Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple

Chagné, David; Carlisle, C. M.; Blond, Celine L. H.; Volz, Richard K.; Whitworth, Claire; Oraguzie, Nnadozie; Crowhurst, Ross N.; Allan, Andrew C.; Espley, Richard V.; Hellens, Roger P.; Gardiner, Susan E.

doi:10.1186/1471-2164-8-212

Cited by 193 publications

(124 citation statements)

References 49 publications

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“…This marker co-segregates with the Rni locus, which is positioned on LG 09, a region that has been previously implicated in the control of red skin color. Previous research has suggested that Rf and Rni might be located in a gene cluster, or even correspond to alleles of the same gene, because they are both located at the bottom of LG 09 on the apple genome (Maliepaard et al, 1998;Chagné et al, 2007). Moreover, in our study, the evaluation of 30 cultivars and two progenies produced consistent results for the 2 DNA markers ( Table 2, Table 4).…”

Section: Discussionsupporting

confidence: 83%

“…In apple, 2 other genes (MdMYB10 and MdMYBA), which are homologous to MdMYB1, have been isolated, and shown to regulate anthocyanin biosynthesis in apple skin or flesh (Takos et al, 2006;Ban et al, 2007;Espley et al, 2007). Chagné et al (2007) derived a molecular marker (NZms_MdMYB10) from MdMYB10, which is able to control red color in apple. This marker co-segregates with the Rni locus, which is positioned on LG 09, a region that has been previously implicated in the control of red skin color.…”

Section: Discussionmentioning

confidence: 99%

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A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection

Zhang¹,

Wang²,

Chen³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Pre-selection for fruit skin color at the seedling stage would be highly advantageous, with marker-assisted selection offering a potential method for apple pre-selection. A and MdMYB1 alleles are allele-specific DNA markers that are potentially associated with apple skin color, and co-segregate with the Rf and Rni loci, respectively. Here, we assessed the potential application of these 2 alleles for markerassisted breeding across 30 diverse cultivars and 2 apple seedling progenies. The red skin color phenotype was usually associated with the MdMYB1-1 allele and A 1 allele, respectively, while the 2 molecular markers provided approximately 91% predictability in the 'Fuji' x 'Cripps Pink' and 'Fuji' x 'Gala' progenies. The results obtained from the 30 cultivars and 2 progenies were consistent for the 2 molecular markers. Hence, the results supported that Rf and Rni could be located in a gene cluster, or even correspond to alleles of the same gene. Our results are consistent with the hypothesis that red/yellow dimorphism is controlled by a monogenic system, with the presence of the red anthocyanin pigmentation being dominant. In addition, our results supported that the practical utilization of the 2 function markers to efficiently and accurately select red-skinned apple cultivars in apple scion breeding programs.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection

Zhang¹,

Wang²,

Chen³

et al. 2014

Genet. Mol. Res.

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“…Only the QTL cluster on LG 9 (both parents) and on the cv Telamon LG 16 were specific for skin AsA concentrations. These regions on LG 9 and LG 16 have both been shown to contain major QTL for apple fruit polyphenolic contents (Chagné et al, 2007(Chagné et al, , 2012Khan et al, 2012). We have previously reported that the sunexposed (red) side of apples has higher anthocyanin and AsA concentrations and is more resistant to both biotic and abiotic stress than the shaded (green/ yellow) side (Davey et al, , 2007, and recently, Bulley et al (2012) demonstrated that transgenic fruits from tomato and strawberry with elevated AsA contents also had an approximately 50% larger polyphenolic pool.…”

