Mapping Maize Sequences to Chromosomes Using Oat-Maize Chromosome Addition Materials,

Okagaki, Ron J.; Kynast, Ralf G.; Livingston, Suzanne M.; Russell, Charles D.; Rines, H. W.; Phillips, Ronald L.

doi:10.1104/pp.125.3.1228

Cited by 51 publications

(30 citation statements)

References 26 publications

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“…Using wheat-barley chromosome addition lines, isozymes and DNA markers have been physically mapped to chromosomes and chromosome arms (e.g., Islam and Shepherd 1990;Garvin et al 1998). Similar studies have been conducted in a set of oat-maize chromosome addition lines and their radiation hybrids (Okagaki et al 2001;Kynast et al 2004). Although the use of addition lines for physical mapping in both systems has been successful, the use of these lines as high-throughput mapping resources has been restricted by the lack of technologies to allocate markers to specific chromosomes.…”

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confidence: 97%

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Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

2006

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Wheat-barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese SpringBetzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe array. Of the 4014 transcripts detected in Betzes but not in Chinese Spring, 365, 271, 265, 323, 194, and 369 were detected in wheat-barley disomic chromosome addition lines 2(2H), 3(3H), 4(4H), 7(5H), 6(6H), and 1(7H), respectively. Thus, 1787 barley transcripts were detected in a wheat genetic background and, by virtue of the addition line in which they were detected, were physically mapped to barley chromosomes. We validated and extended our approach to physically map barley genes to the long and short arms of chromosome 6(6H). Our physical map data exhibited a high level of synteny with homologous sequences on the wheat and/or rice syntenous chromosomes, indicating that our barley physical maps are robust. Our results show that barley transcript detection in wheat-barley chromosome addition lines is an efficient approach for large-scale physical mapping of genes.

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confidence: 97%

“…The oat-maize chromosome addition lines were used to assign 300 EST and 50 STS markers to maize chromosomes (Okagaki et al 2001). In wheat, using 101 wheat chromosome deletion lines, 24 ditelosomic lines, and 21 nullisomic-tetrasomic lines, 7873 unique ESTs were mapped to 21 chromosomes in three genomes (Akhunov et al 2003; http://wheat.pw.…”

Section: )mentioning

confidence: 99%

Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

2006

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“…For example, Okagaki et al (32) mapped 350 ESTs and sequence tagged sites to chromosomes by a presence vs. absence test and ), arrow points to the deficient short arm of maize chromosome 1; the chromosome lost Ϸ20% of its short arm. p-umc1626 is the most distal present marker tested (see also Fig.…”

Section: Discussionmentioning

confidence: 99%

Dissecting the maize genome by using chromosome addition and radiation hybrid lines

Kynast

Okagaki

Galatowitsch

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is available only in haploid oat background. Most of the maize chromosome disomic addition lines have regular transmission; however, chromosome 5 showed diminished paternal transmission, and chromosome 10 is transmitted to offspring only as a short-arm telosome. To further dissect the maize genome, we irradiated monosomic additions with ␥ rays and recovered radiation hybrid (RH) lines providing low-to medium-resolution mapping for most of the maize chromosomes. For maize chromosome 1, mapping 45 simple-sequence repeat markers delineated 10 groups of RH plants reflecting different chromosome breaks. The present chromosome 1 RH panel dissects this chromosome into eight physical segments defined by the 10 groups of RH lines. Genomic in situ hybridization revealed the physical size of a distal region, which is represented by six of the eight physical segments, as being Ϸ20% of the length of the short arm, representing Ϸone-third of the genetic chromosome 1 map. The distal Ϸ20% of the physical length of the long arm of maize chromosome 1 is represented by a single group of RH lines that spans >23% of the total genetic map. These oat-maize RH lines provide valuable tools for physical mapping of the complex highly duplicated maize genome and for unique studies of interspecific gene interactions. P lants with one chromosome (monosomic) or one pair of homologous chromosomes (disomic) of an alien donor species added to the entire recipient species chromosome complement serve to dissect the donor genome into individual chromosome entities and separate them from their own genome remnant. The transfer liberates the added chromosome (pair) from the interactive gene expression network of the donor genome and puts the chromosome's genes into the environment of the host genome. This new structural and functional situation can create novel orthologous and nonhomologous gene-to-gene interactions and, hence, helps to answer fundamental questions about gene expression control, inheritance, and syntenic correspondence among different plant species, especially those with large genomes, including maize, with a 1C content of Ϸ2. By crossing maize to oat, (oat ϫ maize)F 1 proembryos were generated, of which 5-10% could be rescued in vitro. Molecular and cytological analyses showed retention of one or more maize chromosomes in addition to the haploid oat genome in 34% of the F 1 plants (2-7). Because haploid oat frequently develops unreduced gametes (8), subsequent self-fertilization of (oat ϫ maize)F 1 plants with one maize chromosome added to the haploid oat genome (n ϭ 3x ϩ 1 ϭ 22) can produce F 2 offspring with one homologous maize chromosome pair added to the doubled haploid (hexaploid) oat genome (2n ϭ 6x ϩ 2 ϭ 44) among other euploid and aneuploid types (9).A complete series of oat-maize chromosome a...

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“…Hml was isolated from a genomic library of B73 as described (28). Hml was mapped to the maize chromosome 7 by using oat-maize addition lines DNA (29). The barley Hm1 homolog was cloned by RT-PCR from total RNA isolated from Black Hulless seedling as described above.…”

Section: Vigs Experimentsmentioning

confidence: 99%

A guardian of grasses: Specific origin and conservation of a unique disease-resistance gene in the grass lineage

Sindhu

Chintamanani

Brandt

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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The maize Hm1 gene provides protection against a lethal leaf blight and ear mold disease caused by Cochliobolus carbonum race 1 (CCR1). Although it was the first disease-resistance (DR) gene to be cloned, it remains a novelty because, instead of participating in the plant recognition and response system as most DR genes do, Hm1 disarms the pathogen directly. It does so by encoding an NADPH-dependent reductase, whose function is to inactivate Helminthosporium carbonum (HC) toxin, an epoxide-containing cyclic tetrapeptide, which the pathogen produces as a key virulence factor to colonize maize. Although CCR1 is strictly a pathogen of maize, orthologs of Hm1 and the HC-toxin reductase activity are present in the grass family, suggesting an ancient and evolutionarily conserved role of this DR trait in plants. Here, we provide proof for such a role by demonstrating its involvement in nonhost resistance of barley to CCR1. Barley leaves in which expression of the Hm1 homologue was silenced became susceptible to infection by CCR1, but only if the pathogen was able to produce HC toxin. Phylogenetic analysis indicated that Hm1 evolved exclusively and early in the grass lineage. Given the devastating ability of CCR1 to kill maize, these findings imply that the evolution and/or geographical distribution of grasses may have been constrained if Hm1 did not emerge.host-pathogen specificity ͉ nonhost resistance ͉ host-specific toxin ͉ Cochliobolus ͉ Helminthosporium carbonum toxin reductase

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Mapping Maize Sequences to Chromosomes Using Oat-Maize Chromosome Addition Materials,

Cited by 51 publications

References 26 publications

Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

Dissecting the maize genome by using chromosome addition and radiation hybrid lines

A guardian of grasses: Specific origin and conservation of a unique disease-resistance gene in the grass lineage

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