Mapping Net Blotch Resistance in ‘Nomini’ and CIho 2291 Barley

Abstract

Net blotch (Pyrenophora teres) is one of the most devastating diseases of barley (Hordeum vulgare L.) worldwide. Identification of diagnostic molecular markers associated with genes and quantitative trait loci (QTL) for net blotch resistance will facilitate pyramiding of independent genes. Linkage mapping was used to identify chromosomal locations of the independent, dominant genes conditioning net blotch resistance in the winter barley ‘Nomini’ (PI 566929) and spring barley CIho 2291. The F2 populations of 238 and 193 individuals, derived from crosses between the susceptible spring barley parent ‘Hector’ (CIho 15514) and the resistant parents Nomini and CIho 2291, respectively, were used to map the genes governing resistance in the resistant parents. The dominant gene governing resistance in Nomini, temporarily designated Rpt‐Nomini, was mapped to a 9.2‐cM region of barley chromosome 6H between the flanking microsatellite markers Bmag0344a (r2 = 0.7) and Bmag0103a (r2 = 0.9), which were 6.8 and 2.4 cM away from Rpt‐Nomini, respectively. The dominant gene governing resistance in CIho 2291, temporarily designated Rpt‐CIho2291, was mapped to a 34.3‐cM interval on the distal region of barley chromosome 6H between the flanking microsatellite markers Bmag0173 (r2 = 0.65) and Bmag0500 (r2 = 0.26), which were 9.9 and 24.4 cM away from Rpt‐CIho2291, respectively. Identification of the chromosomal location of Rpt‐Nomini and Rpt‐CIho2291 will facilitate efforts in pyramiding multiple genes for net blotch resistance.

A nested association mapping population identifies multiple small effect QTL conferring resistance against net blotch (Pyrenophora teres f. teres) in wild barley

“… g Previously reported net blotch resistance QTL/genes located within the range of LD decay around the QTL marker with DR >50% identified in this study ( 1 reviewed in [ 4 ], 2 [ 17 ], 3 [ 18 ], 4 [ 25 ], 5 [ 19 ], 6 [ 26 ], 7 [ 21 ], 8 [ 70 ] …”

“…Positive and negative signs indicate a trait-increasing and trait-decreasing effect of the wild allele compared to the Barke control allele, respectively. g Previously reported net blotch resistance QTL/genes located within the range of LD decay around the QTL marker with DR >50% identified in this study ( 1 reviewed in [ 4 ], 2 [ 17 ], 3 [ 18 ], 4 [ 25 ], 5 [ 19 ], 6 [ 26 ], 7 [ 21 ], 8 [ 70 ] *for QTL defining the same position see [ 4 , 20 , 21 ]). …”

“…A good example is the centromeric region of chromosome 6H that is known to harbor a number of closely linked P . teres f. teres resistance genes of which some are assumed to be in repulsion [ 4 , 19 , 25 , 26 , 70 ]. Therefore, we assume that the high number of closely linked markers with opposed wild allele effect estimates in the centromeric region of chromosome 6H identified in this study is likely to be partially caused by this complex cluster of resistance related genes.…”

Genome-Wide Association Study for Spot Blotch Resistance in Hard Winter Wheat

“…Helminthosporium sativum , teleomorph Cochliobolus sativus ) is one of the destructive fungal diseases that affects wheat and several other small grains worldwide ( Dubin and Rajaram, 1996 ; Duveiller and Dubin, 2002 ; Joshi and Chand, 2002 ; Sharma et al, 2007b ; Singh and Singh, 2007 ; Gurung et al, 2009 ; Chowdhury et al, 2013 ). It has a wide range of hosts within wild and cultivated Poaceae species ( Kumar et al, 2002 ; Pandey et al, 2005 ; O’Boyle et al, 2014 ). In susceptible lines, SB symptoms are characterized by small, dark brown lesions that extend 1–2 mm long without chlorotic margins during initial infection ( Chand et al, 2003 ).…”

Research advances in the Pyrenophora teres–barley interaction

“…k) and Spt1.k (previously rpt5.r ) from CI5791/CI9819, Kombar and Rika, respectively (Abu Qamar et al , ; Koladia et al , ; Manninen et al , , ; Richards et al , ) (Table ). Additional loci that may also be part of the Rpt5 locus and therefore contain additional alleles include QRpts6L , QRpt6 , AL_QRptt6‐1, Rpt‐Nomini and Rpt‐CIho2291 in Halcyon (Raman et al , ), TR251 (Grewal et al , ), Lavrans (Wonneberger et al , ), Nomini and CIho2291 (O’Boyle et al , ), respectively (Table ), as well as undesignated QTLs from Steptoe (Steffenson et al , ), HOR 9088 (Richter et al , ), Kaputar, ND11213 (Cakir et al , ; Emebiri et al , ; Liu et al , ), Chevron (Ma et al , ), SM89010 (Friesen et al , ), M129 (St. Pierre et al , ), Baudin (Cakir et al , ), WPG8412, Pompadour, Stirling (Gupta et al , ), Falcon (Islamovic et al , ), H602 (Hisano et al , ) and UVC8 (Martin et al , ) (Table ).…”