Mapping and functional analysis of candidate genes involved in resistance to soybean (Glycine max) mosaic virus strain SC3

Abstract

Soybean mosaic virus (SMV) infections can cause significant reduction in soybean yield and seed quality. Identifying SMV resistance genes in soybean and accordingly breeding resistant soybean varieties is an important approach to control soybean loss caused by SMV. Here, whole genome resequencing was performed on a pair of near‐isogenic line (NIL), one resistant (R) line and one susceptible (S) line to SC3, from Qihuang‐1 (resistant to SC3, RSC3Q) × Nannong 1138‐2 (susceptible to SC3, rSC3Q). Based on the distribution of single nucleotide polymorphisms (SNPs) and InDels in each chromosome, the RSC3Q was mapped to a region between 27.4 and 29.8 Mbp in chromosome 13. Comparing the sequence and transcriptome data between R line and susceptible lines (S line and Willams82) to SC3, three RSC3Q candidate genes (Glyma13g25950, Glyma13g25970 and Glyma13g26380) were identified. Silencing Glyma13g26380 in Qihuang‐1 increased the accumulation of SMV at 6 and 24 hpi. Subsequently, the SMV concentration in silenced plants decreased to the same level as that of the control. Thus, Glyma13g26380 is involved in the early resistance response of Qihuang No. 1 to SC3. These results provide a basis for elucidating the soybean resistance mechanism against SMV and breeding more resistant soybean varieties against SMV.

Discovery and Characterization of Differentially Expressed Soybean MiRNAs and Their Targets During Soybean Mosaic Virus Infection Unveils Novel Insight Into Soybean-SMV Interaction

“…Wu et al [58] mapped the resistance loci to bean common mosaic virus (BCMV) resistance genes mapped in the 58.1 kb interval between BARSOYSSR _13 _ 1114 and SNP-49 through positional cloning, including a CC-NBS-LRR type of resistance gene Glyma.13G184800, while Rsv1-h responsible to SMV was mapped to almost the same region as the previous SMV resistance allele, indicating that this gene may respond to both viruses. Previously, mapping and transcriptome sequencing analyses identi ed SC3 resistance gene candidates in chromosome 13 [52,54,59,60]. Zheng et al [54] found the SC3 resistance gene candidates involved in the process of disease resistance.…”

“…In particular, soybean contains many NBS-LRR genes, but limited studies were only conducted to describe the role of the NBS-LRR gene's involvement in resistance against pathogens. Several SMV resistance genes, including Rsv 1, R SC3Q , R SC11 , R SC14Q, and R SC20 were located at 4.295 Mb genomic region between 27 656 895-31 951 960 bp on chromosome 13 [51][52][53][54][55][56]. This genomic region is rich in NBS-LRR genes [57].…”

Extreme Resistance to Viruses in Potato and Soybean

“…The Rsv1 allele confers resistance to SMV strains G1-G6, but systemic necrosis occurs in response to SMV-G7 infection. These differences may be attributed to allelic differences in elicitor binding/recognition efficiencies but may also be due to non-NLR host factors (Yuan et al, 2020). The cause(s) of systemic necrosis is not well understood but may be due to the delayed activation of the immune response/HR (Hajimorad and Hill, 2001;Hajimorad et al, 2003;Seo et al, 2009).…”

Transcriptome-based discovery of genes and networks related to RSC3Q-mediated resistance to Soybean mosaic virus in soybean

“…The RNA-Seq data from the NILs (R line and S line) were obtained previously (Li et al 2017;Yuan et al 2020). In brief, leaves were collected as samples from the two lines independently at 0, 6, 20 and 48 h post-inoculation (hpi) with SC3.…”