Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)

Deng, Xin; Haiqing, Zhang; Wang, Yue; He, Feng; Liu, Jinling; Xiao, Xiao; Shu, Zhifeng; Wei, Li; Guohuai, Wang; Wang, Guoliang

doi:10.1371/journal.pone.0099564

Cited by 77 publications

(77 citation statements)

References 45 publications

(47 reference statements)

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“…The leaf-color mutation is a good marker for screening the genetic purity of hybrid crops [13]. It has been helpful understanding of chlorophyll anabolism and catabolism, chloroplast development, and photosynthesis [18].…”

Section: Discussionmentioning

confidence: 99%

“…Due to the ease of identification, large numbers of leaf-color mutants have been described and characterized in many plant species like Arabidopsis thaliana [3], barley [4], maize [5], rice [6,7,8], soybean [9], sunflower [10], and wheat [11,12]. For example, in rice, more than 200 leaf color mutants have been documented, and 154 responsible genes have been mapped to all 12 chromosomes, of which 53 have been cloned [13]. In soybean, 25 nuclear genes affecting chlorophyll-deficiency have been identified and mapped [9].…”

Section: Introductionmentioning

confidence: 99%

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Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Han

Zhang

Wang

et al. 2016

IJMS

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Leaf color mutants are common in higher plants that can be used as markers in crop breeding or as an important tool in understanding regulatory mechanisms in chlorophyll biosynthesis and chloroplast development. In virescent leaf mutants, young leaves are yellow in color, which gradually return to normal green when the seedlings grow large. In the present study, we conducted phenotypic characterization and genetic mapping of the cucumber virescent leaf mutant 9110Gt conferred by the v-1 locus. Total chlorophyll and carotenoid content in 9110Gt was reduced by 44% and 21%, respectively, as compared with its wild type parental line 9110G. Electron microscopic investigation revealed fewer chloroplasts per cell and thylakoids per chloroplast in 9110Gt than in 9110G. Fine genetic mapping allowed for the assignment of the v-1 locus to a 50.4 kb genomic DNA region in chromosome 6 with two flanking markers that were 0.14 and 0.16 cM away from v-1, respectively. Multiple lines of evidence supported CsaCNGCs as the only candidate gene for the v-1 locus, which encoded a cyclic-nucleotide-gated ion channel protein. A single nucleotide change in the promoter region of v-1 seemed to be associated with the virescent color change in 9110Gt. Real-time PCR revealed significantly lower expression of CsaCNGCs in the true leaves of 9110Gt than in 9110G. This was the first report that connected the CsaCNGCs gene to virescent leaf color change, which provided a useful tool to establish linkages among virescent leaf color change, chloroplast development, chlorophyll biosynthesis, and the functions of the CsaCNGCs gene.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Han

Zhang

Wang

et al. 2016

IJMS

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“…Leaf color markers are widely used in maintaining the genetic purity of seeds and as a marker of self-pollination to monitor male sterile lines (Deng et al 2014;Lv et al 2013). The only phenotypic difference between wild type and the wlml1 mutant is a slight difference in plant height and 1000-grain weight (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…More than 180 color mutants were hitherto isolated and characterized in rice, and at least 80 genes (Deng et al 2014;Sun et al 2017). These mutations distributed in 12 chromosomes were cloned, most of which are controlled by a pair of recessive nuclear genes (Qian et al 1996;Wang et al 2008;Xie et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis and fine-mapping of a new rice mutant, white and lesion mimic leaf1

