MAPK cascade signalling networks in plant defence

Pitzschke, Andrea; Schikora, Adam; Hirt, Heribert

doi:10.1016/j.pbi.2009.06.008

Cited by 629 publications

(533 citation statements)

References 55 publications

Supporting

Mentioning

507

Contrasting

Unclassified

Order By: Relevance

“…The MEKK1-MKK1/2-MPK4 pathway has been established as a negative regulator of SA and ROS signaling in Arabidopsis (Pitzschke et al, 2009a). Increased SA and ROS accumulation not only trigger cell death and defense, but they are also the consequence of the activation of MAPK signaling pathways (Yoshioka et al, 2003;Rentel and Knight, 2004;Nakagami et al, 2006;Pitzschke et al, 2009b). Here, we used BPMV-VIGS of GmMPK4s to test their function in soybean.…”

Section: Gmmpk4 Negatively Controls Sa and H 2 O 2 Levelsmentioning

confidence: 99%

“…Activation of mitogen-activated protein kinase (MAPK) cascades is a conserved mechanism for regulating innate immune responses in all eukaryotes (Pitzschke et al, 2009b). A MAPK signaling module consists of three protein kinases sequentially activated through phosphorylation by the upstream component: a MAP kinase kinase kinase (MAPKKK or MEKK), a MAP kinase kinase (MAPKK or MKK), and a MAP kinase (MPK;Mészáros et al, 2006).…”

mentioning

confidence: 99%

“…There are more than 80 putative MAPKKKs, 10 MAPKKs, and at least 20 MPKs in the Arabidopsis (Arabidopsis thaliana) genome (Ichimura et al, 2002). Among the 20 Arabidopsis MPKs, MPK3, MPK4, and MPK6 are implicated in defense responses and have been most extensively studied (Innes, 2001;Pitzschke et al, 2009b). While MPK3 and MPK6 act as positive regulators of defense responses (Asai et al, 2002;Menke et al, 2005;Takahashi et al, 2007;Ren et al, 2008;Mao et al, 2011), MPK4 negatively regulates defense responses (Petersen et al, 2000;Brodersen et al, 2006;Gao et al, 2008).…”

mentioning

confidence: 99%

“…bean genes involved in defense and other processes, such as MAPKs, that regulate plant disease resistance in model plants (Innes, 2001;Pedley and Martin, 2005;Pitzschke et al, 2009b). Based on BLAST searches, there are at least 56 MAPKs, 80 MAPKKs, and over 100 MAPKKKs in the soybean genome (www.phytozome.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development

et al. 2011

View full text Add to dashboard Cite

Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species such as Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum). However, the importance of MAPK signaling pathways in the disease resistance of crops is still largely uninvestigated. To better understand the role of MAPK signaling pathways in disease resistance in soybean (Glycine max), 13, nine, and 10 genes encoding distinct MAPKs, MAPKKs, and MAPKKKs, respectively, were silenced using virus-induced gene silencing mediated by Bean pod mottle virus. Among the plants silenced for various MAPKs, MAPKKs, and MAPKKKs, those in which GmMAPK4 homologs (GmMPK4s) were silenced displayed strong phenotypes including stunted stature and spontaneous cell death on the leaves and stems, the characteristic hallmarks of activated defense responses. Microarray analysis showed that genes involved in defense responses, such as those in salicylic acid (SA) signaling pathways, were significantly up-regulated in GmMPK4-silenced plants, whereas genes involved in growth and development, such as those in auxin signaling pathways and in cell cycle and proliferation, were significantly down-regulated. As expected, SA and hydrogen peroxide accumulation was significantly increased in GmMPK4-silenced plants. Accordingly, GmMPK4-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants. Using bimolecular fluorescence complementation analysis and in vitro kinase assays, we determined that GmMKK1 and GmMKK2 might function upstream of GmMPK4. Taken together, our results indicate that GmMPK4s negatively regulate SA accumulation and defense response but positively regulate plant growth and development, and their functions are conserved across plant species.

show abstract

Section: Gmmpk4 Negatively Controls Sa and H 2 O 2 Levelsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development

et al. 2011

View full text Add to dashboard Cite

show abstract

“…It is well established that plant MAPK cascades play key functions in defense responses (Pitzschke et al, 2009). MAPK signaling is mediated at the transcriptional, translational, and posttranslational levels (Beckers et al, 2009) and was for instance shown to promote the synthesis of camalexin, an antimicrobial compound (Ren et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Stress-Responsive Mitogen-Activated Protein Kinases Interact with the EAR Motif of a Poplar Zinc Finger Protein and Mediate Its Degradation through the 26S Proteasome

Hamel

Benchabane²,

Nicole³

et al. 2011

Plant Physiology

View full text Add to dashboard Cite

Mitogen-activated protein kinases (MAPKs) contribute to the establishment of plant disease resistance by regulating downstream signaling components, including transcription factors. In this study, we identified MAPK-interacting proteins, and among the newly discovered candidates was a Cys-2/His-2-type zinc finger protein named PtiZFP1. This putative transcription factor belongs to a family of transcriptional repressors that rely on an ERF-associated amphiphilic repression (EAR) motif for their repression activity. Amino acids located within this repression motif were also found to be essential for MAPK binding. Close examination of the primary protein sequence revealed a functional bipartite MAPK docking site that partially overlaps with the EAR motif. Transient expression assays in Arabidopsis (Arabidopsis thaliana) protoplasts suggest that MAPKs promote PtiZFP1 degradation through the 26S proteasome. Since features of the MAPK docking site are conserved among other EAR repressors, our study suggests a novel mode of defense mechanism regulation involving stress-responsive MAPKs and EAR repressors.

show abstract

Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants

et al. 2017

View full text Add to dashboard Cite

Plant growth flexibly adapts to environmental conditions, implying cross‐talk between environmental signalling and developmental regulation. Here, we show that the PIN auxin efflux carrier family possesses three highly conserved putative mitogen‐activated protein kinase (MAPK) sites adjacent to the phosphorylation sites of the well‐characterised AGC kinase PINOID, which regulates the polar localisation of PINs and directional auxin transport, thereby underpinning organ growth. The conserved sites of PIN1 are phosphorylated in vitro by two environmentally activated MAPKs, MPK4 and MPK6. In contrast to AGC kinases, MAPK‐mediated phosphorylation of PIN1 at adjacent sites leads to a partial loss of the plasma membrane localisation of PIN1. MAPK‐mediated modulation of PIN trafficking may participate in environmental adjustment of plant growth.

show abstract

MAPK cascade signalling networks in plant defence

Cited by 629 publications

References 55 publications

Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development

Soybean Homologs of MPK4 Negatively Regulate Defense Responses and Positively Regulate Growth and Development

Stress-Responsive Mitogen-Activated Protein Kinases Interact with the EAR Motif of a Poplar Zinc Finger Protein and Mediate Its Degradation through the 26S Proteasome

Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants

Contact Info

Product

Resources

About