Abstract:The emergence of Do-It-Yourself (DIY) science movements is becoming a topic widely discussed in academia and policy, as well as by the general public and the media. While DIY approaches enjoy increasing diffusion even in official research, different social actors frequently talk about them in different ways and circumstances. Interaction and negotiation processes amongst actors (e.g. policy makers and DIY communities) define the premises upon which different conceptualisations of DIY science are deployed.In th… Show more
“…They associate the DIYBM most frequently with other broader, current movements. The most common associated movements are the “quantified self” and “self‐experimentalists” (Yetisen, ), “open source” (Meyer, ), and “open science” (Aungst, Fishman, & McGowan, ), “action research” (Golinelli & Ruivenkamp, ), “synthetic biology” (Bennett, Gilman, Stavrianakis, & Rabinow, ), “maker” (Tocchetti, ), “hacker” (Meyer, ),“citizen science” (Keulartz & van den Belt, ) and “DIY science” (Ferretti, ). The movements are usually associated in terms of similarities in ethics, politics, economics, and practices.…”
Section: Resultsmentioning
confidence: 99%
“…According to Chen and Wu (), the DIY movement is closely linked to the physical activity of making which is the key trait of the maker movement. Ferretti (, p. 14) argues that everyone “that operates creative skills to design and make objects, as well as applies peer‐to‐peer based learning to solve problems” is a DIY maker. In this sense, the protagonists of the DIYBM are makers because they use their creative skills to design and make biological objects (Keulartz & van den Belt, ).…”
Section: Resultsmentioning
confidence: 99%
“…DIY science movement . For Ferretti (, p. 4) citizen science refers to a “collective scientific practice”, while DIY science refers to the scientific “process initiated by individuals and groups that tinker, hack, fix, and recreate objects and systems out of their own interest, curiosity or need, and openly share results and outcomes in their networks”. In this sense, the DIYBM is a sub movement of DIY science focused on biological objects and systems.…”
Human enhancement aims at improving individual human performance through science‐based or technology‐based interventions in the human body. For various decades, associated research and applications/interventions were performed in conventional settings (e.g., research institutes) through conventional regulated and controlled procedures (e.g., clinical trials). In the last decade there has been an emergence of science activities grounded on emerging technologies used in unconventional settings (e.g., households; community labs), often through unconventional unregulated and uncontrolled procedures (e.g., self‐administration of substances). The Do‐It‐Yourself Biology or Biohacking movement is an example of communities supportive of such activities, which use emerging technologies such as the CRISPR technique. Among others, these can have other or self‐enhancement goals. Because such activities are anticipated to increase in the future, and due to the methods novelty, lack of regulation, quality, and safety control, there is uncertainty regarding personal and social consequences. Thus, these can be considered to present an emerging risk to human health and the environment. A first step in risk regulation is considering ethical aspects of emerging technologies use, which has been implemented. A second step to sustain subsequent evidence‐based risk management and risk communication to citizen scientists, is necessary. It should involve risk assessment by experts and an understanding of public views on human enhancement technologies. Due to the scarce literature, gathering information to support this step was the goal of a non‐systematic literature review. This focused on internal enhancements through substances intake and human body manipulations, specifically DIY biology/biohacking activities with this goal.
“…They associate the DIYBM most frequently with other broader, current movements. The most common associated movements are the “quantified self” and “self‐experimentalists” (Yetisen, ), “open source” (Meyer, ), and “open science” (Aungst, Fishman, & McGowan, ), “action research” (Golinelli & Ruivenkamp, ), “synthetic biology” (Bennett, Gilman, Stavrianakis, & Rabinow, ), “maker” (Tocchetti, ), “hacker” (Meyer, ),“citizen science” (Keulartz & van den Belt, ) and “DIY science” (Ferretti, ). The movements are usually associated in terms of similarities in ethics, politics, economics, and practices.…”
Section: Resultsmentioning
confidence: 99%
“…According to Chen and Wu (), the DIY movement is closely linked to the physical activity of making which is the key trait of the maker movement. Ferretti (, p. 14) argues that everyone “that operates creative skills to design and make objects, as well as applies peer‐to‐peer based learning to solve problems” is a DIY maker. In this sense, the protagonists of the DIYBM are makers because they use their creative skills to design and make biological objects (Keulartz & van den Belt, ).…”
Section: Resultsmentioning
confidence: 99%
“…DIY science movement . For Ferretti (, p. 4) citizen science refers to a “collective scientific practice”, while DIY science refers to the scientific “process initiated by individuals and groups that tinker, hack, fix, and recreate objects and systems out of their own interest, curiosity or need, and openly share results and outcomes in their networks”. In this sense, the DIYBM is a sub movement of DIY science focused on biological objects and systems.…”
Human enhancement aims at improving individual human performance through science‐based or technology‐based interventions in the human body. For various decades, associated research and applications/interventions were performed in conventional settings (e.g., research institutes) through conventional regulated and controlled procedures (e.g., clinical trials). In the last decade there has been an emergence of science activities grounded on emerging technologies used in unconventional settings (e.g., households; community labs), often through unconventional unregulated and uncontrolled procedures (e.g., self‐administration of substances). The Do‐It‐Yourself Biology or Biohacking movement is an example of communities supportive of such activities, which use emerging technologies such as the CRISPR technique. Among others, these can have other or self‐enhancement goals. Because such activities are anticipated to increase in the future, and due to the methods novelty, lack of regulation, quality, and safety control, there is uncertainty regarding personal and social consequences. Thus, these can be considered to present an emerging risk to human health and the environment. A first step in risk regulation is considering ethical aspects of emerging technologies use, which has been implemented. A second step to sustain subsequent evidence‐based risk management and risk communication to citizen scientists, is necessary. It should involve risk assessment by experts and an understanding of public views on human enhancement technologies. Due to the scarce literature, gathering information to support this step was the goal of a non‐systematic literature review. This focused on internal enhancements through substances intake and human body manipulations, specifically DIY biology/biohacking activities with this goal.
