Mapping molecules to structure: unveiling secrets of centriole and cilia assembly with near-atomic resolution

Jana, Swadhin Chandra; Marteil, Gaëlle; Bettencourt-Dias, Mónica

doi:10.1016/j.ceb.2013.12.001

Cited by 61 publications

(56 citation statements)

References 88 publications

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“…New insights into centriole biogenesis will greatly enhance our understanding of the orchestration of the centrosome cycle and of mitotic spindle formation. As a number of excellent reviews on centriole biogenesis have been published recently (Avidor-Reiss and Gopalakrishnan, 2013;Gönczy, 2012;Jana et al, 2014), here, we mainly discuss how mitotic kinases function in centrosome duplication and summarize the general aspects of this process (see Table 1). …”

Section: The Role Of Mitotic Kinases In Centriole Biogenesismentioning

confidence: 99%

The role of mitotic kinases in coupling the centrosome cycle with the assembly of the mitotic spindle

Wang

Jiang

Zhang

2014

Journal of Cell Science

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The centrosome acts as the major microtubule-organizing center (MTOC) for cytoskeleton maintenance in interphase and mitotic spindle assembly in vertebrate cells. It duplicates only once per cell cycle in a highly spatiotemporally regulated manner. When the cell undergoes mitosis, the duplicated centrosomes separate to define spindle poles and monitor the assembly of the bipolar mitotic spindle for accurate chromosome separation and the maintenance of genomic stability. However, centrosome abnormalities occur frequently and often lead to monopolar or multipolar spindle formation, which results in chromosome instability and possibly tumorigenesis. A number of studies have begun to dissect the role of mitotic kinases, including NIMA-related kinases (Neks), cyclindependent kinases (CDKs), Polo-like kinases (Plks) and Aurora kinases, in regulating centrosome duplication, separation and maturation and subsequent mitotic spindle assembly during cell cycle progression. In this Commentary, we review the recent research progress on how these mitotic kinases are coordinated to couple the centrosome cycle with the cell cycle, thus ensuring bipolar mitotic spindle fidelity. Understanding this process will help to delineate the relationship between centrosomal abnormalities and spindle defects.

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Section: The Role Of Mitotic Kinases In Centriole Biogenesismentioning

confidence: 99%

The role of mitotic kinases in coupling the centrosome cycle with the assembly of the mitotic spindle

Wang

Jiang

Zhang

2014

Journal of Cell Science

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“…The convergence of genetic and proteomic analyses with structural imaging of mutants has enabled the dissection of many of the stages of centriole biogenesis (reviewed in Brito et al, 2012;Gö nczy, 2012;Jana et al, 2014;Dutcher, 2007). The site of new centriole formation is defined by the Polo-like kinase, PLK4, and its recruitment factors, CEP152 and CEP192, near the wall of the mother centriole (M), which precedes cartwheel formation (see figure).…”

Section: Nascent Cbbs Form Near the Mother Cbbmentioning

confidence: 99%

“…The proximal ends of CBBs are capped with the cartwheel, and the distal ends are capped with centrin and the CP110 protein, which limits centriole length. Gönczy, 2012;Jana et al, 2014). The close proximity of the assembly of the daughter CBB to the mother structure led to the term 'templated' duplication, suggesting that the mother CBB somehow specifies the organization of the daughter.…”

Section: Nascent Cbbs Form Near the Mother Cbbmentioning

confidence: 99%

Choosing sides – asymmetric centriole and basal body assembly

Pearson

2014

Journal of Cell Science

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Centrioles and basal bodies (CBBs) are microtubule-rich cylindrical structures that nucleate and organize centrosomes and cilia, respectively. Despite their apparent ninefold rotational symmetry, the nine sets of triplet microtubules in CBBs possess asymmetries in their morphology and in the structures that associate with them. These asymmetries define the position of nascent CBB assembly, the orientation of ciliary beating, the orientation of spindle poles and the maintenance of cellular geometry. For some of these functions, the orientation of CBBs is first established during new CBB biogenesis when the daughter structure is positioned adjacent to the mother. The mother CBB organizes the surrounding environment that nascent CBBs are born into, thereby providing a nest for the new CBB to develop. Protists, including ciliates and algae, highlight the importance of this environment with the formation of asymmetrically placed scaffolds onto which new basal bodies assemble and are positioned. Recent studies illuminate the positioning of nascent centrioles relative to a modular pericentriolar material (PCM) environment and suggest that, like ciliates, centrosomes organize an immediate environment surrounding centrioles for their biogenesis and positioning. In this Commentary, I will explore the positioning of nascent CBB assembly as the first event in building cellular asymmetries and describe how the environment surrounding both basal bodies and centrioles may define asymmetric assembly.

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“…In addition to their role in nucleating centrosome assembly, fully mature centrioles also function as basal bodies for the formation of cilia and flagella (Ishikawa & Marshall, 2011; Bornens, 2012). Numerical and structural aberrations in centrioles and centrosomes have been implicated in human disease, including cancer, microcephaly, dwarfism, and ciliopathies (Nigg & Raff, 2009; Zyss & Gergely, 2009; Bettencourt‐Dias et al , 2011; Ishikawa & Marshall, 2011; Chavali et al , 2014; Jana et al , 2014; Gonczy, 2015). …”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

et al. 2016

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Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly, and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas‐6, and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes is presently known. Here, we have used two complementary approaches, targeted proteomics and EGFP‐tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. Specifically, they predict that human centriolar cartwheels comprise up to 16 stacked hubs and 1 molecule of STIL for every dimer of Sas‐6. This type of quantitative information will help guide future studies of the molecular basis of centrosome assembly and function.

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Mapping molecules to structure: unveiling secrets of centriole and cilia assembly with near-atomic resolution

Cited by 61 publications

References 88 publications

The role of mitotic kinases in coupling the centrosome cycle with the assembly of the mitotic spindle

The role of mitotic kinases in coupling the centrosome cycle with the assembly of the mitotic spindle

Choosing sides – asymmetric centriole and basal body assembly

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

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