Maple and hickory leaf litter fungal communities reflect pre-senescent leaf communities

Liber, Julian A.; Minier, Douglas H.; Stouffer-Hopkins, Anna; Wyk, Judson Van; Longley, Reid; Bonito, Gregory

doi:10.7717/peerj.12701

Cited by 6 publications

(4 citation statements)

References 89 publications

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“…It suggests that phyllosphere microorganisms were the dominant groups in early decomposition, but as the decomposition progressed, soil bacteria entered the litter layer through raindrop splashing, wind action, and the carrying of fungal mycelium [31]. In this study, Proteobacteria, Actinobacteriota, and Acidobacteriota were the dominant nitrogen-fixing microorganisms in July and October, which is consistent with the results of soil bacterial communities in long-term time series [24], and exhibit environmental filtration [32]. Actinobacteria is a nutrient-poor bacterial community, which prefers nutrient-poor environments and has a higher concentration in extreme habitats [33].…”

Section: Differences In Bacterial Community Structure and Diversity I...supporting

confidence: 86%

Fast Bacterial Succession Associated with the Decomposition of Larix gmelinii Litter in Wudalianchi Volcano

Xie,

Cheng,

Cao

et al. 2024

Microorganisms

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In order to understand the role of microorganisms in litter decomposition and the nutrient cycle in volcanic forest ecosystems, the dominant forest species Larix gmelinii in the volcanic lava plateau of the Wudalianchi volcano was considered as the research object. We analyzed the response of bacterial community structure and diversity to litter decomposition for 1 year, with an in situ decomposition experimental design using litter bags and Illumina MiSeq high-throughput sequencing. The results showed that after 365 days, the litter quality residual rate of Larix gmelinii was 77.57%, and the litter N, P, C:N, C:P, and N:P showed significant differences during the decomposition period (p < 0.05). The phyla Cyanobacteria and the genus unclassified_o_Chloroplast were the most dominant groups in early decomposition (January and April). The phyla Proteobacteria, Actinobacteriota, and Acidobacteriota and the genera Massilia, Pseudomonas, and Sphingomona were higher in July and October. The microbial communities showed extremely significant differences during the decomposition period (p < 0.05), with PCoa, RDA, and litter QRR, C:P, and N as the main factors driving litter bacteria succession. Microbial functional prediction analysis showed that Chloroplasts were the major functional group in January and April. Achemoheterotrophy and aerobic chemoheterotrophy showed a significant decrease as litter decomposition progressed.

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Section: Differences In Bacterial Community Structure and Diversity I...supporting

confidence: 86%

Fast Bacterial Succession Associated with the Decomposition of Larix gmelinii Litter in Wudalianchi Volcano

Xie,

Cheng,

Cao

et al. 2024

Microorganisms

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“…It has been historically challenging to translate measurements of microbial phylogenetic diversity into predictions of functional diversity (Rodriguez et al ., 2009; Djemiel et al ., 2022) because phenotypic differences are difficult to predict from genetic sequence variation alone, especially among closely related individuals (Arnold & Lutzoni, 2007; Grünig et al ., 2008; Johnson et al ., 2012; Hazard et al ., 2017; U’Ren et al ., 2019; Harrison & Griffin, 2020; Liber et al ., 2022). To address this major gap, we compared the multivariate metabolic phenotypes of sister lineages of fungal endophytes and found that lineages harbor significant and selectable phenotypic variation related to growth on plant-derived metabolites and tissues.…”

Section: Discussionmentioning

confidence: 99%

Multivariate divergence in wild microbes: no evidence for evolution along a genetic line of least resistance

