Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy

et al. 2017

Sensors

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The advent of atomic force microscopy (AFM) has provided a powerful tool for investigating the behaviors of single native biological molecules under physiological conditions. AFM can not only image the conformational changes of single biological molecules at work with sub-nanometer resolution, but also sense the specific interactions of individual molecular pair with piconewton force sensitivity. In the past decade, the performance of AFM has been greatly improved, which makes it widely used in biology to address diverse biomedical issues. Characterizing the behaviors of single molecules by AFM provides considerable novel insights into the underlying mechanisms guiding life activities, contributing much to cell and molecular biology. In this article, we review the recent developments of AFM studies in single-molecule assay. The related techniques involved in AFM single-molecule assay were firstly presented, and then the progress in several aspects (including molecular imaging, molecular mechanics, molecular recognition, and molecular activities on cell surface) was summarized. The challenges and future directions were also discussed.

“…By obtaining arrays of force curves on the cell surface, the distributions of receptors can be mapped [ 56 , 57 ]. However, this method is time-wasting with a low efficiency.…”

Section: Afm Single-molecule Techniquesmentioning

confidence: 99%

Imaging and Force Recognition of Single Molecular Behaviors Using Atomic Force Microscopy

Dang

et al. 2017

Sensors

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“…For the pole figure measurement to be realized, the hardware and software for motion control and data acquisition were specially designed by Xiaolong Liu et al [15]. Shown in Fig.…”

Section: Instrument Design and Characteristicsmentioning

confidence: 99%

“…Shown in Fig. 6 are the (0001), and (11)(12)(13)(14)(15)(16)(17)(18)(19)(20) pole figures, recalculated by J.R. Santisteban from the obtained ODF together with those measured at HIPPO and Kowari. Good agreement is found both qualitatively and quantitatively between the different instruments [16].…”

Section: Pole Figure Measurements For Warm-rolledmentioning

confidence: 99%

The neutron texture diffractometer at the China Advanced Research Reactor

et al. 2016

Chinese Phys. C

The first neutron texture diffractometer in China has been built at the China Advanced Research Reactor, due to strong demand for texture measurement with neutrons from the domestic user community. This neutron texture diffractometer has high neutron intensity, moderate resolution and is mainly applied to study texture in commonly used industrial materials and engineering components. In this paper, the design and characteristics of this instrument are described. The results for calibration with neutrons and quantitative texture analysis of Zirconium alloy plate are presented. The comparison of texture measurements with the results obtained in HIPPO at LANSCE and Kowari at ANSTO illustrates the reliability of the texture diffractometer.

“…Because of increasingly wider applications of AFM in life sciences, we now have a deeper understanding of physiological activities at the single-cell and single-molecule levels [36][37][38][39][40]. We can now obtain highresolution images of single membrane proteins and observe conformational changes.…”

Section: Challenge and Outlookmentioning

confidence: 99%

Progress of AFM single-cell and single-molecule morphology imaging

et al. 2013

Chin. Sci. Bull.

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Atomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallography. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed.