MAP3K10 promotes the proliferation and decreases the sensitivity of pancreatic cancer cells to gemcitabine by upregulating Gli-1 and Gli-2

An, Yong; Cai, Baobao; Chen, Jianmin; Lv, Nan; Yao, Jie; Xue, Xiaofeng; Tu, Min; Tang, Dong; Wei, Jishu; Jiang, Kuirong; Wu, Junli; Li, Qiang; Gao, Wentao

doi:10.1016/j.canlet.2012.11.005

Cited by 21 publications

(16 citation statements)

References 46 publications

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“…MAP3K10 was also indicated to be a target gene of miR-155-5p. MAP3K10 is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, it activates the C-Jun N-terminal kinase (JNK) signaling pathway and the p38 mitogen-activated-protein kinase (MAPK) pathway, and regulates apoptosis in numerous neurodegenerative diseases (32)(33)(34). It was demonstrated in the present study that MAP3K10 may be targeted by miR-155-5p in GC cell lines.…”

Section: Discussionmentioning

confidence: 64%

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The tumor suppressor role of miR‑155‑5p in gastric cancer

Zhang

Zhou

et al. 2018

Oncol Lett

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Gastric cancer (GC) is the fifth most common type of malignant tumor worldwide and the most common cause of cancer-associated mortality in China. Recent studies revealed that microRNAs (miRNAs) function in the pathogenesis of GC, and that miR-155-5p expression is downregulated in GC tissues. However, the function of miR-155-5p has not been fully identified. In the present study, it was demonstrated that overexpression of miR-155-5p inhibited GC-cell proliferation and promoted apoptosis, while downregulation of miR-155-5p promoted GC-cell proliferation and decreased the cisplatin sensitivity of GC cells. Mitogen-activated protein kinase kinase kinase 10 was demonstrated to be a potential target gene of miR-155-5p. In conclusion, an antitumor role of miR-155-5p in gastric cancer was suggested.

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Section: Discussionmentioning

confidence: 64%

“…A previous study demonstrated that MAP3K10 was overexpressed in pancreatic ductal adenocarcinoma (PDAC), and that it promoted proliferation and decreased therapeutic impact in pancreatic cancer cells (32). Bioinformatics analysis suggested that MAP3K10 is a targeted gene of miR-155-5p (Fig.…”

Section: Map3k10 Is Targeted By Mir-155-5pmentioning

confidence: 94%

The tumor suppressor role of miR‑155‑5p in gastric cancer

Zhang

Zhou

et al. 2018

Oncol Lett

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“…GLI2 is located in 2q14, a member of the zinc finger transcription factor GLI family. Studies found that there was increased GLI2 activity in gastric cancer, lung squamous cell, pancreatic and basal cell carcinoma, and melanoma and other tumors, possibly by the activation of hedgehog signaling pathway to promote tumor invasion and induce chemoresistance (52)(53)(54)(55)(56). PLCG1, also called PLCγ1, is located in 20q12-q13.1.…”

Section: Discussionmentioning

confidence: 99%

Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction

et al. 2015

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Gastric signet ring cell carcinoma (GSRCC) is a unique pathological type of gastric carcinoma that is extremely invasive and has a poor prognosis after diagnosis. The expression of microRNAs has been closely linked to the carcinogenesis of gastric cancer and has been considered as a powerful prognostic marker. Distinctive expression of miRNAs in GSRCC was investigated in the present study. Samples of GSRCC were compared to that of intestinal gastric adenocarcinoma using Agilent microarray technique, and two differentially expressed miRNAs were identified, hsa-miR-665 and hsa-miR‑95. qRT-PCR verification showed downregulation of both miRNAs in signet ring cell carcinoma and upregulation in gastric adenocarcinoma, which was not consistent with the results obtained by the microarray. Target gene prediction using online databases conferred two strong candidate genes, GLI2 and PLCG1. GO/KO analysis of these two genes showed close correlations with carcinogenesis and chemoresistance. It was concluded that hsa-miR-665 and hsa-miR-95 were downregulated in GSRCC but upregulated in intestinal gastric adenocarcinoma, and the relatively differential expression of the miRNAs negatively controlling their target genes could be closely related to the high invasive metastasis and chemoresistance of GSRCC.

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“…Cells were cultured with gemcitabine (25nM or 50nM) for 48 h [15]. Cells were collected, washed with PBS, and suspended in 100 μL binding buffer, stained with 5μL of Phycoerythrin (PE)-Annexin-V and 5 μL of 7-AAD for 15 min in the dark.…”

Section: Analysis Of Cellular Apoptosismentioning

confidence: 99%

Vasohibin 2 reduces chemosensitivity to gemcitabine in pancreatic cancer cells via JUN proto-oncogene dependent transactivation of ribonucleotide reductase regulatory subunit M2

et al. 2017

Pancreatology

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MAP3K10 promotes the proliferation and decreases the sensitivity of pancreatic cancer cells to gemcitabine by upregulating Gli-1 and Gli-2

Cited by 21 publications

References 46 publications

The tumor suppressor role of miR‑155‑5p in gastric cancer

The tumor suppressor role of miR‑155‑5p in gastric cancer

Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction

Vasohibin 2 reduces chemosensitivity to gemcitabine in pancreatic cancer cells via JUN proto-oncogene dependent transactivation of ribonucleotide reductase regulatory subunit M2

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