Mapping and comprehensive analysis of the extracellular and cell surface proteome of the human pathogenCorynebacterium diphtheriae

Hansmeier, Nicole; Chao, Tzu-Chiao; Kalinowski, Jörn; Pühler, Alfred; Tauch, Andreas

doi:10.1002/pmic.200500360

Cited by 47 publications

(52 citation statements)

References 80 publications

(52 reference statements)

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“…Previous studies have revealed that various carbohydrate metabolism enzymes are commonly found on the surfaces of different bacterial species (47,(92)(93)(94)(95)(96)(97). Here we found that bile exposure caused several changes in the amounts of proteins related to carbohydrate metabolism in the surface-associated proteome of GG.…”

Section: Bile Affects Central Metabolic Processesmentioning

confidence: 50%

Proteomics and Transcriptomics Characterization of Bile Stress Response in Probiotic Lactobacillus rhamnosus GG

Koskenniemi

Laakso

Koponen

et al. 2011

Molecular & Cellular Proteomics

160

156

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Lactobacillus rhamnosus GG (GG) is a widely used and intensively studied probiotic bacterium. Although the health benefits of strain GG are well documented, the systematic exploration of mechanisms by which this strain exerts probiotic effects in the host has only recently been initiated. The ability to survive the harsh conditions of the gastrointestinal tract, including gastric juice containing bile salts, is one of the vital characteristics that enables a probiotic bacterium to transiently colonize the host. Here we used gene expression profiling at the transcriptome and proteome levels to investigate the cellular response of strain GG toward bile under defined bioreactor conditions. The analyses revealed that in response to growth of strain GG in the presence of 0.2% ox gall the transcript levels of 316 genes changed significantly (p < 0.01, t test), and 42 proteins, including both intracellular and surface-exposed proteins (i.e. surfome), were differentially abundant (p < 0.01, t test in total proteome analysis; p < 0.05, t test in surfome analysis). Protein abundance changes correlated with transcriptome level changes for 14 of these proteins. The identified proteins suggest diverse and specific changes in general stress responses as well as in cell envelope-related functions, including in pathways affecting fatty acid composition, cell surface charge, and thickness of the exopolysaccharide layer. These changes are likely to strengthen the cell envelope against bile-induced stress and signal the GG cells of gut entrance. Notably, the surfome analyses demonstrated significant reduction in the abundance of a protein catalyzing the synthesis of exopolysaccharides, whereas a protein dedicated for active removal of bile compounds from the cells was up-regulated. These findings suggest a role for these proteins in facilitating the well founded interaction of strain GG with the host mucus in the presence of sublethal doses of bile. The significance of these findings in terms of the functionality of a probiotic bacterium is discussed. Molecular & Cellular

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Section: Bile Affects Central Metabolic Processesmentioning

confidence: 50%

Proteomics and Transcriptomics Characterization of Bile Stress Response in Probiotic Lactobacillus rhamnosus GG

Koskenniemi

Laakso

Koponen

et al. 2011

Molecular & Cellular Proteomics

160

156

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show abstract

“…In the post genomic era, the availability of more and more improved methods for analyzing bacterial sub-proteomes has shed light on complex physiological phenomena such as the protein secretion mechanisms, 1 energy metabolism, 2 quorum sensing, 3 moonlighting proteins, 4,5 parallel catabolic pathways, 6 environmental adaptation 7 and detoxification mechanisms related to stress. 8,9 The analysis of extracellular and envelope-enriched proteomes can also supply information about exported virulence factors 10,11 useful for developing suitable diagnostics and therapeutics 12 and for discovering new interesting targets for vaccine development and antimicrobial chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Selenium effects on the metabolism of a Se-metabolizingLactobacillus reuteri: analysis of envelope-enriched and extracellular proteomes

et al. 2014

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a Selenium (Se) has received great attention in the last few years, as it is considered to be essential for human health (prevention of viral infections, heart diseases and ageing-related diseases). Se deficiency can be counteracted by the administration of selenium-enriched probiotics that are able to convert inorganic selenium into less toxic and more bio-available organic forms. This study was performed on Lactobacillus reuteri Lb2 BM DSM 16143, a probiotic LAB previously demonstrated to be able to fix Se into selenocysteines. The aim was to assess Se influence on its metabolism, by a 2-DE proteomic approach, on two different cellular districts: envelope-enriched and extracellular proteomes. While in the envelope-enriched fraction 15 differentially expressed proteins were identified, in the extracellular proteome no quantitative difference was detected. However, at a molecular level, we observed the insertion of Se into selenocysteine, exclusively under the stimulated conditions. The obtained results confirmed the possibility to use L. reuteri Lb2 BM DSM 16143 as a carrier of organic Se that can be easily released in the gut becoming available for the human host.

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“…Other actinobacteria have been reported to secrete substantially more proteins. Roughly 50% of the extracellular proteins of Corynebacterium diphtheriae had signal peptides, and 75% of supernatant proteins had signal peptides in Streptomyces lividans (10,14). The low degree of protein secretion in Frankia could be related to the medium used.…”

Section: Resultsmentioning

confidence: 99%

Diminished Exoproteome of Frankia spp. in Culture and Symbiosis

Mastronunzio

Huang

Benson

2009

Appl Environ Microbiol

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Frankia species are the most geographically widespread gram-positive plant symbionts, carrying out N 2 fixation in root nodules of trees and woody shrubs called actinorhizal plants. Taking advantage of the sequencing of three Frankia genomes, proteomics techniques were used to investigate the population of extracellular proteins (the exoproteome) from Frankia, some of which potentially mediate host-microbe interactions. Initial two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants indicated that cytoplasmic proteins appeared in supernatants as cells aged, likely because older hyphae lyse in this slow-growing filamentous actinomycete. Using liquid chromatography coupled to tandem mass spectrometry to identify peptides, 38 proteins were identified in the culture supernatant of Frankia sp. strain CcI3, but only three had predicted export signal peptides. In symbiotic cells, 42 signal peptidecontaining proteins were detected from strain CcI3 in Casuarina cunninghamiana and Casuarina glauca root nodules, while 73 and 53 putative secreted proteins containing signal peptides were identified from Frankia strains in field-collected root nodules of Alnus incana and Elaeagnus angustifolia, respectively. Solute-binding proteins were the most commonly identified secreted proteins in symbiosis, particularly those predicted to bind branched-chain amino acids and peptides. These direct proteomics results complement a previous bioinformatics study that predicted few secreted hydrolytic enzymes in the Frankia proteome and provide direct evidence that the symbiosis succeeds partly, if not largely, because of a benign relationship.

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Mapping and comprehensive analysis of the extracellular and cell surface proteome of the human pathogenCorynebacterium diphtheriae

Cited by 47 publications

References 80 publications

Proteomics and Transcriptomics Characterization of Bile Stress Response in Probiotic Lactobacillus rhamnosus GG

Proteomics and Transcriptomics Characterization of Bile Stress Response in Probiotic Lactobacillus rhamnosus GG

Selenium effects on the metabolism of a Se-metabolizingLactobacillus reuteri: analysis of envelope-enriched and extracellular proteomes

Diminished Exoproteome of Frankia spp. in Culture and Symbiosis

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