Mapping of quantitative trait loci controlling barley flour pasting properties

Abstract

Pasting properties are important characteristics of barley starch from a processing standpoint. A shorter time to peak viscosity and lower pasting temperature are favorable to both malting and food processing. This study was conducted to identify quantitative trait loci (QTLs) determining pasting properties of barley flour using a doubled haploid population of 177 lines from the cross between six-rowed Yerong and two-rowed Franklin. Yerong is a feed barley with a longer time to peak viscosity and a higher pasting temperature than the other parent Franklin which is a malting barley. Field trials were conducted in three different sites/years. Seven different parameters representing the pasting properties were measured using a Rapid Visco-analyser (RVA). DH lines showed significant differences in all seven parameters in most of the sites/years. For example, the pasting temperature of different DH lines ranged from 73.8 to 89.5 in 2006/2007 MTP field trial. Twenty one QTLs were associated with flour pasting properties. These QTLs were distributed on 11 chromosome regions. Genetic variance explained by these QTLs varies from 4.4 to 15.2%. The most important QTLs controlling the time to peak viscosity and pasting temperature were located on 1H, 2H, 3H and 7H. Results showed that some of the pasting properties can be effectively selected by the combination of several molecular markers.

Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz)

“…For example, most QTL for rice starch quality co-localise with waxy (wx) and alkali degeneration (alk) genes (Bao et al 2003;Tian et al 2005). Wang et al (2010) identified 21 QTL associated with barley flour pasting properties and showed the potential of the markers for use in marker-assisted selection (MAS). It was found that SSIIa of maize affected starch structure and its resultant physiochemical properties, which is useful for a variety of applications that require freeze-thaw stability (Zhang et al 2004).…”

A regulator of early flowering in barley (Hordeum vulgare L.)

“…Flour pasting properties are important quality traits and have close relationship with malting quality [ 83 , 64 ] and food processing quality [ 65 ]. Pasting properties have been found to be influenced by genotype [ 83 , 84 ] and environment [ 65 , 84 – 86 ]. Earliness per se can also influence grain protein content [ 49 ] thus pasting properties [ 87 ].…”

“…Earliness per se can also influence grain protein content [ 49 ] thus pasting properties [ 87 ]. Several QTL have been identified for pasting properties in barley [ 86 ]. These QTL for pasting properties are located on chromosomes 1H, 2H, 3H, 4H, 6H and 7H [ 86 ] with no QTL on 5H, indicating that may be unlikely that this chromosome segment would affect pasting properties.…”

Identification of a Phytase Gene in Barley (Hordeum vulgare L.)

“…High N treatment showed significant reduction in phytase activity, which may be related to the higher average protein content (86.8 mg·g −1 ) caused by the higher rate of N application compared to the average protein content of 77.4 mg·g −1 where a low rate of N was applied. Samples from FVRS showed the highest phytase activity, which may be due to the low protein content of the grains [41]. The low phosphorus content in Forthside soil which has very high P fixing capacity could also cause an elevated phytase activity as phytase is used to maintain adequate available P for growth of the plants with P deficient [42].…”