“…However, it is increasingly acknowledged that there are ‘many modes of citizen science’ (Kasperowski and Kullenberg, 2019), coming along under terms such as ‘grassroots’ science, ‘civic’ science or ‘community-based’ research, and some of them are grounded, at least originally, outside institutional science. Even more fuzzy and difficult to grasp is the emerging science-related practices by citizens and users that have become known as maker movement, bio-hacking, do-it-yourself (DIY) biology or DIY science (Delfanti, 2010; Delgado and Callén, 2017; Ferretti, 2019). Self-tracking research adds to these diverse and complex science-related practices that challenge the familiar ways of conceptualising the science-society relationship.…”
This article explores the production and type of knowledge acquired in the course of specific digital self-tracking activities that resemble research and are common among followers of the Quantified Self movement. On the basis of interviews with self-trackers, it is shown that this knowledge can be characterised as a verified and practical self-knowledge, and that science in the form of scientific sources, methods and quality criteria plays a key role in its production. It is argued that this self-related knowledge can be conceptualised as self-expertise, and its production as personal science. The article then discusses the implications for the science-society relationship. In contrast to self-tracking data, so far self-knowledge has hardly caused any resonance in science, although science currently appears open to the insights from single subject (N-of-1) research. As a new mode of public engagement with science, personal science instead mainly leads to an individual self-expertisation.
“…Although there are examples of cost-effective devices, for example, polydimethylsiloxane (PDMS)- or alginate-based microfluidic systems [14,15,16,17,18,19] which allow for automated liquid handling, as well as three-dimensional (3D) printed chambers for maintenance and microscopic observation of cultured cells [20,21], a platform for chemical stimulation and parallel analysis of ion channel function that is reproducible within a short time, scalable to higher throughput screening mode and at the same time an ultra-low-cost device, has not yet been reported. The increasing availability of rapid manufacturing technology, including 3D printing, also adopted by so-called fabrication labs and the “maker movement”, a culture of do-it-yourself (DIY) system design [22,23,24,25,26], allows virtually anyone to quickly and easily engineer devices of reduced cost and complexity.…”
Functional imaging has been a widely established method for the assessment of ion channel function in vitro. Conventional infrastructure used for in vitro functional analysis of ion channels is typically proprietary, non-customizable, expensive, and requires a high level of skill to use and maintain. 3D desktop printing, which is employed in the rapid prototyping field, allows for quick engineering of alternatives to conventional imaging infrastructure that are customizable, low cost, and user friendly. Here, we describe an ultra-low-cost microfluidic lab-on-a-chip (LOC) device manufactured using acrylonitrile butadiene styrene (ABS) for in vitro functional imaging of ion channels that can quickly and easily be reconstructed using three-dimensional (3D) desktop printing. The device is light weight (<5 g), small (20 mm × 49 mm), and extremely low cost (<EUR 1). We simulate fluidics within the printed channels and assess the suitability of the engineered chamber to generate homogeneous mixtures during solution exchange. We demonstrate the usability of the 3D printed microfluidic device in a case study using Fluo-4-loaded human embryonal kidney-derived (HEK293) cells, recombinantly expressing the capsaicin receptor, transient receptor potential vanilloid receptor type 1 (TRPV1), as a model system. In the case study, we confirm its applicability to solution exchange for chemical stimulation and parallel functional time-lapse fluorescence microscopy-based calcium imaging. We assess the suitability of ABS for culturing HEK293 cells inside the microfluidic LOC, based on qualitative analysis of microscopic transmission light images of ABS-exposed HEK293 cells and confirm the previously reported biocompatibility of ABS. To highlight the versatility of the 3D printed microfluidic device, we provide an example for multiplication of the shown concept within a 3D printed multichannel microfluidic LOC to be used, for example, in a higher throughput format for parallelized functional analysis of ion channels. While this work focusses on Ca2+ imaging with TRPV1 channels, the device may also be useful for application with other ion channel types and in vitro models.
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