Chen

et al. 2018

Plant Growth Regul

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Rice (Oryza sativa L.) leaf color mutants are excellent models for studying chlorophyll biosynthesis and chloroplast development. In this study, we isolated a stable genetic white and lesion mimic leaf1 (wlml1) mutant from an ethyl methanesulfonate (EMS)-mutagenized population of the indica cultivar TN1. Compared with wild-type TN1, the wlml1 mutant had lower contents of chlorophyll and carotenoids, altered chloroplast ultrastructure, and altered regulation of genes associated with chlorophyll metabolism and chloroplast development. In addition, lesions formed on the leaves of wlml1 plants grown at 20 °C and genes related to disease resistance and antioxidant functions were up-regulated; by contrast, the mutant phenotype was partially suppressed at 28 °C. These findings indicated that WLML1 might play a role in chlorophyll metabolism and chloroplast development, as well as in biotic and abiotic stress responses. Genetic analysis showed that WLML1 was controlled by a recessive nuclear gene, and map-based cloning delimited WLML1 to a 159.7-kb region on chromosome 4 that includes 30 putative open reading frames. Based on these findings, the wlml1 mutant will be a good genetic material for further studies on chlorophyll metabolism and stress responses in rice.

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“…These studies identified that most of the leaf color mutants are controlled by a single recessive nuclear gene. For example, many leaf mutants of soybean[33], wheat [36], and rice [37] were all controlled by a single recessive nuclear gene. In addition, there are a few leaf color mutants, in which the traits are controlled by the cytoplasmic gene.…”

Section: Discussionmentioning

confidence: 99%

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Gao

Sang

Chen

et al. 2018

Preprint

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11Leaf color mutation in sesame always affects the growth and development of plantlets, and their 12 yield. To clarify the mechanisms underlying leaf color regulation in sesame, we analyzed a 13 yellow-green leaf mutant. Genetic analysis of the mutant selfing revealed 3 phenotypes-YY, 14 light-yellow (lethal); Yy, yellow-green; and yy, normal green-controlled by an incompletely 15 dominant nuclear gene, Siyl-1. In YY and Yy, the number and morphological structure of the 16 chloroplast changed evidently, with disordered inner matter, and significantly decreased 17 chlorophyll content. To explore the regulation mechanism of leaf color mutation, the proteins 18 expressed among YY, Yy, and yy were analyzed. All 98 differentially expressed proteins (DEPs) 19 were classified into 5 functional groups, in which photosynthesis and energy metabolism 20 (82.7%) occupied a dominant position. Our findings provide the basis for further molecular 21 mechanism and biochemical effect analysis of yellow leaf mutants in plants. 22 24which also contain numerous beneficial minerals, antioxidants, and multi-vitamins [1]. 25Compared with other oilseed crops, sesame is still a low yield crop with low harvest index. 26The key research objectives in sesame are to increase the photosynthesis efficiency and the 27 yield per square area. 28Leaf color is an important trait related with the chlorophyll content, and always affects the 29 photosynthesis efficiency and the final productivity [2]. In plants, the chloroplast structure 30 always alters the composition and content of photosynthetic pigments. Several studies on the 31 leaf color mutants have uncovered a wide range of leaf color mutation types [3]. Most 32 mutagenesis of leaf color were related to the structure and function of the chloroplast [4], 33 chlorophyll biosynthesis and degradation mechanisms [5], photosynthesis [6], chloroplast 34 developmental characteristics [7], genetics [8], and molecular mechanisms [9]. Furthermore, 35some mutants were used for crop breeding to higher biomass, quality, and/or other specific 36 characteristics [10]. There is a rich diversity of leaf color mutation types in plants [11][12][13][14]. 37Leaf color mutants were divided into five types according to the color classification albino, 38 yellowing, light-green, stripe, spot, etc [15, 16]. Falbel [12] divided the chlorophyll mutant 39 into two categories: (1) mutants that lack chlorophyll b, such as arabidopsis mutants [17], and 40(2) mutants with reduced synthesis of total chlorophyll and chlorophyll b; currently most 41 mutants belong to the latter category. Leaf color characteristics controlled by both nuclear 42 inheritance and cytoplasmic inheritance, may be a quantitative or a quality trait [18]. Most of 43 the leaf color variations are caused by nuclear gene mutation inducing a type of chlorophyll 44 deficiency in higher plants. The leaf color variations are mostly monogenic mutation, while a 45 handful of them are polygenic mutation. Among these mutations, the recessive mutations are 4...

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Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)

Cited by 77 publications

References 45 publications

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Genetic analysis and fine-mapping of a new rice mutant, white and lesion mimic leaf1

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

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