Mapping of cDNA clones on contig of Chlorella chromosome I

Maki, Sinya; Ohta, Yutaka; Noutoshi, Yoshiteru; Fujie, Makoto; Usami, Shohji; Yamada, Takashi

doi:10.1016/s1389-1723(01)80014-1

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…For example, early studies indicated that approximately 1.5% of its genome consists of LINE- and SINE-type retrotransposons [9,10]. Additional studies provided a detailed analysis of the smallest 980 kb chromosome [11,12]. …”

Section: Introductionmentioning

confidence: 99%

The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation

et al. 2012

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BackgroundLittle is known about the mechanisms of adaptation of life to the extreme environmental conditions encountered in polar regions. Here we present the genome sequence of a unicellular green alga from the division chlorophyta, Coccomyxa subellipsoidea C-169, which we will hereafter refer to as C-169. This is the first eukaryotic microorganism from a polar environment to have its genome sequenced.ResultsThe 48.8 Mb genome contained in 20 chromosomes exhibits significant synteny conservation with the chromosomes of its relatives Chlorella variabilis and Chlamydomonas reinhardtii. The order of the genes is highly reshuffled within synteny blocks, suggesting that intra-chromosomal rearrangements were more prevalent than inter-chromosomal rearrangements. Remarkably, Zepp retrotransposons occur in clusters of nested elements with strictly one cluster per chromosome probably residing at the centromere. Several protein families overrepresented in C. subellipsoidae include proteins involved in lipid metabolism, transporters, cellulose synthases and short alcohol dehydrogenases. Conversely, C-169 lacks proteins that exist in all other sequenced chlorophytes, including components of the glycosyl phosphatidyl inositol anchoring system, pyruvate phosphate dikinase and the photosystem 1 reaction center subunit N (PsaN).ConclusionsWe suggest that some of these gene losses and gains could have contributed to adaptation to low temperatures. Comparison of these genomic features with the adaptive strategies of psychrophilic microbes suggests that prokaryotes and eukaryotes followed comparable evolutionary routes to adapt to cold environments.

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Section: Introductionmentioning

confidence: 99%

The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation

et al. 2012

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Minichromosome formation in Chlorella cells irradiated with electron beams

Yamada¹,

Fujimoto²,

Yamamoto³

et al. 2003

Journal of Bioscience and Bioengineering

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Softening-up mannan-rich cell walls

Rodríguez-Gacio

Iglesias‐Fernández

Carbonero

et al. 2012

Journal of Experimental Botany

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The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the predominant hemicellulosic CW polymers in many genera of green algae. The endosperm CWs of dry seeds often contain mannan polymers, sometimes in the form of galactomannans (Gal-mannans). The endo-β-mannanases (MANs) that catalyse the random hydrolysis of the β-linkage in the mannan backbone are one of the main hydrolytic enzymes involved in the loosening and remodelling of CWs. In germinating seeds, the softening of the endosperm seed CWs facilitates the emergence of the elongating radicle. Hydrolysis and mobilization of endosperm Gal-mannans by MANs also provides a source of nutrients for early seedling growth, since Gal-mannan, besides its structural role, serves as a storage polysaccharide. Therefore, the role of mannans and of their hydrolytic enzymes is decisive in the life cycle of seeds. This review updates and discusses the significance of mannans and MANs in seeds and explores the increasing biotechnological potential of MAN enzymes.

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Mapping of cDNA clones on contig of Chlorella chromosome I

Cited by 3 publications

References 20 publications

The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation

The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation

Minichromosome formation in Chlorella cells irradiated with electron beams

Softening-up mannan-rich cell walls

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