MAPKKK15 gene from shrimp Litopenaeus vannamei is transcribed in larva development stages and contributes to WSSV pathogenesis

Wang, Sheng; Li, Haoyang; Zhu, Peng; Fu, Qihui; Yin, Bin; Li, Qinyao; Chen, Rongjian; Jiang, Xiewu; Weng, Shaoping; He, Jianguo; Li, Chaozheng

doi:10.1016/j.aquaculture.2020.736324

Cited by 4 publications

(2 citation statements)

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“…The open reading frames (ORFs) of L. vannamei Dorsal-L and Dorsal-S were amplified and cloned into pAc5.1-3×HA vector (24) to express the HA-tagged Dorsal-L protein and Dorsal-S proteins, respectively. Extracellular (ec) domain of each Toll (Toll1/2/3/4/5/ 6/7/8/9-ec), and the cytoplasmic TIR domain of each Toll (TIR1/2/3/ 4/5/6/7/8/9) were constructed into pAc5.1-3×HA vector to express HA-tagged proteins.…”

Section: Plasmids Constructionmentioning

confidence: 99%

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Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp

Wang

Li²,

Li³

et al. 2023

Front. Immunol.

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Toll-like receptors (TLR) play a crucial role in the detection of microbial infections in vertebrates and invertebrates. Mammalian TLRs directly recognize a variety of structurally conserved microbial components. However, invertebrates such as Drosophila indirectly recognize microbial products by binding to the cytokine-like ligand Spätzle, which activates signaling cascades that are not completely understood. In this study, we investigated the signaling events triggered by Toll in response to lipopolysaccharide (LPS), a cell wall component of gram-negative bacteria, and Vibrio parahaemolyticus infection in the arthropod shrimp Litopenaeus vannamei. We found that five of the nine Tolls from L. vannamei bound to LPS and the RNAi of LvToll1, LvToll2, LvToll3, LvToll5, and LvToll9 weakened LvDorsal-L phosphorylation induced by V. parahaemolyticus. All nine Tolls combined with MyD88 via the TIR domain, thereby conferring signals to the tumor necrosis factor receptor-associated factor 6 (TRAF6)-transforming growth factor-β activated kinase 1 binding protein 2 (TAB2)-transforming growth factor-β activated kinase 1 (TAK1) complex. Further examination revealed that the LvTRAF6-LvTAB2-LvTAK1 complex contributes to Dorsal-L phosphorylation and nuclear translocation during V. parahaemolyticus infection. Overall, shrimp Toll1/2/3/5/9–TRAF6/TAB2/TAK1–Dorsal cascades protect the host from V. parahaemolyticus infection, which provides a better understanding of how the innate immune system recognizes and responds to bacterial infections in invertebrates.

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Section: Plasmids Constructionmentioning

confidence: 99%

“…The Dual-luciferase reporter assays were done as our previous described (24) and measured with Dual-Glo Luciferase Assay System kit (Promega, cat. No.…”

Section: Dual Luciferase Reporter Assaymentioning

confidence: 99%