Gluck‐Thaler

Shaikh

Wood

2022

Preprint

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Fungal endophytes are ubiquitous residents within plant tissues. While taxonomic characterization of endophyte communities is common, we know little about how even closely related individuals differ functionally because fungal taxonomy is often a poor predictor of variation in ecological traits, including those related to the metabolism of plant-derived substrates. Here, we tested whether two fungal endophyte lineages from the same family (Mollisiaceae) sampled from the same host species (Vaccinium angustifolium) differ phenotypically by comparing metabolic trait means and correlations related to growth across a panel of plant phenolics, carbohydrates, and tissue extracts. We found that lineages harbor significant, selectable metabolic variation. While one lineage grew faster across nearly all substrates, lineages differed in their mean response to specific substrates. For example, lineages responded differently to phenolics and carbohydrates, most dramatically for caffeic acid which abolished differences between lineages. However, most correlations between substrates within lineages were positive, suggesting that metabolic evolution is generally unconstrained by trade-offs among the examined traits. Our findings illustrate that characterizing multivariate phenotypes- measuring multiple metabolic traits and the correlations between them- provides key insight into the process of endophyte evolution and reveals that both fungal-and plant-specific determinants shape opportunities for ecological differentiation within plant microbiomes.

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“…DNA from each isolate was extracted by placing a small amount of fungal tissue in an alkaline-Tris solution and incubating at 95°C for 10 min to lyse the cells ( 41 ). The supernatant was used for PCR amplification with the primers ITS1f and LR3 ( 42 , 43 ).…”

Section: Methodsmentioning

confidence: 99%

Terpenes modulate bacterial and fungal growth and sorghum rhizobiome communities

Chou,

Andersen,

Mechan Llontop

et al. 2023

Microbiol Spectr

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Terpenes are among the oldest and largest class of plant-specialized bioproducts that are known to affect plant development, adaptation, and biological interactions. While their biosynthesis, evolution, and function in aboveground interactions with insects and individual microbial species are well studied, how different terpenes impact plant microbiomes belowground is much less understood. Here we designed an experiment to assess how belowground exogenous applications of monoterpenes (1,8-cineole and linalool) and a sesquiterpene (nerolidol) delivered through an artificial root system impacted its belowground bacterial and fungal microbiome. We found that the terpene applications had significant and variable impacts on bacterial and fungal communities, depending on terpene class and concentration; however, these impacts were localized to the artificial root system and the fungal rhizosphere. We complemented this experiment with pure culture bioassays on responsive bacteria and fungi isolated from the sorghum rhizobiome. Overall, higher concentrations (200 µM) of nerolidol were inhibitory to Ferrovibrium and tested Firmicutes. While fungal isolates of Penicillium and Periconia were also more inhibited by higher concentrations (200 µM) of nerolidol, Clonostachys was enhanced at this higher level and together with Humicola was inhibited by the lower concentration tested (100 µM). On the other hand, 1,8-cineole had an inhibitory effect on Orbilia at both tested concentrations but had a promotive effect at 100 µM on Penicillium and Periconia . Similarly, linalool at 100 µM had significant growth promotion in Mortierella , but an inhibitory effect for Orbilia . Together, these results highlight the variable direct effects of terpenes on single microbial isolates and demonstrate the complexity of microbe-terpene interactions in the rhizobiome. IMPORTANCE Terpenes represent one of the largest and oldest classes of plant-specialized metabolism, but their role in the belowground microbiome is poorly understood. Here, we used a “rhizobox” mesocosm experimental set-up to supply different concentrations and classes of terpenes into the soil compartment with growing sorghum for 1 month to assess how these terpenes affect sorghum bacterial and fungal rhizobiome communities. Changes in bacterial and fungal communities between treatments belowground were characterized, followed by bioassays screening on bacterial and fungal isolates from the sorghum rhizosphere against terpenes to validate direct microbial responses. We found that microbial growth stimulatory and inhibitory effects were localized, terpene specific, dose dependent, and transient in time. This work paves the way for engineering terpene metabolisms in plant microbiomes for improved sustainable agriculture and bioenergy crop production.

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Maple and hickory leaf litter fungal communities reflect pre-senescent leaf communities

Cited by 6 publications

References 89 publications

Fast Bacterial Succession Associated with the Decomposition of Larix gmelinii Litter in Wudalianchi Volcano

Fast Bacterial Succession Associated with the Decomposition of Larix gmelinii Litter in Wudalianchi Volcano

Multivariate divergence in wild microbes: no evidence for evolution along a genetic line of least resistance

Terpenes modulate bacterial and fungal growth and sorghum rhizobiome communities

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