Mapping of QTLs for androgenetic response based on a molecular genetic map of ×TriticosecaleWittmack

Abstract

Quantitative trait loci (QTLs) for androgenetic response were mapped in a doubled haploid (DH) population derived from the F1 hybrid of 2 unrelated varieties of triticale, 'Torote' and 'Presto'. A molecular marker linkage map of this cross was previously constructed using 73 DH lines. This map contains 356 markers (18 random amplified 5 polymorphic DNA, 40 random amplified microsatellite polymorphics, 276 amplified fragment length polymorphisms, and 22 simple sequence repeats) and was used for QTL analysis. The genome was well covered, and of the markers analysed, 336 were located in 21 linkage groups (81.9%) identified using SSR markers. The map covered a total length of 2465.4 cM with an average of 1 marker for each 6.9 cM. The distribution of the markers was not homogeneous across the 3 genomes, with 50.7% detected in the R genome. Several QTLs were found for the following variables related to the androgenetic response: number of embryos/100 anthers; plants regenerated from 100 embryos; number of green plants/total number of plants; and number of green plants/1000 anthers. Two were detected on chromosome 6B and 4R, which together had a 30% total influence on the induction of embryos. Another was found on 6B and on the unidentified LG1; these influenced the production of total plants from haploid embryo cultures. One QTL on chromosome 3R determined the photosynthetic viability of the haploid plantlets regenerated from microspores. Other QTLs were found on chromosomes 1B, 1R, 4R, and 7R, which helped the control of the final androgenetic response (the number of plantlets obtained for every 1000 anthers cultured).

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

“…Similar to reports in triticale, a low or insignificant correlation was found between green and albino plant production as well as regeneration (González et al 2005;Krzewska et al 2015). Opsahl-Ferstad et al (1994b) found an insignificant correlation of r = 0.29, similar to the ρ = 0.20 (P ≤ 0.05) found here, and hypothesized that different genes must be responsible for green and albino plant production.…”

Identification and validation of QTLs for green plant percentage in barley (Hordeum vulgare L.) anther culture

“…In this chromosome, no QTL associated with green plant percentage has been previously described in barley, wheat, or rye anther culture (Manninen 2000;Chen et al 2007;Torp et al 2001;Grosse et al 1996). However, a region of chromosome 3R has been recently associated with this trait in triticale anther culture (González et al 2005). In barley somatic embryogenesis, a QTL has been identified on chromosome 3H for green plant regeneration from callus (Qsr2, Mano et al 1996;Bregitzer and Campbell, 2001), although this QTL was located on the short arm of the chromosome.…”

“…QTLs for green plant percentage were mapped in wheat (Torp et al 2001 and2004), rye (Grosse et al 1996) and triticale (González et al 2005) anther culture. In barley, Manninen (2000) identified QTLs for anther culture response, although none of them was associated with green plant percentage.…”

In VitroResponse of Pepper Anther Culture (CapsicumannuumL.) Depending on Genotype, Nutrient Medium and Duration of Cultivation

“…Such homozygous DH plants have been used in breeding programmes for production of pure lines and new highly adaptive and productive varieties (1,4,8,15,16,18,20,21,23,24,35,41,48). A number of studies (2,19,37,45,53,55) also reported that the obtained by the in vitro anther culture techniques haploids and Dh plants are an appropriate system for genetic manipulations, gene mapping and mutant line selection.…”