Section: Discussion Antioxidant Concentrations and Qtl In The CV Txbmentioning

confidence: 98%

Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

et al. 2012

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To identify the genetic factors underlying the regulation of fruit vitamin C (L-ascorbic acid [AsA]) concentrations, quantitative trait loci (QTL) studies were carried out in an F1 progeny derived from a cross between the apple (Malus 3 domestica) cultivars Telamon and Braeburn over three years. QTL were identified for AsA, glutathione, total antioxidant activity in both flesh and skin tissues, and various quality traits, including flesh browning. Four regions on chromosomes 10, 11, 16, and 17 contained stable fruit AsA-QTL clusters. Mapping of AsA metabolic genes identified colocations between orthologs of GDP-L-galactose phosphorylase (GGP), dehydroascorbate reductase (DHAR), and nucleobase-ascorbate transporter within these QTL clusters. Of particular interest are the three paralogs of MdGGP, which all colocated within AsA-QTL clusters. Allelic variants of MdGGP1 and MdGGP3 derived from the cultivar Braeburn parent were also consistently associated with higher fruit total AsA concentrations both within the mapping population (up to 10-fold) and across a range of commercial apple germplasm (up to 6-fold). Striking differences in the expression of the cv Braeburn MdGGP1 allele between fruit from high-and low-AsA genotypes clearly indicate a key role for MdGGP1 in the regulation of fruit AsA concentrations, and this MdGGP allele-specific single-nucleotide polymorphism marker represents an excellent candidate for directed breeding for enhanced fruit AsA concentrations. Interestingly, colocations were also found between MdDHAR3-3 and a stable QTL for browning in the cv Telamon parent, highlighting links between the redox status of the AsA pool and susceptibility to flesh browning.

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“…Red-fleshed apples are not widely produced because of poor fruit quality (e.g., linkage group (LG) 9 (Chagné et al, 2007;Volz et al, 2013), and the other, MdMYB110a, is located on LG 17 Umemura et al, 2013). In apples with MdMYB10, designated as type 1, not only the flesh, but also other organs including the skin, flower, leaf, and stem, are red in color.…”

Section: Introductionmentioning

confidence: 99%

Thinning and Bagging Treatments and the Growing Region Influence Anthocyanin Accumulation in Red-fleshed Apple Fruit

Honda¹,

Iwanami²,

Naramoto³

et al. 2017

Hortic J

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In common apple cultivars with white flesh and red skin, it is known that fruit overload lowers fruit quality, and that skin anthocyanin concentrations are reduced by bagging treatment and warmer climatic conditions. In this study, the effects of these factors on anthocyanin accumulation in the flesh of red-fleshed apples were investigated using 'Geneva' and 'Pink Pearl' apple cultivars. Excess fruiting resulted in decreased anthocyanin concentration in the flesh and the titration acidity of the fruit in both cultivars. Fruit bagging treatments using double-layer paper bags revealed that anthocyanin was synthesized to a certain extent in the flesh under dark conditions in both cultivars. The treatment significantly reduced anthocyanin accumulation in the flesh of bagged 'Geneva' apples compared with unbagged fruit, but no inhibitory effect was clear in 'Pink Pearl' apples. In both cultivars, the anthocyanin concentration in the flesh of unbagged apples grown in sunlight was higher than that in unbagged apples grown in shade, whereas there was no difference between positions for bagged fruit. In apples grown in either sunlight or shade, no significant difference was found between anthocyanin concentrations in the sun-exposed side and shaded side of fruits. These results indicate that sunlight irradiation partially promoted anthocyanin accumulation in the flesh of unbagged 'Geneva' and 'Pink Pearl' apples and accelerated its accumulation on both the sun-exposed and the shaded side. A comparison of the fruit quality of 'Pink Pearl' apples grown in different climatic regions showed that apples harvested at Suzaka, a warmer region, were lower in firmness, starch index, and titration acidity and higher in soluble solids concentration compared with those from Morioka, a cooler region. The anthocyanin concentration in the flesh of 'Pink Pearl' apples from the trees grown at Morioka was more than ten-fold higher than that in apples from the trees grown at Suzaka. Our results suggest that an appropriate fruit load, growth in sunlight, and growth under cooler climatic conditions, may redden the flesh of red-fleshed apples, as is the case for common apples.

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Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple

Cited by 193 publications

References 49 publications

A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection

A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection

Allelic Variation in Paralogs of GDP-l-Galactose Phosphorylase Is a Major Determinant of Vitamin C Concentrations in Apple Fruit

Thinning and Bagging Treatments and the Growing Region Influence Anthocyanin Accumulation in Red-fleshed Apple Fruit

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