glukoze i dijagno stici kancera.

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“…It has been shown that bands assigned to localised vibrations of these bases exhibit pronounced intensity increases during DNA melting [21]. The peaks in this region which showed hyperchromic behaviour are 1340, 1487 and 1578 cm −1 representing purine bases and 1378 cm −1 representative of pyrimidine bases [8], [21] and [22]. A peak appeared at 1262 cm −1 , which is usually attributed to protonated cytosine, but the amount of plant extract used could not have lowered the pH to such an extent as to protonate cytosine [14].…”

Section: Impact Of Increasing Concentrations Of Ethanolic Extract Of mentioning

confidence: 99%

“…1003 sn Phenylalanine (ring breathing mode) [5], [6] and [10] 1031 mn Cell culture medium [7] 1096 mb DNA (O-P-O-stretch) [7] and [10] 1125 mn C-C stretching [5] 1172 wn Tyrosine, Phenylalanine [5] and [7] 1193-1207 mn Tyrosine, Phenylalanine [6] and [7] 1255-1268 wn Amide III [6] and [7] 1309 wn Adenine [14] 1340 wn C-H deformation, Tryptophan [6] and [7] Polynucleotide chain (DNA purine bases, specially Adenine) [5] and [10] 1361 mn Thymine and adenine [7] 1447 sb CH 2 stretching [6] and [7] CH 2 stretching [6] and [7] 1490 wn Adenine [11] 1555 mn Amide III (C-N stretching mode) [15] 1573-1580 mn Guanine, Adenine ring stretch [8] and [10] 1607 wn Phenylalanine and tyrosine [5] 1657 sb Amide I [16] C C stretch [6] Abbreviations regarding Raman peaks: s, strong; m, medium; w, weak; n, narrow; b, broad.…”

Section: Nucleic Acids Lipidsmentioning

confidence: 99%

A micro-Raman spectroscopic investigation of leukemic U-937 cells treated with Crotalaria agatiflora Schweinf and the isolated compound madurensine

Roux

Prinsloo

Hussein

et al. 2012

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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In South Africa traditional medicine plays an important role in primary health care and therefore it is very important that the medicinal use of plants is scientifically tested for toxicity and effectiveness. It was established that the ethanolic extract of the leaves of Crotalaria agatiflora, as well as the isolated compound madurensine, is moderately toxic against leukemic U-937 cells. The investigation showed that micro-Raman spectroscopy has great promise to be used for initial screening of samples to determine the effects of different treatments on cancerous cell lines together with conventional methods. The results highlight the fact that for many natural products used for medicinal purposes, the therapeutic effect of the crude plant extract tends to be significantly more effective than the particular action of its individual constituents. Graphical abstractLeukemic U-937 cells were treated with different concentrations of the ethanolic extract of Crotalaria agatiflora subspp. agatiflora and the isolated pyrrolizidine alkaloid, madurensine.Different biochemical responses were observed with micro-Raman spectroscopy after treatments. Highlights Crotalaria agatiflora Schweinf has an ethnobotanic use against cancer.  An ethanolic extract of the plant was tested against leukemic U-937 cells.  Raman spectra showed changes in cell morphology and biochemical constituents.  Crude plant extracts might be more bioactive than isolated compounds.  Raman spectroscopy has potential as an initial screening tool in phytomedicine.

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Mapping of single cells by near infrared Raman microspectroscopy

Cited by 171 publications

References 18 publications

Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus

Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus

Raman Spectroscopy as a New Biochemical Diagnostic Tool

A micro-Raman spectroscopic investigation of leukemic U-937 cells treated with Crotalaria agatiflora Schweinf and the isolated compound madurensine

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