Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat

Torada, Atsushi; Ikeguchi, Shojiro; Koike, Michiya

doi:10.1007/s10681-005-7872-2

Cited by 80 publications

(50 citation statements)

References 13 publications

(9 reference statements)

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“…Noda et al (2002) investigated another ABA responsive gene located on the long arm of chromosome 4A. QPhs.spa-4A, located in interval barc170-gwm397-wmc617a on 4A, is in a region associated with dormancy and preharvest sprouting resistance in numerous other QTL studies of hexaploid wheat (Flintham et al 2002;Torada et al 2005;Mares et al 2005;Tan et al 2006;Ogbonnaya et al 2008;Imtiaz et al 2008;Munkvold et al 2009;Rasul et al 2009;Kulwal et al 2010;Singh et al 2010Singh et al , 2012Lohwasser et al 2013). The locus was also identified in tetraploid ) and diploid (Nakamura et al 2007) wheat populations, and in an association mapping study (Kulwal et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

et al. 2015

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Pre-harvest sprouting in spring wheat causes significant financial loss to growers throughout the world and sprouting damage can be reduced by growing resistant genotypes. Several genetic factors, especially those related to seed dormancy, are involved in the control of pre-harvest sprouting resistance. The objective of this study was to identify quantitative trait loci (QTL) influencing pre-harvest sprouting resistance from multiple measures of dormancy at multiple germination intervals on seed harvested across multiple environments. A doubled haploid mapping population of 91 individuals derived from a cross of two Canadian white-seeded spring wheat genotypes, SC8021-V2 (pre-harvest sprouting resistant) and AC Karma (moderately susceptible to pre-harvest sprouting) was used for QTL mapping. Daily germination counts were analysed using germination index, germination resistance and percent germination at intervals of 3, 5, 7, 10, 14 and 21 days from spike samples collected from six field and one greenhouse environments in Saskatchewan, Canada. Continuous frequency distributions at certain measure-durations indicated genetic complexity of dormancy segregation in the SC8021-V2/AC Karma cross. Composite interval mapping detected significant (p B 0.05) QTL associated with resistance to pre-harvest sprouting on all 21 wheat chromosomes. Of the 26 total QTL, six were novel and the rest were detected either at the same marker or overlapping a marker interval reported in other studies. QTL expressed consistently for germination index, germination resistance and percent germination at different germination durations on chromosomes 2B, 4A, 5D and 6D. QTL identified on homoeologous chromosomes 4A, 4B and 4D with chromosome specific molecular variants of SSR marker wmc617 suggest a conserved region for controlling dormancy on group four. The majority of QTL mapped in regions known to contain factors affecting different components of pre-harvest sprouting resistance like seed dormancy, seed coat colour, ABA responsiveness and alpha-amylase activity. This study demonstrated that using multiple measures of seed dormancy Electronic supplementary material The online version of this article (doi:10.1007/s10681-015-1460-x) contains supplementary material, which is available to authorized users. Euphytica (2015) 205:287-309 DOI 10.1007/s10681-015-1460 at multiple intervals of germination enhanced identification of QTL affecting dormancy in white-seeded hexaploid wheat.

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Section: Discussionmentioning

confidence: 99%

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

et al. 2015

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“…In earlier studies, grain dormancy has been shown to contribute to enhanced resistance to pre-harvest sprouting (Mares et al, 2009;Osa et al, 2003;Nakamura et al, 2007;Lohwasser et al, 2005;Torada et al, 2005;Ogbonnaya et al, 2008;Singh et al, 2010;Chen et al, 2008;Mares et al, 2005;Kottearachchi et al, 2008;Imtiaz et al, 2008;Munkvold et al, 2009;Fofana et al, 2009). This observation is confirmed by the results of the present study showing clustering of individual original QTLs for PHST and grain dormancy into a single cluster during meta-QTL analysis.…”

Section: Relationship Between Phst and Dormancymentioning

confidence: 99%

Meta-analysis of QTLs Involved in Pre-harvest Sprouting Tolerance and Dormancy in Bread Wheat

