Mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the gE gene from HSV-1

Rebordosa, Xavier; Piñol, Jaume; Pérez-Pons, Josep A.; Lloberas, Jorge; Naval, Jordi; Querol, Enrique

doi:10.1016/0378-1119(94)90151-1

Cited by 17 publications

(16 citation statements)

References 33 publications

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“…Two of these glycoproteins, gE and gI, have been shown to be important for virulence and spread of several herpesviruses in all animal models tested (2, 9-11, 27, 33, 34, 39, 52, 55). Although dispensable for replication in cultured cells, the genes for gE and gI are conserved in all alphaherpesviruses studied to date (3,19,37,39,48). The gE and gI proteins in alphaherpesviruses associate with each other soon after synthesis and form a noncovalent hetero-oligomeric complex (28,29,55,56,60).…”

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confidence: 99%

Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit

Chowdhury¹,

Lee²,

Özkul³

et al. 2000

J Virol

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Glycoprotein E (gE) is important for full virulence potential of the alphaherpesviruses in both natural. Virus isolation and immunohistochemistry data suggest that these recombinants replicate and spread significantly less efficiently in the brain than BHV-5 gE revertant or wild-type BHV-5, which produced severe neurological signs in 70 to 80% rabbits. Taken together, the results of neurological signs, brain lesions, virus isolation, and immunohistochemistry indicate that BHV-5 gE is important for efficient neural spread and neurovirulence within the CNS and could not be replaced by BHV-1 gE. However, BHV-5 gE is not required for initial viral entry into olfactory pathway.

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confidence: 99%

Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit

Chowdhury¹,

Lee²,

Özkul³

et al. 2000

J Virol

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“…Therefore, we developed an autochtonous gE -BoHV-1 so that, with the use of an anti-gE serological test, the immune response to the vaccinal virus can be differentiated from that induced by wild type virus, and yet retaining most of the antigenic characteristics common to local BoHV-1 isolates. Other gE -bovine herpesviruses have been described (1,11,15,18), however, this is the first report on the introduction of a whole gE deletion on a BoHV-1.2 strain. An option was made for a BoHV-1.2a in view of the fact that isolates of this subtype not only may cause respiratory disease but may also be involved in reproductive disorders, causing miscarriage (3,15).…”

Section: Discussionmentioning

confidence: 89%

“…Previous studies were performed to access the in vitro behavior of gE -BoHV-1.1 mutants (1,11). In order to determine the in vitro growth behavior of the gE -BoHV-1.2 and to make comparative analysis between BoHV-1.1 and BoHV-1.2, growth kinetics and viral plaque size of viruses will be determined.…”

Section: Discussionmentioning

confidence: 99%

“…The viral genome encodes approximately 70 different proteins, of which eleven are glycoproteins. One of these glycoproteins, glycoprotein E (gE), is encoded by a gene located within the US region (11,17,18). Glycoprotein E is not essential for viral replication, but in vitro growth analysis of viral mutants show a decreased cell-to-cell spread and smaller plaque size for gE negative viruses (11,17).…”

Section: Introductionmentioning

confidence: 99%

“…One of these glycoproteins, glycoprotein E (gE), is encoded by a gene located within the US region (11,17,18). Glycoprotein E is not essential for viral replication, but in vitro growth analysis of viral mutants show a decreased cell-to-cell spread and smaller plaque size for gE negative viruses (11,17). In addition, gE deletion in vivo has been related to a decreased virulence in calves (1,14,15).…”

Section: Introductionmentioning

confidence: 99%

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Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

Franco¹,

Rijsewijk²,

Flores³

et al. 2002

Braz. J. Microbiol.

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This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by cotransfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine.

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Epitopes on glycoprotein E and on the glycoprotein E/glycoprotein I complex of bovine herpesvirus 1 are expressed by all of 222 isolates and 11 vaccine strains

Rijsewijk¹,

Kaashoek²,

Langeveld³

et al. 2000

Arch. Virol.

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Glycoprotein E (gE) of bovine herpesvirus 1 (BHV1) forms a complex with glycoprotein I (gI) and plays an important role in cell-to-cell spread mechanisms of the virus, but is not essential for propagation of the virus. To study the antigenic variability of BHV1 glycoprotein E, a set of six well characterised monoclonal antibodies (MAbs) was established using BHV1 gE and gI deletion mutants, eukaryotically expressed gE and gI and pepscan analysis. Two of these MAbs reacted with a linear gE epitope (MAbs 3 and 52), two reacted with a more conformation dependent gE epitope (MAbs 61 and 81) and two reacted with epitopes formed by a complex formed between gE and glycoprotein I (MAbs 67 and 75). With these six MAbs the gE expression of 222 BHV1 isolates and 11 BHV1 modified-live vaccine strains was studied in vitro, using an immunoperoxidase monolayer assay. All 222 BHV1 isolates and 11 vaccine strains were found to react with MAbs 61, 81 and 75. Three of the 222 isolates failed to react with MAb 67 and two of the vaccines reacted very weakly with MAbs 3 and 52. Analysis of the gE genes of these five aberrant isolates and the gE glycoproteins they expressed, did not show obvious size differences compared to wild-type BHV1. We conclude that the tested gE epitopes are highly conserved, including the epitopes formed by the gI/gE complex.

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Mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the gE gene from HSV-1

Cited by 17 publications

References 33 publications

Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit

Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit

Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

Epitopes on glycoprotein E and on the glycoprotein E/glycoprotein I complex of bovine herpesvirus 1 are expressed by all of 222 isolates and 11 vaccine strains

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