Mapping a new source of resistance to powdery mildew in mungbean

Abstract

Both restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) analyses were employed to map a new source of resistance to powdery mildew in mungbean. Disease scores of an F2 population derived from the cross between a moderately resistant breeding line VC1210A and a susceptible wild relative (Vigna radiata var. sublobata, accession TC1966) showed a continuous distribution and was treated as a quantitative trait. Although no significant quantitative trait loci (QTL) that can explain the variation was detected by QTL analysis based on the reconstructed RFLP linkage map, new marker loci associated with resistance were discovered by AFLP analysis. The RFLP loci detected by two of the cloned AFLP bands are associated with resistance and constitute a new linkage group. A major resistance quantitative trait locus was found on this linkage group that accounted for 64.9% of the variation in resistance to powdery mildew. One of the probes developed in this study has the potential to assist in breeding for powdery mildew resistance in mungbean.

Perspectives and Challenges for Sustainable Management of Fungal Diseases of Mungbean [Vigna radiata (L.) R. Wilczek var. radiata]: A Review

“…Several earlier studies reported monogenic inheritance of powdery mildew resistance controlled by single dominant genes and studies were conducted using mungbean varieties ML3 and ML5 (AVRDC, 1979), and breeding lines VC 1560A (AVRDC, 1981), ATF 3640 (Humphry et al, 2003) and RUM (Reddy et al, 1994a). Using restriction fragment length polymorphism (RFLP) markers, Chaitieng et al (2002) (Young et al, 1993;Sorajjapinun et al, 2005). Sorajjapinun et al (2005) reported that additive gene action was found to play a major role in controlling powdery mildew (E. polygoni) resistance in the population of crosses developed between moderately resistant KPS 2 and resistant VC 6468-11-1A (sourced from the World Vegetable Center).…”

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

“…Several molecular markers have been used in mungbean, including amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and inter-simple sequence repeats (ISSR) (Chaitieng et al, 2002;Afzal et al, 2004;Tangphatsornruang et al, 2009;Tantasawat et al, 2010b;Raturi et al, 2012). Afzal et al (2004) have used RAPD to evaluate the genetic diversity of 21 cultivated mungbeans from Thailand, Bangladesh, and India.…”

Screening techniques and sources of resistance to rusts and mildews in grain legumes

“…Both additive and dominant gene actions were found to be important as well as non-allelic interactions (Gawande & Patil, 2003). In lines VC1210 and ATF3640 major loci controlling resistance to this pathogen were detected using QTL analysis (Chai Tieng et al, 2002;Humphry et al, 2003). Molecular markers associated with resistance to powdery mildew of common bean have been identified (Melo et al, 2002).…”