Tyagi¹,

Gupta²

2012

tgg

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In common wheat, meta-analysis of quantitative trait loci (QTL) associated with pre-harvest sprouting tolerance (PHST) and dormancy was carried out using results of 15 studies involving 15 different mapping populations. The study was restricted to only four chromosomes including three chromosomes of homoeologous group 3 (3A, 3B and 3D) and the chromosome 4A, since QTLs for PHST and dormancy on these four chromosomes were reported in several earlier studies, thus making these chromosomes suitable for meta-QTL analysis. The software BioMercator 2.1 was used to build a consensus map, and QTLs were projected on to this map for conducting meta-analysis. Using 36 of the 50 original QTLs, 8 meta-QTLs (MQTLs) were identified: 7 MQTLs were located on chromosomes of homoeologous group 3 including 3A (2 MQTL), 3B (3 MQTL) and 3D (2 MQTL), 1 MQTL was located on chromosome 4A. Confidence intervals (C.I.) for each of these 8 MQTLs were particularly narrow. The mapping information for 50 QTLs was also used for "overview" analysis to visualize important genomic regions carrying the MQTLs for PHST and dormancy. Co-localizations between candidate genes for dormancy/PHST (taVP1 and TaGA20-ox1) and MQTL positions appeared globally significant, although these candidate genes deserve further investigation. Markers associated with 8 MQTLs identified during the present study should prove helpful for introgression of tolerance against pre-harvest sprouting (PHS) into high yielding wheat varieties through marker-assisted selection (MAS).

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“…Kato et al (2001) detected a major QTL for PHS resistance on 4AL of a red wheat cultivar, and Mares and Mrva (2001) reported the same QTL, but with smaller eVect, for seed dormancy in white wheat. Several recent studies demonstrated that the major QTL on 4AL is present in both white and red wheat cultivars of diverse origins (Chen et al 2007;Lohwasser et al 2005;Mares et al 2005;Torada et al 2005). Although QTLs for PHS resistance have been identiWed on many chromosomes in diVerent studies, chromosome 4A and all long arms of group 3 chromosomes appear to be more critical for PHS resistance (Flintham and Gale 1996;Groos et al 2002;Kato et al 2001;Kulwal et al 2004;Mori et al 2005;Osa et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

et al. 2008

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Pre-harvest sprouting (PHS) of wheat is a major problem that severely limits the end-use quality of Xour in many wheat-growing areas worldwide. To identify quantitative trait loci (QTLs) for PHS resistance, a population of 171 recombinant inbred lines (RILs) was developed from the cross between PHS-resistant white wheat cultivar Rio Blanco and PHS-susceptible white wheat breeding line NW97S186. The population was evaluated for PHS in three greenhouse experiments and one Weld experiment. After 1,430 pairs of simple sequence repeat (SSR) primers were screened between the two parents and two bulks, 112 polymorphic markers between two bulks were used to screen the RILs. One major QTL, QPhs.pseru-3AS, was identiWed in the distal region of chromosome 3AS and explained up to 41.0% of the total phenotypic variation in three greenhouse experiments. One minor QTL, QPhs.pseru-2B.1, was detected in the 2005 and 2006 experiments and for the means over the greenhouse experiments, and explained 5.0-6.4% of phenotypic variation. Another minor QTL, QPhs.pseru-2B.2, was detected in only one greenhouse experiment and explained 4.5% of phenotypic variation for PHS resistance. In another RIL population developed from the cross of Rio Blanco/NW97S078, QPhs.pseru-3AS was signiWcant for all three greenhouse experiments and the means over all greenhouse experiments and explained up to 58.0% of phenotypic variation. Because Rio Blanco is a popular parent used in many hard winter wheat breeding programs, SSR markers linked to the QTLs have potential for use in high-throughput marker-assisted selection of wheat cultivars with improved PHS resistance as well as Wne mapping and map-based cloning of the major QTL QPhs.pseru-3AS.

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Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat

Cited by 80 publications

References 13 publications

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

Meta-analysis of QTLs Involved in Pre-harvest Sprouting Tolerance and Dormancy in Bread Wheat

Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

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