Mapping of Leaf and Neck Blast Resistance Genes with RFLP, RAPD and Resistance Gene Analogs in Rice

Kangle, Zheng; Chai, Rongyao; Jin, Min-Zhong; Wu, Juan; Fan, Ye‐Yang; Leung, Hei; Zhuang, Jie-Yun

doi:10.1007/978-94-015-9430-1_4

Cited by 26 publications

(38 citation statements)

References 6 publications

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“…These findings are important to consider while testing cultivars under artificial epidemic conditions and before releasing lines as resources of resistance. Zhuang et al (25) provided evidence that, in rice, there is a genetic basis for the difference in response to leaf and neck blast resistance. In their study, they concluded that different blast resistance genes might be effective at different developmental stages.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Assessment of Resistance Among U.S. Wheat Cultivars to the Triticum Pathotype of Magnaporthe oryzae

et al. 2012

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Magnaporthe oryzae is the causal agent of blast disease on several graminaceous plants. The M. oryzae population causing wheat blast has not been officially reported outside South America. Wheat production in the United States is at risk to this pathogen if it is introduced and established. Proactive testing of U.S. wheat cultivars for their reaction to blast and identification of resistance resources is crucial due to the national and global importance of the U.S. wheat industry. In this preliminary study, the phenotypic reaction of 85 U.S. wheat cultivars to M. oryzae (Triticum pathotype) was determined. Although there was a significant correlation in the reaction to blast at the seedling and adult plant stages, only 57% of the head reaction was explained by the seedling reaction. Because of the importance of disease development at the head stage in the field, assessment of all 85 cultivars occurred at the head stage. Among cultivars tested, a continuum in severity to head blast was observed; cultivars Everest and Karl 92 were highly susceptible with more than 90% disease severity, while cultivars Postrock, JackPot, Overley, Jagalene, Jagger, and Santa Fe showed less than 3% infection. No evidence of the presence of physiological races among isolates T-7, T-12, T-22, and T-25 was found.

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Section: Discussionmentioning

confidence: 99%

Preliminary Assessment of Resistance Among U.S. Wheat Cultivars to the Triticum Pathotype of Magnaporthe oryzae

et al. 2012

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“…Some of the genes have been successfully used in blast resistance breeding. When a map-based cloning strategy is used in the identification of R genes, resistance gene analog (RGA) markers that are designed according to the conserved NBS domain could be used to identify the corresponding candidate NBS-LRR resistance gene (Mago et al 1999;Chauhan et al 2002;Zhuang et al 2002;Lee et al 2003;Sallaud et al 2003;Chen et al 2004). In practice, however, successful examples of isolation of an NBS-LRR resistance gene with RGA markers have rarely been reported.…”

Section: Discussionmentioning

confidence: 99%

Identification of a New Rice Blast Resistance Gene, Pid3, by Genomewide Comparison of Paired Nucleotide-Binding Site–Leucine-Rich Repeat Genes and Their Pseudogene Alleles Between the Two Sequenced Rice Genomes

et al. 2009

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Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. The two major subspecies of Asian cultivated rice (Oryza sativa L.), indica and japonica, have shown obvious differences in rice blast resistance, but the genomic basis that underlies the difference is not clear. We performed a genomewide comparison of the major class of resistant gene family, the nucleotide-binding site-leucinerich repeat (NBS-LRR) gene family, between 93-11 (indica) and Nipponbare ( japonica) with a focus on their pseudogene members. We found great differences in either constitution or distribution of pseudogenes between the two genomes. According to this comparison, we designed the PCR-based molecular markers specific to the Nipponbare NBS-LRR pseudogene alleles and used them as cosegregation markers for blast susceptibility in a segregation population from a cross between a rice blast-resistant indica variety and a susceptible japonica variety. Through this approach, we identified a new blast resistance gene, Pid3, in the indica variety, Digu. The allelic Pid3 loci in most of the tested japonica varieties were identified as pseudogenes due to a nonsense mutation at the nucleotide position 2208 starting from the translation initiation site. However, this mutation was not found in any of the tested indica varieties, African cultivated rice varieties, or AA genome-containing wild rice species. These results suggest that the pseudogenization of Pid3 in japonica occurred after the divergence of indica and japonica.

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“…Although mapping of over 25 major resistance genes and many quantitative trait loci, as well as the cloning of 2 resistance genes, has advanced our knowledge regarding the genetic mechanisms of disease resistance (Wang and Leung, 1998;Wang et al, 1999;Bryan et al, 2000, Zhuang et al, 2002, the molecular basis of the defense response to rice blast remains poorly understood. In addition to the genetic approach, a direct assessment of the biochemical and physiological changes during disease infection has been used to identify genes involved in defense pathways in many plants.…”

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Large-Scale Identification of Expressed Sequence Tags Involved in Rice and Rice Blast Fungus Interaction

et al. 2005

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To better understand the molecular basis of the defense response against the rice blast fungus (Magnaporthe grisea), a large-scale expressed sequence tag (EST) sequencing approach was used to identify genes involved in the early infection stages in rice (Oryza sativa). Six cDNA libraries were constructed using infected leaf tissues harvested from 6 conditions: resistant, partially resistant, and susceptible reactions at both 6 and 24 h after inoculation. Two additional libraries were constructed using uninoculated leaves and leaves from the lesion mimic mutant spl11. A total of 68,920 ESTs were generated from 8 libraries. Clustering and assembly analyses resulted in 13,570 unique sequences from 10,934 contigs and 2,636 singletons. Gene function classification showed that 42% of the ESTs were predicted to have putative gene function. Comparison of the pathogen-challenged libraries with the uninoculated control library revealed an increase in the percentage of genes in the functional categories of defense and signal transduction mechanisms and cell cycle control, cell division, and chromosome partitioning. In addition, hierarchical clustering analysis grouped the eight libraries based on their disease reactions. A total of 7,748 new and unique ESTs were identified from our collection compared with the KOME full-length cDNA collection. Interestingly, we found that rice ESTs are more closely related to sorghum (Sorghum bicolor) ESTs than to barley (Hordeum vulgare), wheat (Triticum aestivum), and maize (Zea mays) ESTs. The large cataloged collection of rice ESTs in this study provides a solid foundation for further characterization of the rice defense response and is a useful public genomic resource for rice functional genomics studies.

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Mapping of Leaf and Neck Blast Resistance Genes with RFLP, RAPD and Resistance Gene Analogs in Rice

Cited by 26 publications

References 6 publications

Preliminary Assessment of Resistance Among U.S. Wheat Cultivars to the Triticum Pathotype of Magnaporthe oryzae

Preliminary Assessment of Resistance Among U.S. Wheat Cultivars to the Triticum Pathotype of Magnaporthe oryzae

Identification of a New Rice Blast Resistance Gene, Pid3, by Genomewide Comparison of Paired Nucleotide-Binding Site–Leucine-Rich Repeat Genes and Their Pseudogene Alleles Between the Two Sequenced Rice Genomes

Large-Scale Identification of Expressed Sequence Tags Involved in Rice and Rice Blast Fungus Interaction

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