Mapping Local and Systemic Symptom Determinants of Cucumber Mosaic Cucumovirus in Tobacco

Zhang, Lee; Hanada, Kaoru; Palukaitis, Peter

doi:10.1099/0022-1317-75-11-3185

Cited by 112 publications

(93 citation statements)

References 14 publications

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“…2a) could be used to differentiate subgroup I strains (severe symptoms) from subgroup II strains (mild symptoms), similar to the observations of CMV strains systemically infecting tobacco (Zhang et al 1994). Using pseudorecombinants, these authors concluded that CMV RNAs 1 and 2 were involved in determining symptom severity.…”

Section: Discussionsupporting

confidence: 55%

“…Maize appears to be resistant to systemic infection by most subgroup II strains (Wahyuni et al 1992;Carrère et al 1999;Ryu et al 1998). CMV sequences that control symptom type and virulence have been mapped to the 3a and CP genes on RNA 3 (Carrère et al 1999;Suzuki et al 1995;Sugiyama et al 2000;Szilassy et al 1999), but RNAs 1 and 2 have also been implicated (Roossinck and Palukaitis 1990;Zhang et al 1994;Hellwald et al 2000b;Soards et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…CMV infection causes a broad spectrum of symptoms from mild to severe mosaic, stunting, filiformism, chlorosis and necrosis, depending on virus strain and host (Carrère et al 1999). In tobacco, CMV strains belonging to subgroup I tend to cause severe mosaic whereas subgroup II strains lead to mild mosaic (Wahyuni et al 1992;Zhang et al 1994). Some subgroup I strains cause fern-shaped or filiform leaves in tomato (Hellwald et al 2000b;Stamova and Chetelat 2000).…”

Section: Introductionmentioning

confidence: 99%

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Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Sulistyowati

Mitter²,

Bastiaan‐Net³

et al. 2004

Austral. Plant Pathol.

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G The first two authors contributed equally to this work.Abstract. We have characterised six Australian Cucumber mosaic virus (CMV) strains belonging to different subgroups, determined by the sequence of their complete RNA 3 and by their host range and the symptoms they cause on species in the Solanaceae, Cucurbitaceae and on sweet corn. These data allowed classification of strains into the known three CMV subgroups and identification of plant species able to differentiate the Australian strains by symptoms and host range. Western Australian strains 237 and Twa and Queensland strains 207 and 242 are closely related members of CMV subgroup IA, which cause similar severe symptoms on Nicotiana species. Strains 207 and 237 (subgroup IA) were the only strains tested which systemically infected sweet corn. Strain 243 caused the most severe symptoms of all strains on Nicotiana species, tomato and capsicum and appears to be the first confirmed subgroup IB strain reported in Australia. Based on pair-wise distance analysis and phylogeny of RNA 3, as well as mild disease symptoms on Nicotiana species, CMV 241 was assigned to subgroup II, as the previously described Q-CMV and LY-CMV.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Sulistyowati

Mitter²,

Bastiaan‐Net³

et al. 2004

Austral. Plant Pathol.

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“…Depending on the host and virus strain, CMV is known to cause variable symptoms, including necrotic or chlorotic lesions, mild to severe mosaic, stunting, leaf deformation and shoestring formation [14]. Subgroup I strains shows severity in terms of symptom and disease development on tobacco [55]. The present isolate (CMV-SG) induced chlorotic lesions becoming necrotic in local lesion assay host, Chenopodium quinoa 3 dpi.…”

Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India.

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“…The generation of cDNA clones, which contained either the wild-type (wt) 3a gene and the CP gene replaced by the gene encoding the GFP (pL:3a/GFP), or just the 3a gene replaced by the gene encoding the GFP (pF:GFP/CP), was described previously (Canto et al, 1997). RNA transcripts from any of the above plasmids were generated using T7 RNA polymerase, as described previously (Zhang et al, 1994).…”

Section: Methodsmentioning

confidence: 99%

The C-terminal 33 amino acids of the cucumber mosaic virus 3a protein affect virus movement, RNA binding and inhibition of infection and translation

Kim¹,

Калинина

Андреев

et al. 2004

Journal of General Virology

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The capsid protein (CP) of Cucumber mosaic virus (CMV) is required for cell-to-cell movement, mediated by the 3a movement protein (MP). Deletion of the C-terminal 33 amino acids of the CMV 3a MP (in the mutant designated 3aDC33 MP) resulted in CP-independent cell-to-cell movement, but not long-distance movement. RNA-binding studies done in vitro using isolated bacterially expressed MP showed that the 3aDC33 MP bound RNA more strongly, with fewer regions sensitive to RNase and formed cooperatively bound complexes at lower ratios of protein : RNA than the wild-type (wt) 3a MP. Analysis of the architecture of the complexes by atomic force microscopy showed that the wt 3a MP formed a single type of complex with RNA, resembling beads on a string. By contrast, the 3aDC33 MP formed several types of complexes, including complexes with virtually no MP bound or thicker layers of MP bound to the RNA. Assays showed that protein-RNA complexes containing high levels of either MP inhibited the infectivity and in vitro translatability of viral RNAs. The 3aDC33 MP inhibited these processes at lower ratios of protein : RNA than the wt 3a MP, consistent with its stronger binding properties. The apparent contradiction between these inhibition data and the CP-independent cell-to-cell movement of CMV expressing the 3aDC33 MP is discussed.

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Mapping Local and Systemic Symptom Determinants of Cucumber Mosaic Cucumovirus in Tobacco

Cited by 112 publications

References 14 publications

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

The C-terminal 33 amino acids of the cucumber mosaic virus 3a protein affect virus movement, RNA binding and inhibition of infection and translation

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